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Plasmid#131167PurposePCR template for genomic C-terminal Cub-R-URA3 tag, URA3 complements the uracil auxotrophy of S. cerevisiae ura mutantDepositorTypeEmpty backboneUseTagsCCGExpressionYeastMutationPromoterAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only
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25Q-ymsfGFP
Plasmid#128559PurposeHttex1 (without proline-rich region) fused to ymsfGFPDepositorInsert25Q-Httex1 (without proline-rich region) fused to ymsfGFP
UseTagsymsfGFPExpressionYeastMutationPromoterGal1Available sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
68Q-ymScarlet
Plasmid#128562PurposeHttex1 (without proline-rich region) fused to ymScarletDepositorInsert68Q-Httex1 (without proline-rich region) fused to ymSarlet
UseTagsymScarletExpressionYeastMutationPromoterGal1Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
25Q-ymScarlet
Plasmid#128561PurposeHttex1 (without proline-rich region) fused to ymScarletDepositorInsert25Q-Httex1 (without proline-rich region) fused to ymSarlet
UseTagsymScarletExpressionYeastMutationPromoterGAL1Available sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCuHA-ATG13[3SD](426)
Plasmid#122077PurposeExpressing HA-ATG13[3SD]DepositorInsertATG13
UseTags3HAExpressionYeastMutationSerine 646 to aspartic acid; Serine 649 to aspart…PromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM002
Plasmid#122936PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML043C (Marked)
UseTagsExpressionYeastMutationYML043C: bp 52-63 AAGTTAAAGTAT to AAATTGAAATACPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM001
Plasmid#122935PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML029W (Marked)
UseTagsExpressionYeastMutationYML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTAPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEC005
Plasmid#122939PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML071C (Marked)
UseTagsExpressionYeastMutationYML071C: bp 52-63 AAACGGTTAAGT to AAGCGCTTGAGCPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_207
Plasmid#122938PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML070W (Marked)
UseTagsExpressionYeastMutationYML070W: bp 52-63 GGGTTTGCCCTT to GGCTTCGCGCTAPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_243
Plasmid#122940PurposeExpression of the marked mRNA under the control of insertional promoters.DepositorInsertYML029W (Marked)
UseTagsExpressionYeastMutationYML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTAPromoterPyml029w-tetO(2)Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM004
Plasmid#122937PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML065W (Marked)
UseTagsExpressionYeastMutationYML065W: bp 52-63 GAGCAGGGAAAT to GAACAAGGTAACPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_228
Plasmid#122933PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYBR025C (Marked)
UseTagsExpressionYeastMutationYBR025C: bp 52-63 GGTAATAACTTG to GGAAACAATTTAPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_229
Plasmid#122934PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYBR026C (Marked)
UseTagsExpressionYeastMutationYBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAGPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATG13[3SD](315)
Plasmid#122074PurposeExpressing GFP-Atg13[3SD]DepositorInsertATG13
UseTagsGFPExpressionYeastMutationSerine 646 to aspartic acid; Serine 649 to aspart…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP-ATG13[3SA](315)
Plasmid#122073PurposeExpressing GFP-Atg13[3SA]DepositorInsertATG13
UseTagsGFPExpressionYeastMutationSerine 646 to alanine; Serine 649 to alanine; Ser…PromoterAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTD3685
Plasmid#113391Purposealpha factor inducible Ty3DepositorInsertTy3
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAEL012356 AstC R
Plasmid#115401PurposeExpresses Ae. aegypti receptor in mammalian cellsDepositorInsertAAEL012356 AstC R (LOC5576162 Aedes aegypti)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB2629
Plasmid#113394PurposeMET25p-MS2-RFP-CYC1 TTDepositorInsertMS2 bacteriophage coat protein
UseTagsred fluorescent protein (RFP), codon-optimized fo…ExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVEG16*
Plasmid#111855PurposeXylose-responsive Gal3p-Syn4.1 under control of native GAL3p (promoter).DepositorInsertGAL3p-GAL3-Syn4.1-TEFt
UseSynthetic BiologyTagsExpressionYeastMutationD68N, V69M, A109V, I271LPromoterGAL3Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K818R
Plasmid#111956PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K818R mutationDepositorInsertHsh155 K818R
UseTagsExpressionBacterial and YeastMutationK818RPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 V783L
Plasmid#111955PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the V783L mutationDepositorInsertHsh155 V783L
UseTagsExpressionBacterial and YeastMutationV783LPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 N747D
Plasmid#111954PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the N747D mutationDepositorInsertHsh155 N747D
UseTagsExpressionBacterial and YeastMutationN747DPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 3xFLAG
Plasmid#111960PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has 3xFLAG at the C-terminus.DepositorInsertHsh155 3x FLAG
UseTags3xFLAGExpressionBacterial and YeastMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Y826A
