We narrowed to 2,315 results for: crispr system

1. pCMV-SaPE2

   Plasmid

   #174817

   Purpose

   Mammalian expression of SaCas9 prime editor 2

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   SaPE2
   

   Use

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   Detailed in manuscript

   Promoter

   CMV

   Available since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSH-EFIRES-B-AtAFB2-mCherry

   Plasmid

   #129718

   Purpose

   AAVS1 safe harbor targeting vector with blasticidin resistance gene expressing AtAFB2-mCherry

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF1a

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLP339 pGK-dCas9-SunTag

   Plasmid

   #100943

   Purpose

   dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, SunTag array (10xGCN4)
   

   Use

   CRISPR

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLP457 pGK-dCas9-Suntag-BFP

   Plasmid

   #100957

   Purpose

   BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, SunTag array (10xGCN4)
   

   Use

   CRISPR

   Tags

   3xHA and BFP

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSH-EFIRES-P-Seipin-miniIAA7-3XFlag

   Plasmid

   #129722

   Purpose

   AAVS1 safe harbor targeting vector expressing miniIAA7-3XFlag tagged Seipin

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   Seipin (BSCL2 Human)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   miniIAA7-3XFlag

   Expression

   Mammalian

   Mutation

   silent mutations

   Promoter

   EF1a

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR_PGK_antiCD19_eZ3_Gal4VP64

   Plasmid

   #169917

   Purpose

   Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

   Depositor

   Zhili Rong

   Article

   Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

   Insert

   antiCD19-Notch3-GL4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Additional EGF repeat between the extracellular d…

   Promoter

   PGK

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-hSyn-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

   Plasmid

   #177345

   Purpose

   AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from Synapsin promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   dead hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3x GCN4 peptide, 3xHA, and NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   Synapsin

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHS1859

   Plasmid

   #205972

   Purpose

   FLAG-SV40 NLS-Csy3/Cas7 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Csy3/Cas7 from JAGBLH010000220, Cas8-HNH
   

   Use

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHS1860

   Plasmid

   #205973

   Purpose

   FLAG-SV40 NLS-Cas6/Csy4 from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas6/Csy4 from JAGBLH010000220, Cas8-HNH
   

   Use

   Tags

   FLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. human ETV6 gRNA-1

    Plasmid

    #133353

    Purpose

    human ETV6 gRNA-1 is a gRNA expression plasmid. Its 20-nt specific sequence targets the first ETV6 exon.

    Depositor

    Linzhao Cheng

    Article

    Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

    Insert

    ETV6 (ETV6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSH-EFIRES-P-Seipin-miniIAA7-mCherry

    Plasmid

    #129720

    Purpose

    AAVS1 safe harbor targeting vector expressing miniIAA7-mCherry tagged Seipin

    Depositor

    Elina Ikonen

    Article

    Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

    Insert

    Seipin (BSCL2 Human)
    

    Use

    CRISPR and TALEN; Human safe harbor locus (a avs1…

    Tags

    miniIAA7-mCherry

    Expression

    Mammalian

    Mutation

    silent mutations

    Promoter

    EF1a

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

    Plasmid

    #87906

    Purpose

    sgRNA targeting GFP

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgGFP1, sgGFP2, sgGFP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1, U6, 7SK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNS19-LV-mutRFP-2A-GFP

    Plasmid

    #113716

    Purpose

    Lentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    mutRFP-2A-GFP-IRES-Puro
    

    Use

    Lentiviral; Reporter system

    Tags

    Expression

    Mammalian

    Mutation

    Y64L (mutRFP)

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA

    Plasmid

    #176461

    Purpose

    Vector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMV

    Depositor

    Hui Yang

    Article

    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

    Insert

    BbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. gfap-EGFP donor

    Plasmid

    #65564

    Purpose

    Intron targeting-mediated EGFP knockin at the zebrafish gfap locus

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    gfap-EGFP (gfap Zebrafish)
    

    Use

    Zebrafish injection

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKI-cjFOXP2-hum

    Plasmid

    #129757

    Purpose

    Gene targeting in marmoset cells

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

    Insert

    FOXP2 (LOC100411307 C. jacchus (common marmost))
    

    Use

    Marmoset targeting

    Tags

    Expression

    Mutation

    T301N/N323S

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMuLE ENTR U6 stuffer sgRNA scaffold R4-R3

    Plasmid

    #62131

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Tol2-LyzC-Rac-WT(guide resistant)-2a-mcherry-U6ac-Rac guides

    Plasmid

    #168242

    Purpose

    "Rescue Rac expression in neutrophil specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Rac(guide resistant)-WT
    

    Use

    Zebrafish expression

    Tags

    mcherry

    Expression

    Mutation

    Promoter

    LyzC

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMuLE ENTR U6 stuffer sgRNA scaffold L1-L4

    Plasmid

    #62128

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMuLE ENTR U6 stuffer sgRNA scaffold L1-R5

    Plasmid

    #62127

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and sgRNA scaffold module for gRNA expression. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Type

    Empty backbone

    Use

    CRISPR; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge