We narrowed to 16,532 results for: puromycin

1. pLenti-PGK-RAP1A-E63-puro

   Plasmid

   #156167

   Purpose

   Lentiviral vector for expression of RAP1A-E63, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1A (RAP1A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   changed Glutamine 63 to Glutamic acid

   Promoter

   human PGK

   Available Since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-TetOne-Puro-MCL1

   Plasmid

   #202663

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP

   Plasmid

   #132393

   Purpose

   Exchange cassette containing inducible TauP301L 2N4R-EGFP fusion, CAG-driven rtTA3, Puromycin resistance marker, targets AAVS1 site

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   human TauP301L 2N4R-EGFP (MAPT Synthetic, Human)
   

   Use

   CRISPR and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Proline 301 to Leucine (P301L)

   Promoter

   TREtight

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPaint-mScarlet-PuroR [M1G]

   Plasmid

   #171809

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-FLAG-2xSTREP-CDKN1B-Puro

   Plasmid

   #172611

   Purpose

   Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDKN1B (CDKN1B Human)
   

   Use

   Retroviral

   Tags

   FLAG-2xSTREP

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRIPZ-CTID195-GSQ-UBnc-PURO

   Plasmid

   #208037

   Purpose

   Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   Ubnc (UBC Human)
   

   Use

   Lentiviral

   Tags

   Myc, C-terminal Split-TurboID

   Mutation

   L73P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

   Plasmid

   #208035

   Purpose

   Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

   Insert

   SUMO1nc (SUMO1 Human)
   

   Use

   Lentiviral

   Tags

   Myc, C-terminal Split-TurboID

   Mutation

   Q94P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPaint-mNeon-PuroR [M1G]

   Plasmid

   #171800

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-T2A-PuroR
   

   Use

   Gene tagging

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. retro-puro vector

   Plasmid

   #58250

   Purpose

   Retroviral expression vector encoding an empty IRES-Puromycin cassette

   Depositor

   Georgios Stathopoulos

   Article

   Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   March 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

    Plasmid

    #207355

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMV Puro DEST (w118-1) eGFP

    Plasmid

    #107505

    Purpose

    Expresses eGFP, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRIPZ-bioGEF-SUMO1nc-PURO

    Plasmid

    #208042

    Purpose

    Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

    Insert

    SUMO1nc (SUMO1 Human)
    

    Use

    Lentiviral

    Tags

    Low-affinity Avitag

    Mutation

    Q94P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-FLAG-2xSTREP-CCND3(T283A)-Puro

    Plasmid

    #172618

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3(T283A) and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Thr283Ala

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. retro-gfp-puro vector

    Plasmid

    #58249

    Purpose

    Retroviral expression vector encoding bicistronic EGFP and Puromycin cassette

    Depositor

    Georgios Stathopoulos

    Article

    Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

    Insert

    EGFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMSCV-FLAG-2xSTREP-CCND3-Puro

    Plasmid

    #172619

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMSCV-FLAG-2xSTREP-CCND2-Puro

    Plasmid

    #172626

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-PGK-flag-RAP1B-V12-puro

    Plasmid

    #156168

    Purpose

    Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1B (RAP1B Bovine)
    

    Use

    Lentiviral

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Valine

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.puro_shGLS

    Plasmid

    #110335

    Purpose

    shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

    Depositor

    Sandra Martha Gomes Dias

    Article

    Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

    Insert

    GLS glutaminase (GLS Synthetic, Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA PolIII)

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBABE-2xFLAG-2xSTREP-CCND2-Puro

    Plasmid

    #172628

    Purpose

    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBABE-2xFLAG-2xSTREP-CCND3-Puro

    Plasmid

    #172622

    Purpose

    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Retroviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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