We narrowed to 11,193 results for: ENA
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Plasmid#163141PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only
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pLyGo-Bs-2
Plasmid#163140PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-3
Plasmid#163129PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-5
Plasmid#163131PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
YCplac33-NAT_NDT1
Plasmid#140593PurposeExpress NDT1 in S. cerevisiaeDepositorInsertNDT1 with 5' and 3' UTRs (YIA6 Budding Yeast)
ExpressionYeastPromoterNDT1 promoter and 3'UTRAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg2
Plasmid#113967PurposeSingle short guide RNA targeting TACCACATTTGTAGAGGTTDepositorInsertsg2
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg3
Plasmid#113968PurposeSingle short guide RNA targeting CAATGTATCTTATCATGTCDepositorInsertsg3
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
sg2+sg3
Plasmid#113970PurposeDouble short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTCDepositorInsertsg2+sg3
ExpressionMammalianPromoterU6Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A-SRDX
Plasmid#129682PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b-SRDX
UseCRISPR; Gateway compatible aaccas12b entry cloneExpressionPlantMutationD570A; AacCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-D573A-SRDX
Plasmid#129683PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b-SRDX
UseCRISPR; Gateway compatible bthcas12b entry cloneExpressionPlantMutationD573A; BthCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-T2-OgNLuc
Plasmid#135951PurposeExpresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.DepositorInsertOgNLuc LumiFluor
TagsT2 mitochondrial localization sequenceExpressionMammalianPromoterEF1aAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAWH-IRP
Plasmid#133896PurposePositive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22HDepositorInsertIRP
ExpressionYeastPromoterT7Available SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY151
Plasmid#130947PurposepspFΔHTH::λN22plus gene controlled by promoter PrhaBDepositorInsertsrhaS
pspFΔHTH::λN22plus
UseSynthetic BiologyTagspspFΔHTH::λN22plusExpressionBacterialMutationThe HTH domain of the pspF gene was deleted (297-…PromoterPcon and PrhaBAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/HP + GUG-nLuc-3XFLAG
Plasmid#127323PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with hairpin in 5' UTRDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AUU-nLuc-3XFLAG
Plasmid#127305PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUU start codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AAA-nLuc-3XFLAG
Plasmid#127306PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)DepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG(30)-FFLuc-3XFLAG
Plasmid#127336PurposeExpress truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 30DepositorInsertFirefly luciferase
Tags3xFLAGExpressionMammalianPromoterSV40Available SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/AGG-nLuc-3XFLAG
Plasmid#127304PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AGG start codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/ACG-nLuc-3XFLAG
Plasmid#127303PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from ACG start codonDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/GGG-nLuc-3XFLAG
Plasmid#127307PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)DepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4.13/AUG(59)-FFLuc-3XFLAG
Plasmid#127337PurposeExpress truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 59DepositorInsertFirefly luciferase
Tags3xFLAGExpressionMammalianPromoterSV40Available SinceJune 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXW109ABS62(RS)
Plasmid#123153PurposeAmplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS62-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR-ABS62, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXW109ABS67(RS)
Plasmid#123154PurposeAmplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS67-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109. ParsR-ABS67, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Theo-LUCID
Plasmid#119048PurposePlasmid encoding the mammalian expression of the Theophiline LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Theo-LUCID_part1
Fab_Theo-LUCID_part2
TagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_Quin-LUCID
Plasmid#119049PurposePlasmid encoding the mammalian expression of the Quinine LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_Quin-LUCID_part1
Fab_Quin-LUCID_part2
TagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO-Fab_MTX-LUCID
Plasmid#119047PurposePlasmid encoding the mammalian expression of the Metotraxate LUCID based on antibodies (BRET semi-synthetic biosensor)DepositorInsertsFab_MTX-LUCID_part1
Fab_MTX-LUCID_part2
TagsFLAG-tag, His-tag, and Strep-tagExpressionMammalianPromoterEF-1α + CMV/SV40 enhancers and IRESAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0075
