Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 24,908 results for: Spr

Clear keywords
Showing: 3551 - 3600 of 24908 results

  2. pYPQ-dpcoCas9-Act3.0

    Plasmid
    #158408
    Purpose
    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:
    Insert
    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    158408-plasmid-map-sequence-id-330919

    Enlarge

  4. pQdCas12a.luxR(mut)-empty

    Plasmid
    #236037
    Purpose
    The plasmid pQdCas12a.luxR(mut)-empty expresses the dCas12a endonuclease. Additionally, it contains a mutation in the luxR gene.
    Depositor
    Radhakrishnan Mahadevan
    Article
    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-
    Insert
    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI, dcas12a, and lacZ-alpha (fragment).
    Use
    CRISPR
    Promoter
    quorum sensing promoter
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    236037-plasmid-map-sequence-id-469909

    Enlarge

  10. pACT2-CAS9c

    Plasmid
    #51055
    Purpose
    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.
    Depositor
    Yunde Zhao
    Article
    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1
    Insert
    Cas9c
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Bacterial and Yeast
    Promoter
    yeast ADH1 promoter
    Available Since
    Feb. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51055-plasmid-map-sequence-id-77327

    Enlarge

  11. pDM026

    Plasmid
    #216808
    Purpose
    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Type
    Empty backbone
    Use
    CRISPR; Fungal expression
    Expression
    Bacterial
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216808-plasmid-map-sequence-id-429385

    Enlarge

  14. pCRISPEY-GAL-Nat-ADE2

    Plasmid
    #232103
    Purpose
    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.
    Depositor
    Jeffrey Lewis
    Article
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    ADE2 gRNA and repair template
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Repair template introduces C at nt 466 of ADE2 to…
    Promoter
    GAL7
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232103-plasmid-map-sequence-id-461835

    Enlarge

  18. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

    Enlarge

  19. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172316
    Purpose
    CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172316-plasmid-map-sequence-id-343650

    Enlarge

  41. dpYPQ230-D156R-CBE1

    Plasmid
    #195367
    Purpose
    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1
    Insert
    hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
    Use
    CRISPR; Gateway compatible ha3a/y130f-xten plus 3…
    Tags
    3xflag
    Expression
    Plant
    Promoter
    ZmUBI1
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    195367-plasmid-map-sequence-id-392890

    Enlarge

  46. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129392-plasmid-map-sequence-id-266824

    Enlarge

Showing: 3551 - 3600 of 24908 results