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We narrowed to 5,238 results for: codon optimized

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Showing: 3801 - 3850 of 5238 results

  1. B-HeFSpCas9-A926Q

    Plasmid
    #207370
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A926Q
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207370-plasmid-map-sequence-id-410164

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  2. B-evoSpCas9-E526K

    Plasmid
    #207365
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-E526K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    M495V, Y515N, R661Q, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207365-plasmid-map-sequence-id-410163

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  3. B-evoSpCas9-V495M

    Plasmid
    #207363
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-V495M
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Y515N, K526E, R661Q, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    207363-plasmid-map-sequence-id-410136

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  4. 1BR9-AtWUSSTOP-Δα3

    Plasmid
    #202056
    Purpose
    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL with the third helix in the homeodomain deleted.
    Depositor
    Markita Landry
    Article
    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:
    Insert
    AtWUS E. Coli Codon Optimized, third helix of homeodomain deleted (WUS Mustard Weed)
    Tags
    6xHis and GFP11
    Expression
    Bacterial
    Mutation
    third helix of homeodomain deleted (AA82-102)
    Promoter
    T7 Promoter
    Available Since
    Oct. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    202056-plasmid-map-sequence-id-409953

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  5. B-HeFSpCas9-A1060R

    Plasmid
    #207372
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A1060R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …
    Promoter
    CBh
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207372-plasmid-map-sequence-id-408921

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  6. B-HeFSpCas9-A1003K

    Plasmid
    #207371
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A1003K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …
    Promoter
    CBh
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    207371-plasmid-map-sequence-id-408916

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  7. B-HeFSpCas9-A848K

    Plasmid
    #207369
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A848K
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207369-plasmid-map-sequence-id-408749

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  8. B-HeFSpCas9-A661R

    Plasmid
    #207367
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-HeFSpCas9-A661R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…
    Promoter
    CBh
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207367-plasmid-map-sequence-id-408748

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  9. B-evoSpCas9-Q661R

    Plasmid
    #207366
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-Q661R
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207366-plasmid-map-sequence-id-408747

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  10. B-evoSpCas9-N515Y

    Plasmid
    #207364
    Purpose
    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    B-evoSpCas9-N515Y
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    M495V, K526E, R661Q, amino acids 1005-1013 replac…
    Promoter
    CBh
    Available Since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    207364-plasmid-map-sequence-id-408746

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  11. HypaR-SpCas9

    Plasmid
    #126757
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    Hypa2SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, N692A, M694A, Q695A, H698A
    Promoter
    CBh
    Available Since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    126757-plasmid-map-sequence-id-412372

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  12. pNMSB96

    Plasmid
    #199316
    Purpose
    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR at ttTi5605
    Depositor
    Michael Krieg
    Article
    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4
    Insert
    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR
    Expression
    Worm
    Mutation
    mTagBFP2 from pJJR81, C. elegans codon optimized …
    Promoter
    flp-18
    Available Since
    Aug. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    199316-plasmid-map-sequence-id-404765

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  14. GB1-ΔN-CHMP4C(S191C,I225A,L228A,W231A)

    Plasmid
    #199242
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C,I225A,L228A,W231A)
    Depositor
    Lalit Deshmukh
    Article
    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi
    Insert
    GB1-His6-TEV-CHMP4C(121-233, S191C, I225A, L228A, W231A) (CHMP4C Human)
    Tags
    GB1-His6-TEV
    Expression
    Bacterial
    Mutation
    S191C, I225A, L228A, W231A
    Available Since
    June 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    199242-plasmid-map-sequence-id-393310

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  16. Sniper2P

    Plasmid
    #193857
    Purpose
    Expresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Chem Biol. 2023 Mar 9. doi: 10.1038/
    Insert
    Sniper2L
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    E1007L
    Promoter
    EFS
    Available Since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    193857-plasmid-map-sequence-id-382456

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  17. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194889
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-1 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA1
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    194889-plasmid-map-sequence-id-382866

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  18. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194893
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and crRNA-2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available Since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    194893-plasmid-map-sequence-id-382802

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  38. GB1-PRD(703-868, P801G, T805C, C813S)-Strep

    Plasmid
    #169749
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, T805C, C813S)-Strep.
    Depositor
    Lalit Deshmukh
    Article
    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine, Threonine 805 to Cysteine…
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    169749-plasmid-map-sequence-id-333025

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  39. GB1-PRD(703-868, P801G, G808C, C813S)-Strep

    Plasmid
    #169750
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep.
    Depositor
    Lalit Deshmukh
    Article
    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G, G808C, C813S)-Strep (PDCD6IP Human)
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine, Glycine 808 to Cysteine, …
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    169750-plasmid-map-sequence-id-333026

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  41. GB1-PRD(703-868, S712C, P801G, C813S)-Strep

    Plasmid
    #164445
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep.
    Depositor
    Lalit Deshmukh
    Article
    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10
    Insert
    GB1-His6-TEV-ALIX(703-868, S712C, P801G, C813S)-Strep (PDCD6IP Human)
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Serine 712 to Cysteine, Proline 801 to Glycine, C…
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164445-plasmid-map-sequence-id-324032

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  42. GB1-PRD(703-868, A756C, P801G, C813S)-Strep

    Plasmid
    #164446
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep.
    Depositor
    Lalit Deshmukh
    Article
    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10
    Insert
    GB1-His6-TEV-ALIX(703-868, A756C, P801G, C813S)-Strep (PDCD6IP Human)
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Alanine 756 to Cysteine, Proline 801 to Glycine, …
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164446-plasmid-map-sequence-id-324033

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  43. GB1-PRD(703-868, P801G, C813S, S863C)-Strep

    Plasmid
    #164447
    Purpose
    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep.
    Depositor
    Lalit Deshmukh
    Article
    Elias et al J Biol Chem. 2021 Oct 21:101328. doi: 10
    Insert
    GB1-His6-TEV-ALIX(703-868, P801G, C813S, S863C)-Strep (PDCD6IP Human)
    Tags
    GB1-His6-TEV and Strep tag (WSHPQFEK)
    Expression
    Bacterial
    Mutation
    Proline 801 to Glycine, Cysteine 813 to Serine, S…
    Available Since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164447-plasmid-map-sequence-id-324034

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  44. vPyACR_2164382

    Plasmid
    #153029
    Purpose
    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).
    Depositor
    Peter Hegemann
    Article
    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.
    Insert
    vPyACR_2164382
    Tags
    eYFP
    Expression
    Mammalian
    Mutation
    vPyACR_2164382 was C-terminally truncated to incl…
    Promoter
    CMV (+ enhancer)
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    153029-plasmid-map-sequence-id-300511

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  45. vPyACR_21821

    Plasmid
    #153030
    Purpose
    Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).
    Depositor
    Peter Hegemann
    Article
    Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.
    Insert
    vPyACR_21821
    Tags
    eYFP
    Expression
    Mammalian
    Mutation
    vPyACR_21821 was C-terminally truncated to includ…
    Promoter
    CMV (+ enhancer)
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    153030-plasmid-map-sequence-id-300513

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  50. pCEP4-001428(753)-VC

    Plasmid
    #123256
    Purpose
    Mammalian expression plasmid for Env from the 001428 HIV-1 isolate; C-terminal truncation fused to the C-terminal half of split fluorescent Venus (VC)
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (001428) Env
    Tags
    CD5 leader peptide and VC (Venus residues D155–K2…
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene; C-terminal Env re…
    Promoter
    CMV
    Available Since
    April 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    123256-plasmid-map-sequence-id-235217

    Enlarge

Showing: 3801 - 3850 of 5238 results