We narrowed to 1,219 results for: cas12

Showing: 281 - 320 of 1219 results

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pCI108-SiT-Cas12a-[Cond]

Plasmid

#128407

Purpose

Conditionally express both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCE046-SiT-Cas12a-[Repr]

Plasmid

#128132

Purpose

Express both AsCas12a fused to [Repr] repressor domain and CRISPR arrays on a single transcript

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-[Repr]-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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U6-AsCas12f-full-length-sgRNA

Plasmid

#204638

Purpose

Expression of full-length AsCas12f sgRNA in mammalian cells

Depositor

Weixin Tang

Article

Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

Insert

full-length AsCas12f sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-AsCas12a-Plus-mCherry

Plasmid

#191231

Purpose

To express the AsCas12a nuclease variant "AsCas12a-Plus" that enhanced the editing activity and specificity

Depositor

Zhili Rong

Article

Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

Insert

AsCas12a-Plus-P2A-mCherry

Use

CRISPR

Mutation

E174R, R951K, R955A

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-enCas12a-A3A-Y130F

Plasmid

#193652

Purpose

Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-hA3A-Y130F, EGFP

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-Y132D

Plasmid

#193641

Purpose

Expression of base editor dCas12a-A3A-Y132D in mammalian cells for base editing

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-Y132D

Expression

Mammalian

Mutation

hAPOBEC3A-Y132D

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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TwinStrep-SUMO-NLS-BhCas12b_v4

Plasmid

#158615

Purpose

For protein expression of BhCas12b_v4

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

NLS-BhCas12b_v4

Tags

6xHIS-TwinStrep-SUMO

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCas-pML-1B_6xHis-Cas12m

Plasmid

#192282

Purpose

MmCas12m fused to His-tag for protein purification

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmCas12m

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCH4 (denAsCas12a-KRAB lenti)

Plasmid

#217331

Purpose

Lentiviral expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

D908A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCH68 (dAsCas12a-3xKRAB piggyBac)

Plasmid

#217334

Purpose

piggyBac expression of dAsCas12a-3xKRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

dAsCas12a

Use

CRISPR

Tags

nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

Expression

Mammalian

Mutation

E993A

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCI118-SiT-Cas12a-[Ind]

Plasmid

#128406

Purpose

Inducible expression of both AsCas12a and CRISPR arrays on single transcripts

Depositor

Randall Platt

Article

Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

Insert

AsCas12a-Triplex

Use

AAV

Expression

Mammalian

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-ABE8e-V106W

Plasmid

#193646

Purpose

Expression of base editor dCas12a-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e-V106W, EGFP

Expression

Mammalian

Mutation

TadA*8e-V106W

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)

Plasmid

#160611

Purpose

Transcriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

P35s:LbCas12a:T35s

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-FNLDTA

Plasmid

#195340

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis Tag and MBP, TEV cleavage site, 6x His Tag

Expression

Bacterial

Mutation

F208W, N524V, L795I, D868V, T874S, A1015E

Promoter

T7 Promoter

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pZ148-BhCas12b-sgRNA-scaffold

Plasmid

#122448

Purpose

Expresses sgRNA for BhCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BhCas12b-sgRNA-scaffold, CMV-mCherry

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1-v5.1+T1-empty

Plasmid

#204977

Purpose

AsCas12f1-v5.1+T1-based Human cell genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nature Catalysis (2023) 6, 695–709

Type

Empty backbone

Use

CRISPR

Mutation

N70Q/K103R/A104R/S118A/D364R

Promoter

PCMV,U6

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-AsCas12f-2NLS

Plasmid

#171613

Purpose

NLS-AsCas12f1-NLS heterologous expression plasmid for RNP

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

NLS-AsCas12f1-NLS

Expression

Bacterial

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCH62 (denAsCas12a-KRAB piggyBac)

Plasmid

#217333

Purpose

piggyBac expression of denAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

denAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mammalian

Mutation

D908A/E174R/S542R/K548R

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-N57G

Plasmid

#193639

Purpose

Expression of base editor dCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-N57G, EGFP

Expression

Mammalian

Mutation

hAPOBEC3A-N57G

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-Y130F

Plasmid

#193640

Purpose

Expression of base editor dCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-Y130F, EGFP

Expression

Mammalian

Mutation

hAPOBEC3A-Y130F

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pZ147-BvCas12b-sgRNA-scaffold

Plasmid

#122447

Purpose

Expresses sgRNA for BvCas12b in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

