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SPLINTR GFP v1 backbone

Plasmid

#179777

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN-C

Plasmid

#30224

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 May;8(5):424-9. doi: 1

Insert

rhVitronectin with C terminus cleavage (VTN Human)

Expression

Bacterial

Mutation

deleted amino acids: 399-478

Available Since

May 24, 2011

Availability

Academic Institutions and Nonprofits only

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pSALECT-Efnb2

Plasmid

#59707

Purpose

pSALECT with Efnb2 insert

Depositor

Christopher Anderson

Article

Hsiau et al PLoS One. 2015 Mar 19;10(3):e0119927. do

Insert

Efnb2 (Efnb2 Mouse)

Expression

Bacterial

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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UCK2_Deletion_gRNA_2

Plasmid

#204673

Purpose

Dual gRNA plasmid for UCK2 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

uridine-cytidine kinase 2 (UCK2 Human)

Use

Lentiviral

Promoter

U6

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-SVA-lncRNA-AK057321-mcherry

Plasmid

#202813

Purpose

Lentiviral vector to express of SVA-lncRNA AK057321 with CMV for mcherry expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 1S scramble

Plasmid

#202837

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1S

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321 shRNA 1S scramble control

Use

Lentiviral

Expression

Mammalian

Promoter

6

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 2S scramble

Plasmid

#202839

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 2S

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

SVA-lncRNA AK057321 shRNA 2S scramble control

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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SVA-lncRNA AK057321 shRNA 1A scramble control

Plasmid

#202835

Purpose

Lentiviral vector that expresses a scrambled control shRNA for SVA-lncRNA AK057321 shRNA 1A

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

pLVX-SVA-lncRNA shRNA 1A scramble control

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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LRRK2-WT

Plasmid

#17609

Depositor

Ted Dawson

Article

West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

Insert

LRRK2 (LRRK2 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-A315T

Plasmid

#133755

Purpose

Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed alanine 315 to threonine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-M337V

Plasmid

#133759

Purpose

Lentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed methionine 337 to valine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-Q331K

Plasmid

#133761

Purpose

Lentiviral expression of human TDP-43 Q331K disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed glutamine 331 to lysine.

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-Bsd-CMV-hUCK

Plasmid

#195141

Purpose

UCK2 overexpression vector with blasticidin resistance

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

UCK2 (UCK2 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1_gRNA

Plasmid

#196139

Purpose

gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

AAVS1 gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-K263E

Plasmid

#133758

Purpose

Lentiviral expression of human TDP-43 K263E disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed lysine 263 to glutamate.

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Chr1q_Centromere-Targeting_gRNA

Plasmid

#195125

Purpose

gRNA targeting centromere-proximal location on Chromosome 1q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-luciferase-reporter-JAM2-SVA

Plasmid

#202832

Purpose

pGL3 luciferase reporter with JAM2 SVA cloned upstream of SV40 promoter to test effects of SVA insertion on luciferase expression

Depositor

Matthew Anderson

Article

Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

Insert

JAM2 intronic SVA

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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Chr7_Centromere-Targeting_gRNA

Plasmid

#195129

Purpose

dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr7 gRNA

Expression

Mammalian

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.1

Plasmid

#91895

Purpose

sgNf1.1 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.1

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.4

Plasmid

#92029

Purpose

sgNf1.4 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.4 for Nf1 deletion

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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