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We narrowed to 1,494 results for: U6 promoter

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Showing: 351 - 400 of 1494 results

  2. pCRISPR/eCas9-GPIBY55

    Plasmid
    #213709
    Purpose
    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.
    Depositor
    Qilai Huang
    Article
    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:
    Insert
    EGFP
    Use
    CRISPR
    Tags
    3XFLAG and Twin-strep-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213709-plasmid-map-sequence-id-422949

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  3. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid
    #68356
    Purpose
    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68356-plasmid-map-sequence-id-119628

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  7. plenti guide Thy1.1

    Plasmid
    #128063
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Thy1.1 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128063-plasmid-map-sequence-id-255154

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  8. pJRH051

    Plasmid
    #171625
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF1-a and U6
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    171625-plasmid-map-sequence-id-335505

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  9. ATP1A1_G8_Dual_sgRNA

    Plasmid
    #178104
    Purpose
    Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178104-plasmid-map-sequence-id-350193

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  11. ATP1A1_G4_Q118R_EBFP_to_EGFP_Dual_pegRNA

    Plasmid
    #178100
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and EBFP_To_EGFP pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + EBFP to EGFP pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178100-plasmid-map-sequence-id-350130

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  14. pUb-Cas9-EGFP_cU6:6-sgRNA

    Plasmid
    #190597
    Purpose
    The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    EGFP - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190597-plasmid-map-sequence-id-379952

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  15. pAAV-shCTRL

    Plasmid
    #181875
    Purpose
    Expresses a control shRNA with no known targets in the mouse genome under control of the U6 promoter and mCherry under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    control shRNA with no known targets in the mouse genome
    Use
    AAV, Adenoviral, and Mouse Targeting
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    181875-plasmid-map-sequence-id-355955

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  16. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid
    #190598
    Purpose
    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter
    Depositor
    Claudia Rueckert
    Article
    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Tags
    mCherry - separated by T2A
    Promoter
    Aedes aegypti polyubiquitin and Culex quinquefasc…
    Available Since
    Jan. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    190598-plasmid-map-sequence-id-379953

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  17. ATP1A1_G2_Dual_sgRNA

    Plasmid
    #173201
    Purpose
    Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G2 sgRNA + user-specified sgRNA
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173201-plasmid-map-sequence-id-339508

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  19. pIK208

    Plasmid
    #65630
    Purpose
    sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"
    Depositor
    Iskra Katic
    Article
    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0
    Insert
    U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
    Use
    CRISPR
    Expression
    Worm
    Available Since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    65630-plasmid-map-sequence-id-111541

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  20. pLenti_dCas9-2xAM_hIRF-1

    Plasmid
    #92221
    Purpose
    Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter
    Depositor
    Hodaka Fujii
    Article
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Inserts
    Sp-dCas9-2xAM tag
    gRNA targeting human IRF-1 promoter
    Use
    CRISPR and Lentiviral
    Tags
    2xAM tag
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    CBh and U6
    Available Since
    June 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    92221-plasmid-map-sequence-id-181086

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  21. ATP1A1_G8_MTOR_Nick_Exon-53_Dual_sgRNA

    Plasmid
    #178107
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 + MTOR nick exon-53 sgRNAs
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178107-plasmid-map-sequence-id-350194

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  22. ATP1A1_G6_T804N_MTOR_E2419K_Dual_pegRNA

    Plasmid
    #178110
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-E2419K pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA + MTOR-E2419K pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178110-plasmid-map-sequence-id-350136

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  24. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

    Plasmid
    #173214
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
    Use
    CRISPR; Prime editing (pe3)
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173214-plasmid-map-sequence-id-339507

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  30. ATP1A1_G3_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid
    #178096
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 + MTOR nick exon-44 sgRNAs
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178096-plasmid-map-sequence-id-350135

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  31. ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid
    #178099
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 + MTOR nick intron-45 sgRNAs
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178099-plasmid-map-sequence-id-350138

