12,610 results

1. pMSCV-IRES-GFP-MD2-HA

   Plasmid

   #133065

   Purpose

   Retroviral expression of mouse MD2 tagged with HA

   Depositor

   Jonathan Kagan

   Article

   Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

   Insert

   MD2 (Ly96 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GluA2-scFv-mNeon-KDEL

   Plasmid

   #230057

   Purpose

   AAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA2 single chain variable fragment (scFv)
   

   Use

   AAV

   Tags

   ER retrieval motif (KDEL) and mNeon

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pv6_CBX1_2xChromo

   Plasmid

   #179393

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   CBX1_2xChromo
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDF-His-mTET1CD

   Plasmid

   #81053

   Purpose

   bacterial expression of the catalytic domain of murine TET1

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TET1 (Tet1 Mouse)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   catalytic domain amino acid 1367-2057

   Promoter

   T7

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-ninein C-ter

   Plasmid

   #73523

   Purpose

   Encodes the C-terminus of mouse ninein (residues 1874-2113) with an EGFP tag at its N-terminus

   Depositor

   Michel Bornens

   Article

   Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

   Insert

   ninein C-ter (residues 1874-2113) (Nin Mouse)
   

   Use

   Tags

   EGFP tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pFUSE-rIgG-Fc2-ICAM1

   Plasmid

   #156462

   Purpose

   Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

   Depositor

   Dirk Mielenz

   Article

   Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

   Insert

   Intercellular Adhesion Molecule 1 (Icam1 Mouse)
   

   Use

   Tags

   ICAM1-rFc

   Expression

   Bacterial and Mammalian

   Mutation

   endogenous signal peptide replaced with IL2 signa…

   Promoter

   hEF1-HTLC

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-mouse PKA-Calpha-mEGFP

   Plasmid

   #45521

   Depositor

   Haining Zhong

   Article

   Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

   Insert

   PKA catalytic subunit alpha tagged by mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV4Neo-murine IL-17RA-HA

   Plasmid

   #46859

   Purpose

   expresses mouse IL-17RA

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   IL-17RA (Il17ra Mouse)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pTRIP TRE-BI GFP10-RHOA/RBD-GFP11

   Plasmid

   #182232

   Purpose

   Inducible coexpression of GFP10-RHOA and RBD-GFP11 domain

   Depositor

   Stéphanie Cabantous

   Article

   Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

   Inserts

   ras homolog family member A (RHOA Human)
   rhotekin RHO binding domain (RBD)
   

   Use

   Lentiviral

   Tags

   GFP10 (split-GFP tripartite) and GFP11 (M4)

   Expression

   Mutation

   Promoter

   TRE bidirectional

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA4-FLAG-Jhdm2a-H1122A

    Plasmid

    #38140

    Depositor

    Toshinobu Nakamura, Toru Nakano

    Article

    Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

    Insert

    Jhdm2a-H1122A (Kdm3a Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    protein starts at aa115; H1122A

    Promoter

    CMV

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tet-O-FUW-Sox10

    Plasmid

    #45843

    Depositor

    Marius Wernig

    Article

    Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

    Insert

    Sox10 (Sox10 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. CLC-pmCherryC1

    Plasmid

    #27680

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Clathrin Light Chain (Clta Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHes1(467)-luc

    Plasmid

    #41723

    Depositor

    Ryoichiro Kageyama, Raphael Kopan

    Article

    Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

    Insert

    Hes1 Promoter (-467 to +46) (Hes1 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains the murine Hes1 promoter (-467 to +46)

    Promoter

    Hes1 promoter fragment (-467 to +46)

    Available since

    March 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT2-shP53

    Plasmid

    #124261

    Purpose

    Expresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.

    Depositor

    Maria Castro

    Article

    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

    Insert

    shP53
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. GluA2 (px330:2-guideCas9)

    Plasmid

    #169420

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pROSA26-PA

    Plasmid

    #21271

    Depositor

    Frank Costantini

    Article

    Srinivas et al BMC Dev Biol. 2001;1:4. doi: 10.1186/147

    Insert

    ROSA26 (Gt(ROSA)26Sor Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZac2.1-GfaABC1D -IgK-TurboID-HA

    Plasmid

    #219810

    Purpose

    Secreted HA-tagged TurboID for extracellular proximity labeling

    Depositor

    Cagla Eroglu

    Article

    Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

    Insert

    IgK (Igk Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mAIRN HO

    Plasmid

    #207411

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. mAIRN CD

    Plasmid

    #207412

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131461

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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