Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 9,389 results for: Coli

Showing: 301 - 350 of 9389 results

  1. pGBW-m4046747

    Plasmid
    #149249
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149249-plasmid-map-sequence-id-358332

    Enlarge

  2. pGBW-m4046733

    Plasmid
    #145665
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145665-plasmid-map-sequence-id-292478

    Enlarge

  3. pGBW-m4046789

    Plasmid
    #145660
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145660-plasmid-map-sequence-id-298333

    Enlarge

  4. pGBW-m4046921

    Plasmid
    #145755
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145755-plasmid-map-sequence-id-292520

    Enlarge

  5. pGBW-m4046925

    Plasmid
    #145694
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145694-plasmid-map-sequence-id-313545

    Enlarge

  6. pGBW-m4046952

    Plasmid
    #145718
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145718-plasmid-map-sequence-id-307567

    Enlarge

  7. pGBW-m4046726

    Plasmid
    #145756
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145756-plasmid-map-sequence-id-298374

    Enlarge

  8. pGBW-m4046945

    Plasmid
    #145668
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145668-plasmid-map-sequence-id-300780

    Enlarge

  9. pGBW-m4046938

    Plasmid
    #145599
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145599-plasmid-map-sequence-id-300265

    Enlarge

  10. pGBW-m4046926

    Plasmid
    #145663
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145663-plasmid-map-sequence-id-352066

    Enlarge

  11. pGBW-m4046970

    Plasmid
    #145753
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145753-plasmid-map-sequence-id-311539

    Enlarge

  12. pGBW-m4046908

    Plasmid
    #145603
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145603-plasmid-map-sequence-id-298366

    Enlarge

  13. pGBW-m4046878

    Plasmid
    #145743
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145743-plasmid-map-sequence-id-298347

    Enlarge

  14. pGBW-m4046904

    Plasmid
    #145591
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145591-plasmid-map-sequence-id-298327

    Enlarge

  20. pBEL2265

    Plasmid
    #175962
    Purpose
    For expresson of LetA-[LetB PLL3->6] operon from E. coli. N-terminal his tag on LetA, araBAD promoter
    Depositor
    Damian Ekiert, Gira Bhabha
    Article
    Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d
    Inserts
    LetA
    LetB
    Use
    Tags
    8xHis 2xQH and TEV cleavage site on LetA
    Expression
    Bacterial
    Mutation
    Δ347-361, replace with 702-716
    Promoter
    araBAD
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    175962-plasmid-map-sequence-id-345244

    Enlarge

  21. pGBW-m4046858

    Plasmid
    #145658
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145658-plasmid-map-sequence-id-287835

    Enlarge

  22. pGBW-m4046730

    Plasmid
    #145659
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145659-plasmid-map-sequence-id-287836

    Enlarge

  23. pGBW-m4046823

    Plasmid
    #145634
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;Myc
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145634-plasmid-map-sequence-id-287811

    Enlarge

  24. pGBW-m4046816

    Plasmid
    #145622
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145622-plasmid-map-sequence-id-287799

    Enlarge

  25. pGBW-m4046791

    Plasmid
    #145586
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145586-plasmid-map-sequence-id-287763

    Enlarge

  26. pGBW-m4046774

    Plasmid
    #145574
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145574-plasmid-map-sequence-id-287751

    Enlarge

  27. pGBW-m4046846

    Plasmid
    #145575
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145575-plasmid-map-sequence-id-287752

    Enlarge

  28. Ec FLAG-dnaB-pET

    Plasmid
    #241267
    Purpose
    Over-express Ec FLAG-dnaB subunit in E. coli
    Depositor
    Michael O'Donnell
    Insert
    FLAG-dnaB
    Use
    Tags
    3X FLAG
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    241267-plasmid-map-sequence-id-482110

    Enlarge

  29. pRS415-GPD-PPK

    Plasmid
    #183941
    Purpose
    Expresses E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK (ppk E. coli)
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183941-plasmid-map-sequence-id-363309

    Enlarge

  30. HP17deltaP8

    Plasmid
    #235447
    Purpose
    Helper plasmid for regulated phage transfer
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    Coding part of the M13KO7 helper phage genome, except gene VIII
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    235447-plasmid-map-sequence-id-469112

    Enlarge

  46. pSMART-2,3-BDO1

    Plasmid
    #65816
    Purpose
    NADH Dependent 2,3-BDO Production Construct, Low Phosphate Dependent E. coli Expression
    Depositor
    Michael Lynch
    Inserts
    budA: α-acetolactate decarboxylase
    budB: acetolactate synthase
    budC: acetoin reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    waaHp from E. coli as part of operon with budA an…
    Available since
    July 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    65816-plasmid-map-sequence-id-111948

    Enlarge

  49. pHK401

    Plasmid
    #235482
    Purpose
    Repression of gene VIII by Plux/tet hybrid promoter
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    phage M13 geneVIII, cI repressor
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    235482-plasmid-map-sequence-id-473111

    Enlarge

  50. pHK201

    Plasmid
    #235481
    Purpose
    Repression of gene VIII by Ptac promoter
    Depositor
    Yolanda Schaerli
    Article
    et al Nat Commun 16, 3569 (2025)
    Insert
    phage M13 geneVIII, cI repressor
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    235481-plasmid-map-sequence-id-472224

    Enlarge

Showing: 301 - 350 of 9389 results