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We narrowed to 17,698 results for: puromycin

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Showing: 1361 - 1380 of 17698 results

  1. pICE-HA-MRE11-H63D

    Plasmid
    #82036
    Purpose
    Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Sébastien Britton, Patrick Calsou
    Publication
    Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MRE11 (MRE11 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Exonuclease-dead mutant: H63D MRE11
    Promoter
    CMV-tet
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    82036-plasmid-map-sequence-id-155389

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  2. TRIPZ-bioGEF-UBnc-PURO

    Plasmid
    #208044
    Purpose
    Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Publication
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    UBnc (UBC Human)
    Use
    Lentiviral
    Tags
    Low-affinity Avitag
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    208044-plasmid-map-sequence-id-410266

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  5. pRDA_118

    Plasmid
    #133459
    Purpose
    U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR
    Depositor
    John Doench
    Publication
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    133459-plasmid-map-sequence-id-261403

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  8. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid
    #123363
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.
    Depositor
    Sidi Chen
    Publication
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    EGFP-NLS
    Use
    CRISPR, Lentiviral, and Luciferase
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    123363-plasmid-map-sequence-id-240469

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  13. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Publication
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    117686-plasmid-map-sequence-id-236350

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  14. pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro

    Plasmid
    #189635
    Purpose
    Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries
    Depositor
    Manuel Kaulich
    Publication
    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.
    Insert
    h7SK arrayed Cas12a gRNA cassette, hU6 arrayed Cas12a gRNA cassette, PGK puro cassette
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    189635-plasmid-map-sequence-id-414905

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  15. pKF-P14MMP2AG-T2APuroR-5h

    Plasmid
    #128256
    Purpose
    Mammalian positive feedback gene circuit in a Flp-In expression system controlling the PuroR gene. Also called mPF-PuroR.
    Depositor
    Gabor Balazsi
    Publication
    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rtTA-Advanced::P2A::EGFP::T2A::PuroR
    Use
    Synthetic Biology; Flp-in expression vector
    Expression
    Mammalian
    Promoter
    tight TRE promoter
    Available since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    128256-plasmid-map-sequence-id-248948

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  18. PLVX_SPTA1_IGF2BP1_IRES_PURO

    Plasmid
    #100016
    Purpose
    To over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.
    Depositor
    Jeffery Miller
    Publication
    de Vasconcellos et al Proc Natl Acad Sci U S A. 2017 Jul 11;11
    Insert
    Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SPTA1 erythroid specific promoter
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100016-plasmid-map-sequence-id-194507

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Showing: 1361 - 1380 of 17698 results