We narrowed to 9,223 results for: Coli

1. pNK141b2

   Plasmid

   #156375

   Purpose

   Expresses orthogonal RBS mTagBFP reporter

   Depositor

   Ahmed Badran

   Article

   Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

   Insert

   mTagBFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1 FLIPglu-600uDelta13V

   Plasmid

   #18014

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   FLIPglu F16A Delta13
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   F16A in binding protein

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTK164

   Plasmid

   #13533

   Depositor

   Wolf Frommer

   Article

   Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

   Insert

   FLIPW-CTY
   

   Use

   Tags

   ECFP and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNIC-NHSTIIT-AVITAG-GG

   Plasmid

   #215802

   Purpose

   Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pRM2

   Plasmid

   #8486

   Depositor

   Nancy Craig

   Article

   McKown et al J Bacteriol 1988 Jan;170(1):352-8.

   Insert

   attTn7 (tnsA Bacteria)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pp2.1-PT5-lacI-gusA-specR-pp2.2-IMBB

   Plasmid

   #30505

   Depositor

   Ichiro Matsumura

   Article

   Murin et al Appl Environ Microbiol. 2011 Oct 21.

   Insert

   beta-glucuronidase (gusA Escherichia coli)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 FLIPglu-30uDelta13V

   Plasmid

   #18015

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   FLIPglu D154A Delta13
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   mglB-D154A

   Promoter

   Available since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pXDC61

   Plasmid

   #21841

   Depositor

   Howard Shuman

   Article

   Charpentier et al PLoS Pathog. 2009 Jul . 5(7):e1000501.

   Insert

   blaM
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKP325

   Plasmid

   #159596

   Purpose

   Express E.coli TonB protein

   Depositor

   Kathleen Postle

   Article

   Larsen et al Mol Microbiol. 1999 Mar;31(6):1809-24. d

   Insert

   tonB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAPA3

    Plasmid

    #24777

    Depositor

    Tanya Parish

    Article

    Parish et al J Bacteriol. 2007 May . 189(10):3721-8.

    Type

    Empty backbone

    Use

    Integrating vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNIC-TolB-NHSTIIT-GG

    Plasmid

    #215792

    Purpose

    Cloning of target gene with N terminal TolB SRP pathway signal peptide N terminal His6 Twin Strep tag and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pRS303-LacZ

    Plasmid

    #1169

    Depositor

    Susan Lindquist

    Article

    Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

    Insert

    LACZ
    

    Use

    Tags

    GAL1:10 promoter

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pSelect-6

    Plasmid

    #190243

    Purpose

    Encodes lacI- and GFP-targeting gRNAs for positive and negative selections of dCas9 activity.

    Depositor

    Marc Ostermeier

    Article

    Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

    Insert

    LacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC23-LIC-His-FLAG

    Plasmid

    #165477

    Purpose

    pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    TEV-FLAG-10x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKSJ155

    Plasmid

    #27126

    Depositor

    Alan Finkelstein

    Article

    Kienker et al J Gen Physiol. 2000 Oct . 116(4):587-98.

    Insert

    Colicin Ia + Ia immunity protein
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1 FLIPglu-3.2mDelta13V

    Plasmid

    #18011

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu F16A S112A Delta13
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    F16A, S112A in binding protein

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSET FLIPmal-2u

    Plasmid

    #14479

    Depositor

    Wolf Frommer

    Article

    Fehr et al Proc Natl Acad Sci U S A. 2002 Jul 23. 9

    Insert

    FLIPmal
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    5AA

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNIC-PelB-NH10T-AVITAG-GG

    Plasmid

    #215793

    Purpose

    Cloning of target gene with N terminal PelB SEC pathway signal peptide His10 and AVITAG and TEV cleavage

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pNIC-HIS6-GST-TEV-AVITAG-GG

    Plasmid

    #215809

    Purpose

    Cloning of target gene with N terminal His6 GST tag and TEV cleavage non cleavable N-terminal avitag

    Depositor

    ASAP Discovery, Frank von Delft

    Article

    Fairhead et al bioRxiv 2024.02.13.579886

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCYP03

    Plasmid

    #212836

    Purpose

    Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Insert

    cytochrome P450
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PcymRC

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFF145

    Plasmid

    #61446

    Purpose

    aTc-inducible Beta recombinase

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    Beta recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pTetO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSF-DUET Cas1 Cas2

    Plasmid

    #80088

    Purpose

    Encodes Cas1 and Cas2

    Depositor

    George Church

    Article

    Shipman et al Science. 2016 Jun 9. pii: aaf1175.

    Inserts

    Cas1 (cas1 E. coli (K12))
    Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDBD-M

    Plasmid

    #50572

    Purpose

    Double Ub M-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50571

    Depositor

    Alexander Varshavsky

    Article

    Turner et al Science. 2000 Sep 22;289(5487):2117-20.

    Insert

    DHFR-Ubiquitin-MetBetaGal-Ubiquitin-DHFR-Ubiquitin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pDBD-R

    Plasmid

    #50571

    Purpose

    Double Ub R-ek Beta-gal construct for ubiquitin sandwich assay, for measurement of cotranslational protein degradation, use with 50572

    Depositor

    Alexander Varshavsky

    Article

    Turner et al Science. 2000 Sep 22;289(5487):2117-20.

    Insert

    DHFR-Ubiquitin-ArgBetaGal-Ubiquitin-DHFR-Ubiquitin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    M1R (B-gal)

    Promoter

    Gal1

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pCRISPR

    Plasmid

    #113252

    Purpose

    pUC19 backbone with E. coli CRISPR array

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    E. coli CRISPR array
    

    Use

    Integration substrate

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET29b-ClbP

    Plasmid

    #48244

    Purpose

    Untagged, full-length protein for in vivo peptidase assays

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbP
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFF714

    Plasmid

    #61449

    Purpose

    IPTG-inducible SCRIBE_galK(OFF)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    SCRIBE_galK(OFF)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO

    Available since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 FLIPglu-170nDelta13V

    Plasmid

    #18018

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu F16F Delta13
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    WT binding protein (no F16A)

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUS200

    Plasmid

    #17788

    Depositor

    Stephen Sligar

    Article

    Unger et al J Biol Chem. 1986 Jan 25. 261(3):1158-63

    Insert

    Cytochrome P450cam
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pClodANL-Cherry

    Plasmid

    #107870

    Purpose

    Bacterial expression of cherry-NanoLuc

    Depositor

    Sanford Simon

    Article

    Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

    Insert

    Cherry (red fluorescent protein)
    

    Use

    Luciferase

    Tags

    NanoLuc

    Expression

    Bacterial

    Mutation

    Promoter

    pro D

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge