166,806 results

1. pKK-BiFC-Venus

   Plasmid

   #105804

   Purpose

   Concomitant expression of two proteins in fusion with fragments of Venus protein. Protein interactions studies by bimolecular fluorescence complementation (BiFC), including BiFC-FRET assays.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   VN173 (I152L); VC155

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPRv2_mSTING_gRNA_2

   Plasmid

   #196626

   Purpose

   Knock-out STING in murine cells, gRNA 2.

   Depositor

   Nicolas Manel

   Article

   Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

   Insert

   mSTING gRNA 2 (Sting1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC1-HyPerRed-mito

   Plasmid

   #60247

   Purpose

   Mitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

   Insert

   HyPerRed-mito
   

   Tags

   Mitochondrial targeting signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAF0687 Pa01_attP-mCherry donor

   Plasmid

   #193466

   Purpose

   Promoterless mcherry donor for Pa01

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01_attP
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-7 asyn A53T

   Plasmid

   #105727

   Purpose

   Bacterial expression of mutant human alpha synuclein

   Depositor

   Hilal Lashuel

   Article

   Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

   Insert

   alpha-synuclein A53T (SNCA Human)
   

   Expression

   Bacterial

   Mutation

   A53T

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-Rab7A Q67L

   Plasmid

   #28049

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

   Insert

   Rab7A (RAB7A Human)
   

   Tags

   2xHA and EGFP

   Expression

   Mammalian

   Mutation

   Q67L

   Available Since

   May 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCherry- SEC61B

   Plasmid

   #121160

   Purpose

   Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   SEC61B (SEC61B )
   

   Expression

   Mammalian

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLJM60 FLAG-RAB35 Q67L

   Plasmid

   #107105

   Purpose

   Lentiviral vector that codes for FLAG-RAB35 Q67L (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.

   Depositor

   Charles Sawyers

   Article

   Wheeler et al Science. 2015 Oct 9;350(6257):211-7. doi

   Insert

   RAB35 (RAB35 Human)
   

   Use

   Lentiviral

   Tags

   FLAG tag

   Expression

   Mammalian

   Mutation

   Q67L

   Promoter

   CMV

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CAG-jGCaMP8s-WPRE

   Plasmid

   #179256

   Purpose

   AAV-mediated expression of GCaMP8s under the CAG promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8s
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29a-human histone 4-in-One

    Plasmid

    #223237

    Purpose

    Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

    Depositor

    Weixing Zhao

    Inserts

    His6-VSVG-Histone H2A
    Histone H2B
    Histone H3
    Histone H4-TEV-His6-Dual StrepII
    

    Tags

    Dual StrepII, His6, TEV protease site, and VSVG

    Expression

    Bacterial

    Mutation

    A103G

    Promoter

    T7

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. FU-H2B-GFP-IRES-Puro

    Plasmid

    #69550

    Purpose

    Lentiviral, selectable fluorescent GFP tagging of nuclei.

    Depositor

    Charles Gersbach

    Insert

    Histone 2B - green fluorescent protein fusion
    

    Use

    Lentiviral

    Tags

    GFP fusion

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. 2C::tdTomato Reporter

    Plasmid

    #40281

    Depositor

    Samuel Pfaff

    Article

    Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

    Insert

    2C Promoter (2-cell stage promoter)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Available Since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXsIP GFP-VMP1

    Plasmid

    #117417

    Purpose

    Expresses VMP1 tagged with GFP in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

    Insert

    VMP1 (VMP1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti6.3 GCN1-3xFlag_IRES-iRFP

    Plasmid

    #198388

    Purpose

    GCN1 expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    GCN1 (GCN1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Klotho (secreted)

    Plasmid

    #17713

    Depositor

    Hal Dietz

    Article

    Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

    Insert

    Klotho (KL Human)
    

    Tags

    His and V5

    Expression

    Mammalian

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-mCherry-G12V-KRAS-IRES-Blast

    Plasmid

    #153336

    Purpose

    Lentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cells

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    mCherry-G12V-KRAS (KRAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-Halotag-FRB-EX-nes_pLX304

    Plasmid

    #120913

    Purpose

    Lentiviral vector expressing HA-Halotag-FRB-EX-NES in mammalian cytosol

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    HA-Halotag-FRB-EX-NES
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-AID-PcrA M6-UGI-T2A-mTagBFP2

    Plasmid

    #221225

    Purpose

    Express HACE Editor as a fusion of AID cytidine deaminase and an optimized PcrA M6 helicase with mTagBFP2 reporter

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    AID-PcrA M6-UGI-T2A-mTagBFP2
    

    Expression

    Mammalian

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pfwB

    Plasmid

    #37329

    Depositor

    John Schiller

    Article

    Buck et al J Virol. 2005 Mar;79(5):2839-46.

    Insert

    GFP
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMRX-IB-pSu9-HaloTag7-mGFP

    Plasmid

    #184905

    Purpose

    Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    Presequence of Fo-ATPase subunit 9
    

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Available Since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pEGFP-N1-Tspan4

    Plasmid

    #210323

    Purpose

    Expresses Tetraspanin-4 (TSPAN4) as migrasome marker

    Depositor

    Li Yu

    Article

    Ma et al Cell Res. 2015 Jan;25(1):24-38. doi: 10.

    Insert

    Tetraspanin 4 (Tspan4 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV3flag8HOIP

    Plasmid

    #50015

    Purpose

    Mammalian expression of human HOIP (RNF31) with a c-terminal 3xFLAG tag, and hygromycin selection.

    Depositor

    Martin Dorf

    Article

    Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

    Insert

    ring finger protein 31 (RNF31 Human)
    

    Tags

    3 x FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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23. PX458-mcherry

    Plasmid

    #161974

    Purpose

    Expresses Cas9-p2a-mcherry (BbsI site silenced) and sgRNA

    Depositor

    Joanna Wysocka

    Article

    Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

    Insert

    Cas9-t2a-mcherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHM6-Q74

    Plasmid

    #40264

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Tags

    6x His and HA

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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25. ptf-Galectin3

    Plasmid

    #64149

    Purpose

    Expression in mammalian cells

    Depositor

    Tamotsu Yoshimori

    Article

    Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

    Insert

    Galectin3 (LGALS3 Human)
    

    Tags

    EGFP and mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV.hSynapsin.SF-iGluSnFR.A184S

    Plasmid

    #106174

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1 and AAV9

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S

    Promoter

    hSynapsin

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-Fos-CreERT2

    Plasmid

    #194643

    Purpose

    Expresses Cre-ERT2 under the control of c-Fos promoter

    Depositor

    Jun-Hyeong Cho

    Article

    Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

    Insert

    Fos-CreERT2
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Jan. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. pHM6-Q23

    Plasmid

    #40263

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q23 (HTT Human)
    

    Tags

    6x His and HA

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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29. pGEX6P1-mCherry-Nanobody (LaM-2)

    Plasmid

    #162276

    Purpose

    Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    mCherry-Nanobody (LaM-2)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAAV MP-84 mRFP

    Plasmid

    #206926

    Purpose

    The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.

    Depositor

    Markus Grompe

    Article

    Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

    Insert

    mRFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    MP-84

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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