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We narrowed to 7,473 results for: Lif;

Showing: 2921 - 2960 of 7473 results

  1. pUC19-π-EB1_IRES-mCherry

    Plasmid
    #197427
    Purpose
    Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    IRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    GCN4 leucine zipper, LOV2, Zdk1, and mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197427-plasmid-map-sequence-id-388174

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  2. pUC19-π-EB1_EF1α-mCherry

    Plasmid
    #197429
    Purpose
    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    GCN4 leucine zipper, LOV2, Zdk1, and mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197429-plasmid-map-sequence-id-388078

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  3. pUC19-π-EB1_EF1α-EGFP

    Plasmid
    #197430
    Purpose
    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    EGFP, GCN4 leucine zipper, LOV2, and Zdk1
    Expression
    Mutation
    Promoter
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197430-plasmid-map-sequence-id-388175

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  16. fru-T2A-QF2 HDR plasmid

    Plasmid
    #141099
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene
    Depositor
    Leslie Vosshall
    Article
    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10
    Insert
    fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
    Use
    Donor template
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    141099-plasmid-map-sequence-id-292289

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  17. pJET-62-SFFV-T2A-PuroR-polyA-R11b

    Plasmid
    #179893
    Purpose
    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out)
    Depositor
    Patrick Caswell
    Article
    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:
    Insert
    RAB11B, member RAS oncogene family (RAB11B Human)
    Use
    CRISPR; Donor for homologous based knock in
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179893-plasmid-map-sequence-id-357407

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  18. pJET-64-SFFV-T2A-BlaR-polyA-R11b

    Plasmid
    #179895
    Purpose
    Donor with homologous arms for Rab11b to knock in SFFV-T2A-Blasticidin resistance-PolyA cassette (for knock out)
    Depositor
    Patrick Caswell
    Article
    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:
    Insert
    RAB11B, member RAS oncogene family (RAB11B Human)
    Use
    CRISPR; Donor for homologous based knock in
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    SFFV
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179895-plasmid-map-sequence-id-357187

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  21. pCMV(MinDis)-iCyt_BrEt_SnFR_PM

    Plasmid
    #177747
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    pCMV(MinDis)-iCyt_BrEt_SnFR_PM
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177747-plasmid-map-sequence-id-349063

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  22. _pCMV(MinDis)-iCyt_F_SnFR_PM

    Plasmid
    #177745
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    _pCMV(MinDis)-iCyt_F_SnFR_PM
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177745-plasmid-map-sequence-id-349553

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  24. pCMV(MinDis)-iCyt_BrEt_SnFR_ER

    Plasmid
    #177746
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    pCMV(MinDis)-iCyt_BrEt_SnFR_ER
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177746-plasmid-map-sequence-id-349555

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  25. _pCMV(MinDis)-iCyt_F_SnFR_ER

    Plasmid
    #177744
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1
    Insert
    _pCMV(MinDis)-iCyt_F_SnFR_ER
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    177744-plasmid-map-sequence-id-349548

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  28. EGFP-uTEV1-MAVS-mTA6

    Plasmid
    #171004
    Purpose
    HiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria)
    Depositor
    Alice Ting
    Article
    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:
    Insert
    EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
    Promoter
    pTRE-tight (TetOn)
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    171004-plasmid-map-sequence-id-335832

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  29. pSMT3-FBF2 SS/Y

    Plasmid
    #166973
    Purpose
    Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Traci Hall
    Article
    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1
    Insert
    FBF-2 (fbf-2 Nematode)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166973-plasmid-map-sequence-id-331634

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  30. pSMT3-FBF2 AS/Y

    Plasmid
    #166974
    Purpose
    Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Traci Hall
    Article
    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1
    Insert
    FBF-2 (fbf-2 Nematode)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166974-plasmid-map-sequence-id-331632

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  35. p1A Prk-

    Plasmid
    #162694
    Purpose
    p1A negative control expressing CbbLS and inactive Prk (Prk K20M S21A)
    Depositor
    David Savage
    Article
    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1
    Insert
    H. neapolitanus CbbLS and S. elongatus Prk
    Use
    Tags
    N terminal 6x His tag on PRK
    Expression
    Bacterial
    Mutation
    Prk inactive (K20M S21A native protein, this map …
    Promoter
    PLtet0-1 promoter
    Available since
    Feb. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    162694-plasmid-map-sequence-id-324149

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  36. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

    Plasmid
    #123340
    Purpose
    Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-EKAR-EV(T/A)
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Target Thr residue in substrate domain mutated to…
    Promoter
    CMV
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    123340-plasmid-map-sequence-id-307135

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  38. pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

    Plasmid
    #123336
    Purpose
    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Cerulean3-cpCerulean3-FLARE-AKAR
    Use
    Tags
    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123336-plasmid-map-sequence-id-307044

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Showing: 2921 - 2960 of 7473 results