U6-BvCas12b-sgRNA-scaffold, CMV-mCherry

Expression

Mammalian

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-AsCas12a_crRNA-site4 (MSP3511)

Plasmid

#160139

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-opCas12a-Ultra-6xNLS

Plasmid

#209024

Purpose

Lentiviral vector expressing human codon-optimized opCas12a-Ultra with 6xNLS

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

opCas12a-Ultra-6xNLS-Flag-Myc-NeoR

Use

CRISPR and Lentiviral

Tags

FLAG and MYC

Expression

Mammalian

Mutation

E174R/M537R/S542R/F870L

Promoter

EF-1a

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-LbCas12a-Plus-mCherry

Plasmid

#191230

Purpose

To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

Depositor

Zhili Rong

Article

Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

Insert

LbCas12a-Plus-P2A-mCherry

Use

CRISPR

Mutation

D156R, R883K, R887A

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
2xNLS-NLP-cMyc LbaCas12a

Plasmid

#182123

Purpose

pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

Depositor

Scot Wolfe

Article

Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

Insert

2xNLS-NLP-cMyc LbaCas12a

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

Plasmid

#136381

Purpose

CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b (D570A)-TV-T2A-MCP-TV

Use

CRISPR

Expression

Plant

Mutation

D570A

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-AaCas12b(R911A)-2AeGFP

Plasmid

#121961

Purpose

Mammalian expression, Transcription regulation, deactivated AaCas12b

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

Catalytically deactivated AaCas12b

Tags

3xHA tag

Expression

Mammalian

Mutation

R911A

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGB3 alpha1 P35S:AsCas12a:T35S (GB1440)

Plasmid

#160610

Purpose

Transcriptional unit for the expression of the AsCas12a in plant systems driven by the CaMV35s promoter.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

P35s:AsCas12a:T35s

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-AsCas12a_crRNA-site3 (RTW549)

Plasmid

#160138

Purpose

T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-AaCas12b(D977A)-2AeGFP

Plasmid

#121962

Purpose

Mammalian expression, Transcription regulation, deactivated AaCas12b

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

Catalytically deactivated AaCas12b

Tags

3xHA tag

Expression

Mammalian

Mutation

D977A

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUPD2 AtU6-26:LbCas12a_DR (GB1443)

Plasmid

#160575

Purpose

LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AtU6-26:LbCas12a_DR

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Promoter

U6-26

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM2_Euk_2NLS-RVQ (LbCas12a)-2NLS

Plasmid

#244837

Purpose

Mammalian expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 2xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a-RVQ

Use

CRISPR

Tags

2xSV40 NLS

Expression

Mammalian

Mutation

G146R/R182V/E795Q

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM5_CL7_Bac_2NLS-Lbm6 (LbCas12a)-4NLS

Plasmid

#244840

Purpose

Bacterial expression of Lbm6 LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Lbm6-Cas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Mutation

D535G/S551F/D665N

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SM470_EF1A-dEnAsCas12a-T2A-EGFP

Plasmid

#244222

Purpose

Mammalian expression of dEnAsCas12a with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM6_CL7_Bac_2NLS-RVQ (LbCas12a)-2NLS

Plasmid

#244841

Purpose

Bacterial expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a-RVQ

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 2xSV40 NLS

Expression

Bacterial

Mutation

G146R/R182V/E795Q

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SM468_EF1A-dHyperLbCas12a-T2A-EGFP

Plasmid

#244220

Purpose

Mammalian expression of dHyperLbCas12a with EGFP selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SM471_EF1A-dEnAsCas12a-T2A-BlastR

Plasmid

#244223

Purpose

Mammalian expression of dEnAsCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dEnAsCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dEnAsCas12a: E174R, S542R, K548R, and D908A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SM469_EF1A-dHyperLbCas12a-T2A-BlastR

Plasmid

#244221

Purpose

Mammalian expression of dHyperLbCas12a with Blasticidin resistance selectable marker

Depositor

Tim Reddy

Article

Melore et al bioRxiv [Preprint]. 2024 Jul 9:2024.07.0

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pNS49 (enAsCas12a-KRAB piggyBac)

Plasmid

#217335

Purpose

piggyBac expresion of enAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

enAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mammalian

Mutation

E174R/S542R/K548R

Promoter

CAG

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-enCas12a-A3A-N57G

Plasmid

#193651

Purpose

Expression of base editor enCas12a-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLb-enCas12a-hA3A-N57G, EGFP

Expression

Mammalian

Mutation

dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-N57G

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 1,219 results for: cas12