We narrowed to 11,913 results for: pcDNA

1. pY012 (pcDNA3.1-hCMtCpf1)

   Plasmid

   #69984

   Purpose

   Expresses humanized CMtCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hCMtCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA5/FRT/TO_Lifeact_STf

   Plasmid

   #182011

   Purpose

   CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   Lifeact_STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDNA3-V5-PTPN14-del-N

   Plasmid

   #61004

   Purpose

   Expresses human PTPN14 (del-FERM domain mutant) in mammalian cells, N-terminus V5 tag

   Depositor

   Jianmin Zhang

   Article

   Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

   Insert

   PTPN14 delete N (PTPN14 Human)
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   deleted amino acids 17-310

   Promoter

   CMV

   Available since

   Nov. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

4. Rat NCX1.7 (pcDNA3.1+)

   Plasmid

   #75192

   Purpose

   Mammalian expression of SLC8A1

   Depositor

   Jonathan Lytton

   Article

   Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

   Insert

   SLC8A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA5-FRT-TO-Nsp7

   Plasmid

   #157688

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp7 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pcDNA3-mCherry-LKB1(KD)

   Plasmid

   #84640

   Purpose

   mCherry-tagged, LKB1(K78I); kinase-dead mutant

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   mCherry-LBK1(K78I) (STK11 Synthetic, Human)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   Lysine 78 mutated to Isoleucine; kinase dead

   Promoter

   CMV

   Available since

   May 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-HA-hTRAF3 1-448

   Plasmid

   #44033

   Depositor

   Shao-Cong Sun

   Article

   Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

   Insert

   TNF receptor-associated factor 3 (TRAF3 Human)
   

   Use

   Tags

   1xHA

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-448; V274D and S393G

   Promoter

   Available since

   April 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Rs4084-RBD-8h

   Plasmid

   #161825

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4084

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T321-D519)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-Rs4231-RBD-8h

   Plasmid

   #161824

   Purpose

   Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4231

   Depositor

   Erik Procko

   Article

   Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

   Insert

   Receptor-binding domain (RBD) of spike protein S
   

   Use

   Tags

   GSG linker and 8his tag and HA leader sequence

   Expression

   Mammalian

   Mutation

   Human codon optimized RBD (a.a. T320-D518)

   Promoter

   CMV

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3 Flag MKK7b1

    Plasmid

    #14622

    Depositor

    Roger Davis

    Article

    Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

    Insert

    MKK7b1 (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-GFPLaminA-R482W

    Plasmid

    #32709

    Depositor

    Wendy Bickmore

    Article

    Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

    Insert

    Lamin A (LMNA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    R482W

    Promoter

    CMV

    Available since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

12. mcherry Gamma 9 ---pcDNA3.1

    Plasmid

    #64203

    Purpose

    G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

    Depositor

    Narasimhan Gautam

    Article

    Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    G protein Gamma 9 (GNGT2 Human)
    

    Use

    Tags

    6xHis and mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5-FRT-TO-FH-Nsp2

    Plasmid

    #157683

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp2 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pcDNA3-HCMVgH (Merlin)

    Plasmid

    #136448

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain), full-length

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA6-C-3XFLAG-h-Lmbr1l

    Plasmid

    #123462

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    Limb development membrane protein 1 like (LMBR1L Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hSlo2.1 Slick/pCDNA3

    Plasmid

    #197317

    Purpose

    Plasmid expressing an antigen used to generate an antibody that targets the Slo2.1 Slick K+ channel subunit

    Depositor

    Christopher Lingle

    Insert

    Slo2.1 (Kcnt2 Mouse)
    

    Use

    Tags

    7xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5FRT/TO-PNGase-Strep-HA

    Plasmid

    #113490

    Purpose

    Expression of human PNGase with C-terminal strep-HA tag

    Depositor

    Hemmo Meyer

    Article

    Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

    Insert

    PNGase (NGLY1 Human)
    

    Use

    Tags

    2xstrep-HA

    Expression

    Mammalian

    Mutation

    wildtype without stop codon

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO HSPH3

    Plasmid

    #19455

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 4-like (HSPA4L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA4.TO-ORF33-2xCSTREP

    Plasmid

    #136194

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF33

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF33
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA4.TO-ORF60-2xCSTREP

    Plasmid

    #136220

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF60

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF60
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCDNA4.TO-ORF38-2xCSTREP

    Plasmid

    #136199

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF38

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF38
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pF153 pcDNA3.1(+)SOD1G37R

    Plasmid

    #26399

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G37R

    Promoter

    Available since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

23. pCDNA3-V5-PTPN14-del-C

    Plasmid

    #61005

    Purpose

    Expresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tag

    Depositor

    Jianmin Zhang

    Article

    Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

    Insert

    PTPN14 delete C (PTPN14 Human)
    

    Use

    Tags

    V5 tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 934-1187

    Promoter

    CMV

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pCDNA3-hTICAM1-VenusFlag

    Plasmid

    #197632

    Purpose

    Human TRIF protein expression in mammalian cells

    Depositor

    Haian Fu

    Insert

    TICAM1 (TICAM1 Human)
    

    Use

    Tags

    Venus-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA-Flag-LmoCas8b2

    Plasmid

    #126482

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas8b2
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-Flag-LmoCas7

    Plasmid

    #126483

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas7
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA-Flag-LmoCas5

    Plasmid

    #126484

    Purpose

    Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    LmoCas5
    

    Use

    CRISPR

    Tags

    Flag, NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. HA-USPL1-C236A-pcDNA3.1

    Plasmid

    #85764

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236A

    Promoter

    CMV

    Available since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HA-USPL1-C236S-pcDNA3.1

    Plasmid

    #85763

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236S

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-pri-let-7d

    Plasmid

    #51379

    Depositor

    Narry Kim

    Article

    Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

    Insert

    pri-let-7d (MIRLET7D Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-ALK2 K235R

    Plasmid

    #80872

    Purpose

    mammalian expression of ALK2 K235R

    Depositor

    Aristidis Moustakas

    Article

    Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

    Insert

    ALK2 (ACVR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K235R

    Promoter

    CMV

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3.1(+)Gαi1-91-NLuc

    Plasmid

    #207148

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Tom Van Loy, Dominique Schols

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)K

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA6-N-3XFLAG-Axin1

    Plasmid

    #123463

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    Axin 1 (Axin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDNA4.TO-ORF37-2xCSTREP

    Plasmid

    #136198

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF37

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF37
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pcDNA3.1-Renilla-ccdB

    Plasmid

    #87068

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal Renilla luciferase

    Depositor

    Mikko Taipale

    Type

    Empty backbone

    Use

    Tags

    Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pY017 (pcDNA3.1-hPcCpf1)

    Plasmid

    #69989

    Purpose

    Expresses humanized PcCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hPcCpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-FLAG-mTUT7

    Plasmid

    #60044

    Purpose

    Mammalian expression of FLAG-tagged mouse TUT7

    Depositor

    Zissimos Mourelatos

    Article

    Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

    Insert

    TUT7 (Zcchc6 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA5-FRT-TO-mCherry-N

    Plasmid

    #210271

    Purpose

    N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PCDNA3-N3FLAG-MYB-FL

    Plasmid

    #105609

    Purpose

    transient express full length MYB with 3*FLAG tag at N terminal in HEK 293T cells

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    Full length MYB (mouse) (Myb Mouse)
    

    Use

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5/FRT/TO HSPH1

    Plasmid

    #19453

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    HSPH1 (HSPH1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge