We narrowed to 6,626 results for: GFP expression plasmids
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Plasmid#112952PurposeRhoA FRET biosensorDepositorInsertRhoA sensor
TagsLSS-SGFP2 and mScarlet-IExpressionMammalianPromoterCMVAvailable SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
RhoA sensor (LSS-SGFP2-Scarlet-I)
Plasmid#112947PurposeRhoA FRET biosensorDepositorInsertRhoA sensor
TagsLSS-SGFP2 and mScarlet-IExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]
Plasmid#86454PurposeNuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
ExpressionInsectAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUS252b
Plasmid#191831PurposeExpresses fuGFPb constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPb to place in other plasmid backbonesDepositorInsertfuGFPb
ExpressionBacterialAvailable SinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUS252y
Plasmid#191834PurposeExpresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.DepositorInsertfuYFP
ExpressionBacterialAvailable SinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUS252s
Plasmid#191833PurposeExpresses fuGFPs constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPs to place in other plasmid backbonesDepositorInsertfuGFPs
ExpressionBacterialAvailable SinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOD2049-csnControl
Plasmid#100007PurposeMosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2048-csnDEG. Expression is controlled by Posm-6 (ciliated sensory neuron specific).DepositorInsertsUseTargeting vector for mos1 transposon mediated sin…Available SinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLexM-HsPQLC1(16-271)-TEV-GFP-His8
Plasmid#250419PurposeExpression of C-terminally TEV-GFP-His8 Tagged, N-Terminally Truncated (?1-15), HsPQLC1 in Mammalian CellsDepositorInsertPQLC1 (SLC66A2 Human)
TagsTEV-GFP-8xHisExpressionMammalianMutationΔ1-15 (new Start codon added)PromoterChicken-beta-actin promoter/CMV EnhancerAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLexM-HsPQLC1(20-271)-TEV-GFP-His8
Plasmid#250420PurposeExpression of C-terminally TEV-GFP-His8 Tagged, N-Terminally Truncated (?1-19), HsPQLC1 in Mammalian CellsDepositorInsertPQLC1 (SLC66A2 Human)
TagsTEV-GFP-8xHisExpressionMammalianMutationΔ1-19 (new Start codon added)PromoterChicken-beta-actin promoter/CMV EnhancerAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLexM-HsPQLC1(24-271)-TEV-GFP-His8
Plasmid#250421PurposeExpression of C-terminally TEV-GFP-His8 Tagged, N-Terminally Truncated (?1-23), HsPQLC1 in Mammalian CellsDepositorInsertPQLC1 (SLC66A2 Human)
TagsTEV-GFP-8xHisExpressionMammalianMutationΔ1-23 (new Start codon added)PromoterChicken-beta-actin promoter/CMV EnhancerAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pSB_init-Sb44
Plasmid#159421PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb44DepositorInsertSb44
Tagsc-Myc and poly-HisExpressionBacterialAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-nTermPuroR-nTermIntein
Plasmid#208378PurposeEpisomal plasmid - for expression of 2 proteins linked with EGFP and n-terminal split puromycin resistance and split inteinDepositorTypeEmpty backboneExpressionMammalianPromoterhuman CMVAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
MigR1 GFP Mouse PHGDH
Plasmid#220591PurposeRetroviral mammalian expression vector with GFP for overexpression of mouse PHGDHDepositorInsertMs PHGDH (Phgdh Mouse)
UseRetroviralAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)
Plasmid#223113PurposepCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFPDepositorInserthuman codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
UseCRISPRTagsBPNLS-P2A-EGFP and BPNLS-TadA8eExpressionMammalianMutationTadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…PromoterCMV and T7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2
Plasmid#196250PurposePlasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119DepositorInsertattL1-GlpT-scGFP-attL2
ExpressionBacterialPromoterJ23119 promoterAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-SpCas9-NRRH-P2A-EGFP (RMD47)
Plasmid#197499PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRRH-BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationABE8.20 mutations in TadA and nSpCas9-NRRH(D10A/I…PromoterCMV and T7Available SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)
Plasmid#197500PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…PromoterCMV and T7Available SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA
Plasmid#194247PurposeExpresses 3 miRNAs targeting human alpha-SynucleinDepositorAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-Gluc-RMA-IRES-EGFP
Plasmid#189630PurposeExpresses Gluc-RMA and EGFP in the presence of Cre recombinase. Double-floxed Gluc-RMA and EGFP for monitoring Cre-expressing neuronal populations.DepositorInsertsGaussia luciferase fused to Fc
IRES-EGFP
UseAAV, Cre/Lox, and LuciferaseTagsGluc and IgG1 FcExpressionMammalianPromoterIRES and hSynAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP
Plasmid#176279PurposeViral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.DepositorInsertKir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
UseAAV and Cre/LoxTagsMycExpressionMammalianMutationGYG to AAA (aa144-146)Promoterhuman Synapsin IAvailable SinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP
Plasmid#113158PurposeAn AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporterDepositorInsertgRNA for rat TH
UseAAV, CRISPR, and Cre/LoxExpressionMammalianPromotermU6Available SinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
1530_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-EmGFP-spA
Plasmid#109317PurposePlasmid for liver-specific expression of EmGFP with a BbsI cloning site for a gRNADepositorTypeEmpty backboneUseAAV and CRISPRExpressionMammalianAvailable SinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP(L1939A,L1942A)-GFP-shch-TOG
Plasmid#69114PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG with L1939A and L1942A substitutions tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationL1939A and L1942A amino acid substitutions. Sile…PromoterCMVAvailable SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-PiGM-Iq(eGFP)
