166,169 results

1. pET His6 MBP TEV LIC cloning vector (1M)

   Plasmid

   #29656

   Depositor

   Scott Gradia

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   6xHis, MBP, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRX-IB-HaloTag7-mGFP

   Plasmid

   #184903

   Purpose

   Stable expression of HaloTag7-mGFP in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

   Insert

   monomeric EGFP
   

   Tags

   HaloTag7

   Expression

   Mammalian

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p16 nlsGPS2

   Plasmid

   #228093

   Purpose

   Expression plasmid for genetically-encoded FRET biosensor for Gibberellin (GA)

   Depositor

   Alexander Jones

   Article

   Griffiths et al Plant Cell. 2024 Jul 23:koae198. doi: 10

   Insert

   nlsGPS2
   

   Tags

   6xHis-T7 XpressTag and cMyc

   Expression

   Plant

   Promoter

   p16

   Available Since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiFUCCI(CA)5

   Plasmid

   #223176

   Purpose

   Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

   Depositor

   Neville Sanjana

   Article

   Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

   Insert

   FUCCI(CA)5
   

   Use

   Lentiviral

   Tags

   AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

   Expression

   Mammalian

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIF1079

   Plasmid

   #237445

   Purpose

   Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

   Depositor

   IIya Finkelstein

   Article

   Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/

   Inserts

   SpCas9
   Efe1-RT
   EMX1 sgRNA
   Efe1-ncRNA targeting EMX1
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CAG, H1, and U6

   Available Since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI pHluorin Syt12

   Plasmid

   #184513

   Purpose

   Mammalian expression of Synaptotagmin 12 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 12 (Syt12 Rat)
   

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIM1773-PRHIII-sfGFP-pNW33N

   Plasmid

   #52217

   Purpose

   Constitutive sfGFP expression plasmid for use in Geobacillus and E. coli

   Depositor

   Ichiro Matsumura

   Article

   Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

   Insert

   PRHIII-sfGFP
   

   Expression

   Bacterial

   Promoter

   PRHIII

   Available Since

   May 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT-CA-GFP-IRES-mLumin

   Plasmid

   #32749

   Depositor

   Jeanne Hardy

   Article

   Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

   Inserts

   CA-GFP
   mLumin
   

   Expression

   Mammalian

   Mutation

   S158A and S65T

   Promoter

   CMV

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCF1148

   Plasmid

   #222330

   Purpose

   Fluorescent reporter plasmid for Veillonella

   Depositor

   J Christopher Fenno

   Article

   Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

   Insert

   bs2 gene with Veillonella parvula SKV38 mdh promoter
   

   Use

   E. coli-veillonella shuttle plasmid

   Expression

   Bacterial

   Promoter

   Veillonella parvula SKV38 mdh promoter

   Available Since

   Oct. 31, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. LentiGuide Blast

    Plasmid

    #199622

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. tetO.Nfib.Hygro

    Plasmid

    #117271

    Purpose

    Expresses Nfib and hygromycin resistance gene under control of tetO sequence.

    Depositor

    Henrik Ahlenius

    Article

    Canals et al Nat Methods. 2018 Sep;15(9):693-696. doi

    Insert

    Nfib (Nfib Mouse)
    

    Use

    Lentiviral

    Tags

    IRES and hygromycin resistant cassette (C termina…

    Expression

    Mammalian

    Promoter

    tet-on

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-EGFP barcode-5-SV40 polyA

    Plasmid

    #190869

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode5
    

    Use

    AAV

    Mutation

    WT

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCF1135

    Plasmid

    #222106

    Purpose

    E. coli - Veillonella shuttle plasmid with single selectable marker

    Depositor

    J Christopher Fenno

    Article

    Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

    Type

    Empty backbone

    Use

    E. coli-veillonella shuttle plasmid

    Expression

    Bacterial

    Available Since

    Oct. 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid

    #101178

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

    Depositor

    Jennifer Doudna, Keith Joung

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/Q695A/H698A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PH-PLCD1-GFP

    Plasmid

    #51407

    Purpose

    Is a biosensor for PI(4,5)P2

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    PH-PLCD1 (aa 1–170) (PLCD1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNP3425 [pNP2922 - ISCro4 Enhanced bRNA (TBL4, WT + DBL3, WT) + ISCro4 Recombinase (WT)]

    Plasmid

    #247262

    Purpose

    Express ISCro4 recombinase and bridge RNA

    Depositor

    Patrick Hsu

    Article

    Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

    Insert

    pNP2922 - ISCro4 bRNA1-179 C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101-111) (TBL4+DBL3)
    

    Expression

    Mammalian

    Mutation

    C60U, A61G, U73C, dU86, A87G, dU88, ins179(rc(101…

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid

    #91796

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available Since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACYC-GroEL/ES-TF

    Plasmid

    #83923

    Purpose

    Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Gro EL/ES
    Trigger Factor
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRT043-CLYBL-DDdcas9-VPH

    Plasmid

    #158091

    Purpose

    Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

    Depositor

    Martin Kampmann

    Article

    Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

    Insert

    CLYBL-CAG-DDdCas9VPH-T2A-GFP
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-ABE8.8m-WT-P2A-EGFP (BKS953)

    Plasmid

    #242651

    Purpose

    CMV promoter expression plasmid for human codon optimized ABE8.8m A-to-G base editor with SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

    Insert

    pCMV-ABE8.8m-SpCas9-P2A-EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    ABE8.8 mutations in TadA

    Promoter

    CMV and T7

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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