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Plasmid#148319PurposeMammalian Expression of HsYTHDF2DepositorInsertHsYTHDF2 (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1_AA
Plasmid#148246PurposeMammalian Expression vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-2xMS2-lacO
Plasmid#181909PurposeSingle guide RNA with 2XMS2 loops targeting bacterial lac operatorDepositorInsertsgRNA-2XMS2-lacO
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-2xMS2-mSAT
Plasmid#181908PurposeSingle guide RNA with 2XMS2 loops targeting mouse major satellite repeatsDepositorInsertsgRNA-2XMS2-mSAT
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-DmRoquin_AD
Plasmid#148531PurposeMammalian Expression of DmRoquinDepositorInsertDmRoquin (roq Fly)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_MCP-VP64_D-MS2
Plasmid#177161PurposeExpresses transactivator (MCP-VP64) and scRNA (D-MS2) in yeast cellsDepositorInsertsMCP-VP64
D-MS2
UseTagsSV40 NLSExpressionYeastMutationPromoterADH1 promoter and SNR52 promoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS42K_MCP-VP64_C-MS2
Plasmid#177160PurposeExpresses transactivator (MCP-VP64) and scRNA (C-MS2) in yeast cellsDepositorInsertsMCP-VP64
C-MS2
UseTagsSV40 NLSExpressionYeastMutationPromoterADH1 promoter and SNR52 promoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-sgRNA-MS2-U6
Plasmid#171694PurposeTo construct expression vector for tBE-V5-mA3DepositorInsertsgRNA scaffold-MS2-U6
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2592.EF1a.SLC6A1.S295L.RFP.P2A.PURO.JL.p163
Plasmid#170174PurposeLentiviral Expression of EF1a.SLC6A1.S295L.RFPDepositorInsertSLC6A1 (SLC6A1 Human)
UseLentiviralTagsExpressionMutationS295LPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2592.EF1a.SLC6A1.A288V.P2A.PURO.JL.p166
Plasmid#170171PurposeLentiviral Expression of EF1a.SLC6A1.A288VDepositorInsertSLC6A1 (SLC6A1 Human)
UseLentiviralTagsExpressionMutationA288VPromoterAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2)_Prnp_SAM2
Plasmid#78625PurposeEncodes sgRNA2.0 targeting 138nt upstream of TSSDepositorInsertsgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2)_Prnp_SAM1
Plasmid#78624PurposeEncodes sgRNA2.0 targeting 158nt upstream of TSSDepositorInsertsgRNA(MS2)_Prnp_SAM2 (Prnp Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (5'GAC)
Plasmid#170127PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(5'GAC)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
MS2-adRNA (3'GAC)
Plasmid#170129PurposeLentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125DepositorInsertMS2-ADAR2(3'GAC)-MS2
UseLentiviralTagsExpressionMammalianMutationPromoterHuman U6 and mouse U6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
lenti MS2-P65-HSF1_Hygro
Plasmid#61426Purposelenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2DepositorInsertMS2-P65-HSF1_2A_Hygro (HSF1 Human, Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NES
Plasmid#124703PurposeExpresses MS2_adRNA targeting the RAB7A locus along with MCP_ADAR1 DD (E1008Q)_NESDepositorInsertMCP_ADAR2 DD (E1008Q)_NES
UseAAVTagsNESExpressionMammalianMutationE1008Q hyperactive mutantPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR2 DD (E488Q)_NES
Plasmid#124707PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NESDepositorInsertMCP_ADAR2 DD (E488Q)_NES
UseAAVTagsNESExpressionMammalianMutationE488Q hyperactive mutantPromoterCMVAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-Tre3G-47xCAG-12xMS2
Plasmid#99148PurposeEncodes 47xCAG repeats with 12xMS2 tag, 2nd generation lentiviral vectorDepositorInsert47xCAG 12xMS2 hairpins
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-Tre3G-29xGGGGCC-12xMS2
Plasmid#99149PurposeEncodes 29xGGGGCC repeats with 12xMS2 tag, 2nd generation lentiviral vectorDepositorInsert29xGGGGCC 12xMS2 hairpins
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFH2.32_LV-mCherry-MS2x8-BCS
Plasmid#205539PurposeExpress mCherry-MS2x8 with barcodeDepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgRNA(MS2) cloning backbone
Plasmid#61424PurposesgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-2NLS-Cas9-6XMS2
Plasmid#166033PurposeExpression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti sgRNA(MS2) mCherry
Plasmid#171053PurposeExpression of Vp64-dCas9 activation systemDepositorInsertsgRNA(MS2) mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a MS2-Fkbp2x 2A Hygro
Plasmid#102807PurposeLentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp2x fusion proteinDepositorInsertMS2-Fkbp2x
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
(attB) BRaf with MS2-circRNA
Plasmid#226164PurposeUsed to integrate HA-tagged BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell linesDepositorInsertsUseTags2xHAExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-SSAP-MS2-BB_BbsI
Plasmid#183826PurposepU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFPDepositorInsertU6-guideRNA-dCas9-T2A-EBFP
UseCRISPRTagsExpressionMammalianMutationPromoterCBHAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-bActinCDS-bActinUTR-MS2V5