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  32. ATP1A1_G4_Q118R_MTOR_F2108L_Dual_pegRNA

    Plasmid
    #178102
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-F2108L pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + MTOR-F2108L pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178102-plasmid-map-sequence-id-350124

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  33. ATP1A1_G4_Q118R_MTOR_I2017T_Dual_pegRNA

    Plasmid
    #178103
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-I2017T pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + MTOR-I2017T pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178103-plasmid-map-sequence-id-350190

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  34. ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid
    #178105
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 + MTOR nick exon-44 sgRNAs
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178105-plasmid-map-sequence-id-350131

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  35. ATP1A1_G8_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid
    #178106
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 + MTOR nick intron-45 sgRNAs
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178106-plasmid-map-sequence-id-350189

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  36. ATP1A1_G6_T804N_MTOR_I2017T_Dual_pegRNA

    Plasmid
    #178108
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-I2017T pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA + MTOR-I2017T pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178108-plasmid-map-sequence-id-350191

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  37. ATP1A1_G6_T804N_MTOR_F2108L_Dual_pegRNA

    Plasmid
    #178109
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 T804N-G6 and MTOR-F2108L pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA + MTOR-F2108L pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178109-plasmid-map-sequence-id-350192

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  38. ATP1A1_G4_Q118R_MTOR_E2419K_Dual_pegRNA

    Plasmid
    #173209
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-E2419K pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + MTOR E2419K pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173209-plasmid-map-sequence-id-339524

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  39. pSLQ1371-sgYAP-10

    Plasmid
    #121424
    Purpose
    sgYAP-10 sequence: GTGCTGTCCCAGATGAACGTC. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.
    Depositor
    Ovijit Chaudhuri, Stanley Qi
    Article
    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:
    Insert
    sgYAP-10 (GTGCTGTCCCAGATGAACGTC)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    mouse U6
    Available Since
    April 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    121424-plasmid-map-sequence-id-364370

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  41. plenti guide ds red

    Plasmid
    #128055
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and DsREd from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128055-plasmid-map-sequence-id-255092

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  42. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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  43. pKLV2-U6gRNA5(sgBart10-5p)-PGKpuro2ABFP-W

    Plasmid
    #252920
    Purpose
    Expression vector for gRNA with Puro and tagBFP selection markers
    Depositor
    Sok Ching Cheong
    Insert
    EBV-miR-Bart10-5p
    Use
    CRISPR
    Promoter
    human U6 promoter, mouse PGK promoter
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    252920-plasmid-map-sequence-id-505089

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  44. lentiCRISPRv2_PROX1_1+4

    Plasmid
    #121177
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    March 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    121177-plasmid-map-sequence-id-237896

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  45. gRNA_Cloning Vector BbsI

    Plasmid
    #128433
    Purpose
    Embty backbone vector for expression of gRNA for spCas9 genome editing or epigenome editing
    Depositor
    Izuho Hatada
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    U6 promoter
    Available Since
    July 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    128433-plasmid-map-sequence-id-251645

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  46. pUC19-U6_AluJb

    Plasmid
    #240314
    Purpose
    Overexpress the consensus AluJb retrotransposon sequence from a Pol-III promoter
    Depositor
    Bérénice Benayoun
    Insert
    AluJB
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240314-plasmid-map-sequence-id-477824

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  47. pX333

    Plasmid
    #64073
    Purpose
    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.
    Depositor
    Andrea Ventura
    Article
    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    3XFLAG-Cas9
    Expression
    Mammalian
    Promoter
    CBh; U6
    Available Since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    64073-plasmid-map-sequence-id-360030

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  48. ATP1A1_G6_T804N_Dual_pegRNA

    Plasmid
    #173200
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA
    Use
    CRISPR; Prime editing
    Expression
    Mammalian
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173200-plasmid-map-sequence-id-339515

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Showing: 351 - 400 of 1494 results