Plasmid#117642PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_Flavin-binding monooxygenase family protein {AT1G62600}(pEPOR1CB0098)and sgRNA_Flavin-binding monooxygenase family protein {AT1G62590}(pEPOR1CB0109)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0109
Plasmid#117571PurposeLevel1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62590} in A. thalianaDepositorInsertPromoter_AtU6-26 + sa_gRNA_Flavin-binding monooxygenase family protein {AT1G62590} A. thaliana
ExpressionPlantPromoterAtU6-26 (pICSL90002)Available SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0098
Plasmid#117570PurposeLevel1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62600} in A. thalianaDepositorInsertPromoter_AtU6-26 + sa_gRNA_Flavin-binding monooxygenase family protein {AT1G62600} A. thaliana
ExpressionPlantPromoterAtU6-26 (pICSL90002)Available SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK-ENH_BsmB1-MCS-Avi
Plasmid#110205PurposeRecipient vector for expression of C-terminally Avi-tagged protein of interest in chick embryos under the control of enhancer of choiceDepositorInsertENH_BsmB1-MCS-Avi
TagsAvi_tagExpressionBacterialAvailable SinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK-ENH_BsmB1-Avi-MCS-2A-Citrine
Plasmid#110204PurposeRecipient vector for expression of N-terminally Avi-tagged protein of interest in chick embryos under the control of enhancer of choice.DepositorInsertENH_BsmB1-Avi-MCS-2A-Citrine
TagsAvi_tag-2a-YFPExpressionBacterialAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CRISPR V2 sgMTHFD1L_2
Plasmid#106317PurposeExpress Cas9 and sgRNA targeting MTHFD1LDepositorInsertsgRNA targeting MTHFD1L
UseCRISPR and LentiviralExpressionMammalianAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)C
Plasmid#108194PurposeExpresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C)-P450cam fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+3(R112C/T180C)C
Plasmid#108193PurposeExpresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPCNA3(R112C/T180C)-P450cam fusion protein
TagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable SinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+2(L171C)X
Plasmid#108192PurposeExpresses PdR-PCNA2(L171C)-PdX fusion protein in E. coliDepositorInsertPCNA2(L171C)-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationL171C (PCNA2); C73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+R2(L171C)X
Plasmid#108191PurposeExpresses PdR-PCNA2(L171C)-PdX fusion protein in E. coliDepositorInsertPdR-PCNA2(L171C)-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationL171C (PCNA2); C73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+1(G108C)X
Plasmid#108189PurposeExpresses PCNA1(G108C)-PdX fusion protein in E. coliDepositorInsertPCNA1(G108C)-PdX fusion protein
TagsHis6 tagExpressionBacterialMutationG108C (PCNA1); C73S/C85S (PdX)PromoterT7 promoterAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-fsaA
Plasmid#102286PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.DepositorInsertfsaA gRNA
UseCRISPRExpressionBacterialPromoterpTetAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-adhE
Plasmid#102290PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.DepositorInsertadhE gRNA
UseCRISPRExpressionBacterialPromoterpTetAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-ompT
Plasmid#102291PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.DepositorInsertompT gRNA
UseCRISPRExpressionBacterialPromoterpTetAvailable SinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G10-R20
Plasmid#101819PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyPromoterSpy 1049Available SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G14-R20
Plasmid#101821PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyPromoterSpy 1049Available SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRRNA-G10-R11
Plasmid#101809PurposeRepress mCherry and GFP independently, with different strengthsDepositorInsertcrRNAs targeting GFP and mCherry
UseCRISPR and Synthetic BiologyPromoterSpy 1049Available SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(R37P)-RagC
Plasmid#99706PurposeBacterial expression of RagA(R37P)-RagCDepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationR37PPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(L91P)
Plasmid#99707PurposeBacterial expression of RagA-RagC(L91P)DepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationL91PPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(K84T)
Plasmid#99708PurposeBacterial expression of RagA-RagC(K84T)DepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationK84TPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA(R37P)-RagC
Plasmid#99646PurposeBacterial expression of RagA(R37P)-RagCDepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC(K84T))
Plasmid#99648PurposeBacterial expression of RagA-RagC(K84T)DepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA(T21N)-RagC
Plasmid#99650PurposeBacterial expression of RagA(T21N)-RagCDepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationT21NAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK RagA-RagC(S75N)
Plasmid#99699PurposeBacterial expression of RagA-RagC(S75N)DepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationS75NAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only