We narrowed to 2,101 results for: CO

1. pcDNA5-EGFP-NLS-P2A-mCherry-PTS1

   Plasmid

   #87803

   Purpose

   Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.

   Depositor

   Andrea Musacchio

   Article

   Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

   Inserts

   NLS
   PTS1
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter, tetracycline operator

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEM-Cas9HF1-indRecA56

   Plasmid

   #102294

   Purpose

   Modified from pEM-Cas9HF1 (Addgene ID: 89961) to co-express inducible recA56 to block recA-mediated double-strand break repair.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Inserts

   Cas9HF1
   recA56
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   N497A/R661A/Q695A/Q926A

   Promoter

   pTet

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-miRFP670nano3-Blast-H2BC11

   Plasmid

   #207762

   Purpose

   Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pBS-AMVpl-β1WT-nAchR

   Plasmid

   #97080

   Purpose

   To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

   Depositor

   Henry Lester

   Article

   Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

   Insert

   Beta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-moxGFP-Zeo-H2BC11

   Plasmid

   #207759

   Purpose

   Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCMV-PE2-tagRFP-BleoR

   Plasmid

   #192508

   Purpose

   Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

   Depositor

   Gerald Schwank

   Article

   Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

   Insert

   PE2-P2A-tagRFP-P2A-BleoR
   

   Use

   Tags

   BleoR, SV40 bpNLS, and tagRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFL-EGFP-3xFlag-DEST

   Plasmid

   #134903

   Purpose

   Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Type

   Empty backbone

   Use

   Tags

   3x-Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedron

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

   Plasmid

   #105043

   Purpose

   Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Stk11 (Stk11 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-SynI-CreOn-FlpOff-mNaChBacMUT-P2A-EGFP

   Plasmid

   #176281

   Purpose

   Viral vector for co-expression of non-functional NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   mNaChBacMUT-P2A-EGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   E191K

   Promoter

   human Synapsin I

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-N-Solo-mStayGold-MYO1C

    Plasmid

    #227306

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid

    #87828

    Purpose

    High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter, tetracycline operator

    Available since

    April 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. NEK7_HUMAN_D0

    Plasmid

    #79735

    Purpose

    This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    NEK7 (NEK7 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3

    Plasmid

    #121976

    Purpose

    transfection studies in eukaryotic cells such as COS-7 cells

    Depositor

    Christophe Erneux

    Article

    De Smedt et al J Biol Chem. 1996 Apr 26;271(17):10419-2

    Insert

    Type I Inositol-1,4,5-trisphoosphate 5-phosphatase (INPP5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSF-Ivy-his-hLYZ

    Plasmid

    #83920

    Purpose

    Co-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Ivy
    lysozyme (LYZ Human)
    

    Use

    Tags

    Ivy protein has C-terminal hisx6 tag

    Expression

    Bacterial

    Mutation

    Ivy leader sequence deleted and replaced.

    Promoter

    T7 and t7

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-SynI-FlpOn-CreOff-MCS-P2A-mCherry

    Plasmid

    #176277

    Purpose

    Viral vector for co-expression of a CDS of interest and mCherry in cells expressing Flp AND NOT Cre driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.

    Depositor

    Martin Riccomagno

    Article

    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    MCS-P2A-mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin I

    Available since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lenti-SAM-hygro

    Plasmid

    #138955

    Purpose

    Lentiviral vector for co-expressing spCas9, dRNA-MS2, p65 and HSF1 fused to MS2 binding protein, and hygromycin resistance gene

    Depositor

    Nadya Dimitrova

    Article

    Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 AND EF1a

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBacDual with LFn + Needle

    Plasmid

    #84868

    Purpose

    Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    Needle
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG HDAC5 S259A/S498A

    Plasmid

    #32216

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser259Ala, Ser498Ala

    Promoter

    CMV

    Available since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid

    #87827

    Purpose

    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

    Depositor

    Andrea Musacchio

    Article

    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

    Inserts

    NLS
    PTS1
    

    Use

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter, tetracycline operator

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6gRNA5(Ptpn23-g1)-PGKpuroBFP-W

    Plasmid

    #105034

    Purpose

    Lentiviral gRNA plasmid targeting mouse Ptpn23 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Ptpn23 (Ptpn23 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge