We narrowed to 5,818 results for: pCas

1. pX330_ACTB-i2 sgRNA / hSpCas9

   Plasmid

   #172826

   Purpose

   Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAFNF-Venus-Actb

   Plasmid

   #169788

   Purpose

   An actin FRET sensor. Must be expressed with ECFP-actin for FRET.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Actb (Actb Mouse)
   

   Tags

   Venus

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-ChroMD-mRuby2-ST

   Plasmid

   #171158

   Purpose

   Cation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroMD
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-EGxxFP-PIGA

   Plasmid

   #153958

   Purpose

   Used for EGxxFP assay with PIGA intron 5 and exon 6 sequences

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   PIGA (a 401-bp fragment from intron 5 and exon 6)
   

   Use

   A plasmid specific for egxxfp assay

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CAG promoter

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-EGxxFP-CD55

   Plasmid

   #153959

   Purpose

   Used for EGxxFP assay with CD55 intron 3, exon 4, and intron 4 sequences

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   CD55 (a 1,016-bp fragment from intron 3, exon 4, and intron 4)
   

   Use

   A plasmid specific for egxxfp assay

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   No mutation

   Promoter

   CAG promoter

   Available Since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-BRCA1-RNF169UBD

   Plasmid

   #119009

   Purpose

   Mammalian expression of a fusion protein of human BRCA1 with the human RNF169 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   BRCA1 (BRCA1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Gal4-RNF169UBD

   Plasmid

   #119013

   Purpose

   Mammalian expression of fusion protein of yeast Gal4 with human RNF169 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   Gal4 (GAL4 Budding Yeast)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-BRCA1-Rad18UBD

   Plasmid

   #119008

   Purpose

   Mammalian expression of a fusion protein of human BRCA1 with human Rad18 Ubiquitin binding domain and BFP expression

   Depositor

   Ralf Kuehn

   Article

   Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

   Insert

   BRCA1 (BRCA1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCASP-SptP35-TD-HilA

   Plasmid

   #153332

   Purpose

   Triggers expression of SptP35 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

   Depositor

   Josef Penninger

   Article

   Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

   Insert

   HA4-7c12 Tandem Monobody
   

   Use

   Salmonella expression

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCASP-SptP92-TD-HilA

    Plasmid

    #153331

    Purpose

    Triggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    SptP92-HA4-7c12 Tandem Monobody
    

    Use

    Salmonella expression

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCASP-3G124-HilA

    Plasmid

    #153324

    Purpose

    Shows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vector

    Depositor

    Josef Penninger

    Article

    Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

    Insert

    3G124 DARPin but no expression
    

    Use

    Salmonella expression

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. eSpCas9(1.1)-Cdt1

    Plasmid

    #122342

    Purpose

    Expresses sgRNA targeting mouse Cdt1 and eSpCas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Cdt1
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-FLAG-B-eSpCas9

    Plasmid

    #126772

    Purpose

    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A; K1003A; R1060A; amino acids 1005-1013 repl…

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. BII-ChPtW-eSpCas9

    Plasmid

    #133381

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-Ace1_Q89D_D100N_E206V-HaloTag

    Plasmid

    #129255

    Purpose

    Express Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace1_Q89D_D100N_E206V-HaloTag
    

    Expression

    Mammalian

    Mutation

    Q89D, D100N, E206V

    Promoter

    CAG

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-Mac_Q139D_D150N_E258V-HaloTag-ST

    Plasmid

    #129264

    Purpose

    Express soma-localized Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Mac_Q139D_D150N_E258V-HaloTag-ST
    

    Expression

    Mammalian

    Mutation

    Q139D,D150N,E258V

    Promoter

    CAG

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag-ST

    Plasmid

    #129263

    Purpose

    Express soma-localized QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    QuaAr3_Q95D_H106N_E214V-HaloTag-ST
    

    Expression

    Mammalian

    Mutation

    Q95D, H106N, E214V

    Promoter

    CAG

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST

    Plasmid

    #129260

    Purpose

    Express soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Ace1_Q89D_D100N_E206V-HaloTag-ST
    

    Expression

    Mammalian

    Mutation

    Q89D, D100N, E206V

    Promoter

    CAG

    Available Since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Mac_Q139D_D150N_E258V-HaloTag

    Plasmid

    #129259

    Purpose

    Express Mac_Q139D_D150N_E258V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Mac_Q139D_D150N_E258V-HaloTag
    

    Expression

    Mammalian

    Mutation

    Q139D,D150N,E258V

    Promoter

    CAG

    Available Since

    Aug. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-QuaAr3_Q95D_H106N_E214V-HaloTag

    Plasmid

    #129258

    Purpose

    Express QuaAr3_Q95D_H106N_E214V-HaloTag in mammalian cells

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    QuaAr3_Q95D_H106N_E214V-HaloTag
    

    Expression

    Mammalian

    Mutation

    Q95D, H106N, E214V

    Promoter

    CAG

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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