We narrowed to 325 results for: staphylococcus
-
Plasmid#199807Purposevector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureusDepositorInserttransposon cassette
ExpressionBacterialAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTH3
Plasmid#84453Purposeshuttle plasmid for sarA P1-YFP expressionDepositorInsertYFP venus
UseShuttle vector e.coli-s.aureus, expression of sgf…Available SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
LTR5HS S. aureus scaffold CARGO array
Plasmid#191317PurposeContains an array of guide RNAs targeting LTR5Hs elementsDepositorInsertArray of guide RNAs
UseCRISPRTagsmCherryExpressionMammalianAvailable SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRN12
Plasmid#84456Purposeshuttle plasmid for sarA P1-mAmetrine expressionDepositorInsertmAmetrine
UseShuttle vector e.coli-s.aureus, expression of sgf…Available SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCN34e-kimA-yfp
Plasmid#227638Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.DepositorInsertkimA-yfp
ExpressionBacterialAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRU55
Plasmid#167684PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRN10
Plasmid#84454Purposeshuttle plasmid for sarA P1-mKate expressionDepositorInsertmKate2
UseShuttle vector e.coli-s.aureus, expression of sgf…Available SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCN34e-ktrA-yfp
Plasmid#227640Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.DepositorInsertktrA-yfp
ExpressionBacterialAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTH1
Plasmid#84451Purposeshuttle plasmid for sarA P1-dsRED expressionDepositorInsertDsRed.T3(DNT)
UseShuttle vector e.coli-s.aureus, expression of sgf…Available SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCN38-ktrA-yfp
Plasmid#227636Purposec-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.DepositorInsertktrA-yfp
ExpressionBacterialAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEPSA5Cas9syn2
Plasmid#206963PurposeS. aureus genome editing vector removed these two motifs AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and GWAGNNNNNNGATDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SpSrtA
Plasmid#64987PurposeExpresses the wild type of Streptococcus pyogenes sortase A in E. coliDepositorInsertS. pyogenes sortase A
TagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-81PromoterT7 promoterAvailable SinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRU206
Plasmid#167693PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUBQ4-2::asCpf1
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU322
Plasmid#167683PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCas9
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPSA5Cas9syn
Plasmid#206965PurposeS. aureus genome editing vector removed these two motifs AGGNNNNNGAT and CCAYNNNNNNTGTDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRU61
Plasmid#167692PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::asCpf1
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mammalian_SaAID3S
Plasmid#188651PurposeExpresses SaAID3S and gRNA in mammalian cellsDepositorInsertsnSaCas9-AID3S--UGI
Sa-sgRNA
TagsUGIExpressionMammalianMutationD10A for SaCas9PromoterEF-1a and H1Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mammalian_SaAID2S
Plasmid#188652PurposeExpresses SaAID2S and gRNA in mammalian cellsDepositorInsertsAID2S-nSaCas9--UGI
Sa-sgRNA
TagsUGIExpressionMammalianMutationD10A for SaCas9PromoterEF-1a and H1Available SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCF824_U6-Sau-sgRNA-EF1a-mCherry2_lenti
Plasmid#138318PurposeLenti expression of mCherry2 with U6 promoter for SauCas9 sgRNA targeting GFPDepositorInsertSauCas9 GFP sgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRU205
Plasmid#167685PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUBQ4-2::asCpf1
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU321
Plasmid#167681PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUB4-2::asCas9
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU320
Plasmid#167682PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUBQ4-2::asCas9
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCF820_U6-sgRNA-EF1a-mCherry2_lenti
Plasmid#138316PurposeLenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFPDepositorInsertSpyCas9 GFP sgRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
P. falciparum DHPS
Plasmid#64080PurposeExpression of P. falciparum dihydropteroate synthaseDepositorInsertdihydropteroate synthase
TagsHistidine tagAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
sortase A pentamutant (eSrtA) in pET29
Plasmid#75144Purposeevolved sortase A (eSrtA) pentamutant with improved kinetics and activityDepositorInsertsortase A pentamutant
Tags6x HisExpressionBacterialMutationP94R, D160N, D165A, K190E, K196T. Deleted amino a…PromoterT7Available SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
SaCas9
Plasmid#188494PurposeExpresses FLAG tagged SaCas9DepositorInsertCas9
UseCRISPR and LentiviralExpressionMammalianPromoterEF1aAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWaldo-GFPe-SH1446
Plasmid#112507PurposeExpresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.DepositorInsertPOT family transporter
TagsC terminal TEV GFP octa-His affinity tagExpressionBacterialPromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
BA31_pU.CAG.SaCas9-D10A.rBGpA
Plasmid#199251PurposeExpression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)DepositorInsertSaCas9.D10A nickase
UseCRISPRTags3XHA epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_12
Plasmid#60583Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_12
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_8
Plasmid#60579Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_8
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only