We narrowed to 23,118 results for: Sis
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Plasmid#139284PurposeCys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.DepositorInsertcfmTurquoise
TagsMulti-cloning sitesExpressionMammalianMutationCys48Ser and Cys70/MetAvailable SinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT/Caggs-NRAS G12V-IRES-ASK1
Plasmid#221074PurposeExpresses NRAS G12V and ASK1 in mammalian cellsDepositorAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10:AtFLS2
Plasmid#202186PurposeExpress Arabidopsis thaliana FLS2 gene under UBQ10 promoterDepositorAvailable SinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGMEH-T38M-750-luxAB-DAS+4
Plasmid#49520PurposeHygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomallyDepositorInsertT38M-P750-luxAB-DAS+4
TagsDAS+4 (AANDENYSENYADAS)ExpressionBacterialAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
SEP-Nanoluc-cyOFP1
Plasmid#141082Purposeprototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEP-Nanoluc-cyOFP1
UseLentiviralMutationDeletion of amino acids DISGG from positions 673 …PromoterEF1αAvailable SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCold I-Hero7
Plasmid#187926PurposeBacterial expression of heat-resistant obscure (Hero) protein Hero7DepositorAvailable SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Susd4(3.7)-EGFP-sv40
Plasmid#184413PurposeAAV expression of tTA from mouse sushi domain containing 4 (Susd4) gene promoter.DepositorInsertMouse Susd4 gene promoter-EGFP (Susd4 Mouse)
UseAAVTagsEGFPExpressionMammalianPromoterMouse Susd4 geneAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron(Neo)-G(LNL)
Plasmid#36908DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo
ExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEM-PH-citrine
Plasmid#131406PurposePurpose: synthesis of mRNA encoding a membrane marker. Insert: PH domain of the human phospholipase C delta 1 (PLCD1) fused with the yellow fluorescent protein mCitrineDepositorInsertPH
UseIn vitro transcriptionTagscitrine yellow fluorescent proteinPromoterT7Available SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
AID-BFP-loxP_myo2_neoR
Plasmid#194054PurposeDual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genomeDepositorAvailable SinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS
Plasmid#127365PurposeBinary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plantsDepositorInsertBirA* (R118G mutant)
TagsGS linker, NLS, V5, and YFPExpressionPlantMutationR118GPromoterArabidopsis UBQ10 promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZJQIBEBT003
Plasmid#140053PurposeThe pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.DepositorInserteMutS-L157C-G233C-CBM-EGFP
TagsHis-TagExpressionBacterialMutationL157C, G233CPromoterT7Available SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
sox10:PDGFRA wild-type
Plasmid#98696PurposeI-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafishDepositorInsertPDGFRA (PDGFRA Human)
UseZebrafish expressionAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
p35S::IRE1a
Plasmid#135232Purposeconstitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)DepositorAvailable SinceJan. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP
Plasmid#127363PurposeBinary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plantsDepositorInsertBirA* (R118G mutant)
TagsGS linker, NES, V5, and YFPExpressionPlantMutationR118GPromoterArabidopsis UBQ10 promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
sox10:PDGFRA mutant
Plasmid#98697PurposeI-SceI meganuclease-mediated human PDGFRA mutant transgenesis in zebrafishDepositorAvailable SinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_HindIII
Plasmid#65662PurposeFLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationintroduced HindIII site (final goal to exchange F…PromoterCMV, 2x TetAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron(Neo)-T(FLLFLNL)
Plasmid#40020DepositorInsertsGFP
tdT-3Myc
beta-globin intron
Neo
Tags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta globin promoter and CMV enhance…Available SinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE-FT_INF
Plasmid#112874Purposebinary vector for INF (Induced Neo-Functionalization) of FT in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA targeting FT
UseCRISPRExpressionPlantAvailable SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-SLC35B2-FLAG
Plasmid#154863PurposeTransient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2DepositorInsertSLC35B2 (SLC35B2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationctcctggtgcagtacttc => ttattagtccaatattttPromoterCMVAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBT250_(pCA-G-intron(Neo)-tTA2)
Plasmid#36876DepositorInsertsGFP
tTA2
beta-globin intron
Neo
ExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7
Plasmid#124859PurposeMutagenesis of Slc17a7DepositorInsertSlc17a7 (Slc17a7 Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Coh-ddFLN4-ELP-HIS-ybbr
Plasmid#153446PurposeE. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc.CohE
Tags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron(Neo)-T(LNL)
Plasmid#36907DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Neo
Tags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID(1-114)
Plasmid#99513PurposeTruncated C-terminal AID degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(1-114) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteMutationinternal inversion of nucleotides 265-312 of the …Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWZL-rCHES1
Plasmid#51847Purposeencodes a CHES1 mRNA that is resistant to shRNA against CHES1DepositorInsertCHES1 (FOXN3 Human)
UseRetroviralAvailable SinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CR-QUAS:NTR-mKate2
Plasmid#241914PurposeZebrafish transgenesis plasmid encoding nitroreductase-mKate2, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis markerDepositorInsertQUAS enhancer driving nitroreductase-mKate2