Plasmid#111952PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Y826A mutationDepositorInsertHsh155 Y826A
UseTagsExpressionBacterial and YeastMutationY826APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 V783A
Plasmid#111950PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the V783A mutationDepositorInsertHsh155 V783A
UseTagsExpressionBacterial and YeastMutationV783APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 N747A
Plasmid#111949PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the N747A mutationDepositorInsertHsh155 N747A
UseTagsExpressionBacterial and YeastMutationN747APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R744A
Plasmid#111948PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the R744A mutationDepositorInsertHsh155 R744A
UseTagsExpressionBacterial and YeastMutationR744APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Q776A
Plasmid#111944PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q776A mutationDepositorInsertHsh155 Q776A
UseTagsExpressionBacterial and YeastMutationQ776APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R775A
Plasmid#111943PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the R775A mutationDepositorInsertHsh155 R775A
UseTagsExpressionBacterial and YeastMutationR775APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 Q773A
Plasmid#111942PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A mutationDepositorInsertHsh155 Q773A
UseTagsExpressionBacterial and YeastMutationQ773APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R186A
Plasmid#111939PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the R186A mutationDepositorInsertHsh155 R186A
UseTagsExpressionBacterial and YeastMutationR186APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R182A
Plasmid#111938PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the R182A mutationDepositorInsertHsh155 R182A
UseTagsExpressionBacterial and YeastMutationR182APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R181A
Plasmid#111937PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the R181A mutationDepositorInsertHsh155 R181A
UseTagsExpressionBacterial and YeastMutationR181APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 T178A
Plasmid#111936PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the T178A mutationDepositorInsertHsh155 T178A
UseTagsExpressionBacterial and YeastMutationT178APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K818A
Plasmid#111951PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K818A mutationDepositorInsertHsh155 K818A
UseTagsExpressionBacterial and YeastMutationK818APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K740A
Plasmid#111947PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K740A mutationDepositorInsertHsh155 K740A
UseTagsExpressionBacterial and YeastMutationK740APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 R778A
Plasmid#111945PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the R778A mutationDepositorInsertHsh155 R778A
UseTagsExpressionBacterial and YeastMutationR778APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K227A
Plasmid#111941PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K227A mutationDepositorInsertHsh155 K227A
UseTagsExpressionBacterial and YeastMutationK227APromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTCW010
Plasmid#83521PurposeAromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha2 geneDepositorInsertpARO9-mfalpha2
UseTagsExpressionYeastMutationPromoterpARO9Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW009
Plasmid#83523PurposeAromatic amino acid inducible expression of alpha pheromone in yeast from the mfalpha1 geneDepositorInsertpARO9-mfalpha1
UseTagsExpressionYeastMutationPromoterpARO9Available sinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PHBA01
Plasmid#83535PurposeConstitutive TEF1 promoter mediated expression of the UBiC geneDepositorInsertpTEF1-UBiC-CYC1t
UseTagsExpressionYeastMutationPromoterpTEF1Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTCW005
Plasmid#83524Purposepheromone mediated alpha pheromone expression in yeast from the mfalpha 1 geneDepositorInsertpFUS1-malpha1
UseTagsExpressionYeastMutationPromoterpFUS1Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
SepOTSι
Plasmid#68290PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EFSep21, 2xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSη
Plasmid#68288PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS9/EF-Sep21, 2xtRNA-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSζ
Plasmid#68287PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS/EF-Sep, 2xtRNA-Sep
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
SepOTSδ
Plasmid#68285PurposePhosphoserine orthogonal translation systemDepositorInsertSepRS/EF-Sep, 1xtRNA-Sep-A37
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP34
Plasmid#65501PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-t7TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP35
Plasmid#65502PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP36
Plasmid#65504PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFLIP42
Plasmid#65617PurposeFRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneUseTags6His-Cit-attR1 and attR2-mCer-cMycExpressionBacterialMutationPromoterT7 promoterAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only