Plasmid#221602PurposeA recombinant AAV2 plasmid encoding the PiGM-Iq system with CRY2PHR, CIBN and EGFP as expression marker. hSynapsin promoter for panneuronal expression.DepositorInsertPiGM-Iq (eGFP)
UseAAVMutationRGS2 1-53 truncationPromoterHuman SynapsinAvailable SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-2xMARS-nGFP-puro
Plasmid#205238PurposeLentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobodyDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseLentiviralTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable SinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAD-5PSD95-EGFP-SynPhRFP
Plasmid#217979PurposeG-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFPDepositorUseG-deleted rabiesTagsEGFP and RFPPromoterNoneAvailable SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV2_hSyn_NES-Caprola_off-mEGFP_WRPE-SV40
Plasmid#194692PurposehSyn1 driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through AAV transductionDepositorInsertCaprola_off-mEGFP
UseAAVTagsmEGFPPromoterhSyn1Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID-GFP
Plasmid#209401PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
TagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-GOI-DE-TurboID-GFP
Plasmid#209389PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneTagsGFPExpressionPlantMutationTurboID gene sequence C249AAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Blast-H2BC11
Plasmid#207757PurposeDonor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
AAV-sCAG-eGFP-ACAP1
Plasmid#203800PurposeAAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoterDepositorAvailable SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP[D134TAG+N150TAA]
Plasmid#164582Purposesuperfolder GFP with D134TAG and N150TAA mutationsDepositorInsertsfGFP-134TAG+150TAA
Tags6xHisExpressionBacterialMutationD134TAG + N150TAA MutationsPromoterT7Available SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot7-D40AE42A_L
Plasmid#146890PurposeMammalian Expression of HsNot7-D40AE42A. Please note that this plasmid does not contain the T7 promoter.DepositorInsertHsNot7-D40AE42A (CNOT7 Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-GPXC71S
Plasmid#191760PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758DepositorInsertnuc-roGFP2-GPXC71S
UseBinary agrobacterium ti plasmidExpressionPlantMutationGPX has no peroxidatic Cysteine 71, which is repl…PromoterCaMV 35SAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mCherry-ApoB-TagBFP
Plasmid#129383PurposePlasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFPDepositorInsertmCherry-ApoB-Tag-BFP
ExpressionMammalianPromoterCMV promoterAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPLN-hFluc-WT-HA-GFP11-KDEL
Plasmid#177722PurposeFirefly luciferase reporter fused to a prolactin signal sequence for ER targeting and KDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminusDepositorInserthFluc
TagsGFP11, HA, KDEL, and Prolactin Signal SequenceExpressionBacterial and MammalianAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHD118-GFP-HiBit
Plasmid#171015PurposeFor bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHD118-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-pHlip-GFP-HiBit
Plasmid#171016PurposeFor bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-pHlip-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-hSncg-EGFP
Plasmid#153198PurposeHuman gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVExpressionMammalianAvailable SinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S(rc)2_4_5-GFP
Plasmid#127520PurposePlasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.DepositorInsertCaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-T2A-LSS-SGFP2
Plasmid#112944PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmCherry and LSS-SGFP2
ExpressionMammalianPromoterCMVAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG-RORb2-IRES-GFPd2
Plasmid#73998PurposePlasmid expressing RORb2-IRES-GFPd2DepositorAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS
Plasmid#85821PurposeiDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertZf Ubi::cMyc oDam_f_GFPoNLS
UseTol2 based vectorTagscMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…MutationL122A. Codon optimization compatible to work in M…Available SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHIS3p:CloverGFP-Tub1+3'UTR::TRP1
Plasmid#50648DepositorInsertCloverGFP-Tub1
UseYeast expressionTagsCloverGFPPromoterHIS3pAvailable SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
SREC 2: abu-1::abu-1::gfp
Plasmid#21825DepositorAvailable SinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLexM-HsPQLC1(Δ100-125/GGGS)-TEV-GFP-His8
Plasmid#250426PurposeExpression of C-terminally TEV-GFP-His8 Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a GGGS Linker) in Mammalian CellsDepositorInsertPQLC1 (SLC66A2 Human)
TagsTEV-GFP-8xHisExpressionMammalianMutationΔ100-125 (Replaced with GGGS)PromoterChicken-beta-actin promoter/CMV EnhancerAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLexM-HsPQLC1(Δ100-125/3xGGGS)-TEV-GFP-His8
Plasmid#250428PurposeExpression of C-terminally TEV-GFP-His8 Tagged HsPQLC1 (Δ100-125 - with these residues replaced by a 3xGGGS Linker) in Mammalian CellsDepositorInsertPQLC1 (SLC66A2 Human)
TagsTEV-GFP-8xHisExpressionMammalianMutationΔ100-125 (Replaced with 3xGGGS)PromoterChicken-beta-actin promoter/CMV EnhancerAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Fis1-DTM
Plasmid#191950PurposeExpression of a Fis1-GFP chimera lacking the C-terminal 27 residues (Fis1-DTM)DepositorInsertFis1-DTM
ExpressionBacterialMutationFis1 gene lacking the C-terminal 27 residuesAvailable SinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUSE-URA3-Sec7-msYFP2
Plasmid#218969PurposeGene replacement plasmid to label S. cerevisiae Sec7 with msYFP2DepositorAvailable SinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-eGFP-hTau S262D-WPRE-pA
Plasmid#255026PurposeLentiviral plasmid designed to express a fusion protein of enhanced GFP and human Tau containing a S262D mutationDepositorAvailable SinceMay 14, 2026AvailabilityAcademic Institutions and Nonprofits only