Plasmid#102718PurposeHalo-Actin reporter for detection of both mRNA and proteinDepositorInsertHaloTag-bActinCDS-bActin3'UTR-MS2V5
UseLentiviralTagsHaloTag and MS2V5ExpressionMutationPromoterUbCAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
phage-cmv-cfp-24xms2
Plasmid#40651DepositorInsertCFP-24xMBS
UseLentiviralTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs
Plasmid#86828PurposeExpression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequenceDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-Hs4E-T_AA
Plasmid#148233PurposeMammalian Expression of Hs4E-TDepositorInsertHs4E-T (EIF4ENIF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2CP-VPg(FCV)
Plasmid#167314PurposeTemplate pDNA to prepare CaVT mRNADepositorInsertCaVT
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-RhoBAST-MS2
Plasmid#196342PurposeOverexpression of circular RhoBAST-MS2DepositorInsertTornado-RhoBAST-MS2
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI-MS2V5-eRF3a F76a
Plasmid#65811Purposeexpression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1DepositorInserteRF3a (GSPT1 Human)
UseTagsMS2 stem loops and V5ExpressionMammalianMutationF76aPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
TetO7-HSP30UTR-nLucPEST-MS2(v4)
Plasmid#199764PurposeControl construct of elongation reporter to quantify elongation of LacZDepositorInsertNanolucPEST
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLH-sgRNA-Sirius-8XMS2
Plasmid#121939PurposeCRISPR-Sirius plasmidDepositorInsertsgRNA-Sirius-8XMS2
UseCRISPR and LentiviralTagsnoExpressionMammalianMutationPromoterhuman U6 promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmax pona 12xTRICK 24xMS2SL
Plasmid#64542PurposeTRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.DepositorInsert12x PP7 stem-loops
UseTags24xMS2 stem loops, HA, NLS, and dNP799ExpressionMammalianMutationPromoterponAAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsStaufen2-T1_V
Plasmid#147741PurposeMammalian Expression of HsStaufen2-T1DepositorInsertHsStaufen2-T1 (STAU2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
sg2.02 16x(MS2) MUC4.1
Plasmid#101154PurposegRNA with 16 MCP binding siteDepositorInsertMUC4 (MUC4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSaCas9-1xms2-2x3’UTR
Plasmid#122946PurposeFor expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTRDepositorInsertSaCas9-MS2-HBB 3' UTR
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMDlg/pRRE-int-MS2M
Plasmid#166032PurposeExpression of bacteriophage-derived MS2 coat protein fused HIV-1 Lentiviral Gag-Pol at C terminal with a HIV-1 protease cleavage signal between MS2 and Gag-Pol.DepositorInsertGag-Pol-MS2
UseLentiviralTagsExpressionMutationD64V mutation within the integrase within PolPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPA2-B1_Z
Plasmid#148113PurposeMammalian Expression of HshnRNPA2-B1DepositorInsertHshnRNPA2-B1 (HNRNPA2B1 Human)
UseTagsExpressionMammalianMutationsplicing variant of hnRNPA2 variant B1. Exon 6 an…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD
Plasmid#168153PurposeInducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.DepositorInsertsPmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS
Plasmid#124705PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLSDepositorInsertMCP_ADAR2 DD (E488Q)_NLS
UseAAVTagsNLSExpressionMammalianMutationE488Q hyperactive mutantPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
psi-CHECK3-3xBoxB-MS2
Plasmid#136011Purpose3 repeats of BoxB and MS2 hairpins inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid for the Tethering assayDepositorInsertNo gene (Artificial)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NLS
Plasmid#124626PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD(E1008Q)_NLSDepositorInsertMCP_ADAR1 DD (E1008Q)_NLS
UseAAVTagsNLSExpressionMammalianMutationE1008Q hyperactive mutantPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMS2CP-myc-T2A-tagRFP
Plasmid#140259PurposeExpresses WT MS2CP in mammalian cellsDepositorInsertCoat protein of bacteriphage MS2
UseSynthetic BiologyTagsmyc-T2A-tagRFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xMS2_SL]
Plasmid#68426PurposeTransient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing three MS2 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-MS2V5-PABPC1 MLLEmut
Plasmid#65808Purposeexpression of PABPC1-MS2 fusion. Mutation of C-terminal MLLE domain.DepositorInsertPABPC1 (PABPC2 Human)
UseTagsMS2 stem loops and V5ExpressionMammalianMutationMLLEmutPromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV Fos YFP MS2
Plasmid#131775PurposeFos upstream msfYFP 24xMS2 Fos downstream for CRISPR taggingDepositorInsertmsfYFP - 24x MS2 loops
UseCRISPRTagsExpressionMutationPromotern/aAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1
Plasmid#176839PurposeTo recruit SAM complex to human CYP3A4 proximal enhancer.DepositorInsertHuman CYP3A4 proximal enhancer sgRNA for activation
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only