UseZebrafish tol2 transgenesisTagsnitroreductase fused with mKate2PromoterQUASAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUBC(k)-AT5G42080-mKOk
Plasmid#224852PurposePlasmid for expression of AT5G40280.1 coding sequence tagged with mKOk in plantsDepositorInsertAT5G40280.1 (ERA1 Mustard Weed)
ExpressionPlantAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUBC(k)-AT4G33650-mKOk
Plasmid#224846PurposePlasmid for expression of AT4G33650.1 coding sequence tagged with mKOk in plantsDepositorInsertAT4G33650.1 (DRP3A Mustard Weed)
ExpressionPlantAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
UBQ10pro-nlsABACUS2-400n-NosT
Plasmid#203728PurposeBinary vector for the transformation of the nlsABACUS2-400n biosensor for (+)-Abscisic acid into ArabidopsisDepositorInsertnlsABACUS2-400n
UseSynthetic BiologyTagsMycExpressionPlantPromoterAtUBQ10pro 1500bpAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a
Plasmid#213401PurposePlant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursorDepositorInsertArabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
ExpressionPlantPromoter2x35SAvailable SinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor
Plasmid#211905PurposeVector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.DepositorInsertDARIC-CD19-scFv
UseAAVExpressionMammalianPromoterhPGK1Available SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 genomic A. thaliana PLDGAMMA3
Plasmid#209188PurposepENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tagsDepositorAvailable SinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
1BR9-AtWUSSTOP
Plasmid#202053PurposeE. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHELDepositorInsertAtWUS E. Coli Codon Optimized (WUS Mustard Weed)
Tags6xHis and GFP11ExpressionBacterialPromoterT7 PromoterAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-MD-Gata2
Plasmid#193090Purposeconstitutive expression of the mitosis degradation (MD) domain of cyclin B1 fused to mouse Gata2 in mammalian cellsDepositorInsertMD-Gata2
UseLentiviralExpressionMammalianPromoterUbcAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUW-MDmut-mTurquoise
Plasmid#193095Purposeconstitutive expression of a mutated (mut, R42A), non-functional version of the mitosis degradation (MD) domain of cyclin B1 fused to mTurquoise in mammalian cellsDepositorInsertMDmut-mTurquoise
UseLentiviralExpressionMammalianPromoterUbcAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CALPAIN-7-mCherry, F98D
Plasmid#180653PurposeLentiviral expression vector for CALPAIN-7. Used for generating cell lines. F98D mutation. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-53.DepositorInsertCALPAIN-7
UseLentiviralTagsmCherryExpressionMammalianMutationsiRNA resistant to: GCACCCAUACCUUUACAUUAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMA5460
Plasmid#184855PurposeEncodes G418 resistance and PhiC31-excisable hTFAM geneDepositorInsertTFAM (TFAM Human)
UseRetroviral; Phic31 attp-attb recombination/excisi…ExpressionMammalianMutationWTPromoterLTRAvailable SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA4000
Plasmid#184857PurposeCre-excisable TFAM in Blasticidin-resistannce retroviral vectorDepositorAvailable SinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CDS12_Cas9-NLS
Plasmid#136122PurposePuchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAGDepositorInsertCDS12_AtcoCas9-NLS(noStop)
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC_N168C His
Plasmid#173809PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
Tags6XHisExpressionBacterialMutationAsn168Cys for labelingPromoterT7Available SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
HBT-CPK32ac-Flag
Plasmid#107431PurposeFor Arabidopsis mesophyll protoplast transient expression assayDepositorAvailable SinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc C1A4E
Plasmid#141088Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of NanolucDepositorInsertAntares-T2A-puromycin-IRESv24-SEP-C1A4E
ExpressionMammalianMutationPlease see depositor commentsPromoterCMVAvailable SinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-161K enhancer-NR2F2-MUT
Plasmid#138371PurposeGateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutatedDepositorInsert161k human enhancer with conserved NR2F2 binding sites mutated
UseGateway compatibleMutationNR2F2 sites mutatedPromoternoneAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-161K enhancer-ETS-CORE-MUT
Plasmid#138372PurposeGateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutatedDepositorInserthuman 161K human enhancer with ETS bind sites mutated
UseGateway compatibleMutationETS factor binding sites mutatedPromoterNONEAvailable SinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
hHSF4b K294R
Plasmid#118357PurposeThis plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.DepositorAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gal4-HSF4b WT
Plasmid#118358PurposeThis plasmid expresses amino-acids 200-493 of hHSF4b WT fused to the GAL-4 DNA binding domain.DepositorAvailable SinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHyg-AID(31-114)
Plasmid#99514PurposeTruncated C-terminal AID degron cassette with HygR marker for S. cerevisiaeDepositorInsertIAA17(31-114) (AXR3 Mustard Weed)
UsePcr template for yeast tagging cassetteMutationinternal inversion of nucleotides 265-312 of the …Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1474
Plasmid#82420PurposePlasmid for expression of HA-tagged N-terminal region of Nematostella vectensis GW182 (aa 1-1158) in mammalian cellsDepositorInsertnvGW182 NED
TagsHAExpressionMammalianMutationdeleted amino acids 1159-1698 from nvGW182PromoterCMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCh-1475
Plasmid#82421PurposePlasmid for expression of HA-tagged C-terminal region of Nematostella vectensis GW182 (aa 1159-1698) in mammalian cellsDepositorInsertnvGW182 CED
TagsHAExpressionMammalianMutationdeleted amino acids 1-1158 from nvGW182PromoterCMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only