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pMSCV PIG (Puro IRES GFP empty vector)

Plasmid

#21654

Purpose

Retroviral backbone for cloning and gene expression. Select with puromycin or GFP.

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

GFP and IRES

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-RhoA-T19N

Plasmid

#12967

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA DN (RHOA Human)

Tags

EGFP

Expression

Mammalian

Mutation

T19N Dominant negative

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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myc-mTOR

Plasmid

#1861

Depositor

David Sabatini

Article

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

mTOR (Mtor Rat)

Tags

Myc

Expression

Mammalian

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

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pBABEpuro HA Brca1

Plasmid

#14999

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

Brca1 (BRCA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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HDAC6 Flag

Plasmid

#13823

Purpose

Mammalian expression of human histone deacetylase 6 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC6 (HDAC6 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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PGC-1 alpha promoter 2kb luciferase

Plasmid

#8887

Depositor

Bruce Spiegelman

Article

Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

Insert

PGC-1 alpha promoter (Ppargc1a Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti6-H2B-mCherry

Plasmid

#89766

Purpose

Lentivirus destination vector for stable expression of mCherry-tagged histone H2B

Depositor

Torsten Wittmann

Article

Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

Insert

Histone H2B (H2BC11 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEF1a-hMLH1dn

Plasmid

#174824

Purpose

Mammalian expression of human MLH1dn (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized)

Expression

Mammalian

Mutation

deletion of residues 754-756

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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3xAP1pGL3 (3xAP-1 in pGL3-basic)

Plasmid

#40342

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

3xAP-1

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains three canonical AP-1 binding sites (TGAC…

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-FLNa WT

Plasmid

#8982

Depositor

John Blenis

Article

Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

Insert

Filamin A (FLNA Human)

Tags

myc

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pdCas9-humanized

Plasmid

#44246

Purpose

Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

Depositor

Stanley Qi

Article

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dead Cas9 with 3X NLS

Use

CRISPR and Retroviral; Vector

Tags

3x NLS

Expression

Mammalian

Mutation

D10A H840A (catalytically inactive)

Promoter

MSCV LTR promoter

Available Since

April 8, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-ERK1

Plasmid

#14747

Depositor

Rony Seger

Article

Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

Insert

ERK1 (MAPK3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

June 12, 2007

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Tet1CD

Plasmid

#84475

Purpose

Lentiviral construct to express dCas9 fused with the catalytic domain of Tet1

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Tet1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-VSVG

Plasmid

#11912

Depositor

Jennifer Lippincott-Schwartz

Article

Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

Insert

VSVG

Expression

Mammalian

Mutation

F64L, S65T, A206K relative the wild type GFP sequ…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pHAGE EF1α dCas9-KRAB

Plasmid

#50919

Purpose

Constitutive dCas9-KRAB 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1alpha

Available Since

March 28, 2014

Availability

Academic Institutions and Nonprofits only

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Rictor_1 shRNA

Plasmid

#1853

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

Rictor (RICTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 10, 2005

Availability

Academic Institutions and Nonprofits only

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pMSCV-LTR-dCas9-VP64-BFP

Plasmid

#46912

Purpose

Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, VP64 and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

dCas9-VP64-BFP fusion
Puromycin resistance

Use

CRISPR and Retroviral

Tags

3xNLS, BFP, and VP64 domain

Expression

Mammalian

Promoter

LTR and PGK

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1-Q61L

Plasmid

#12981

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 constitutively active (RAC1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Q61L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-RhoA-wt

Plasmid

#12965

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

RhoA (RHOA Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-SIRT1 H363Y

Plasmid

#1792

Depositor

Michael Greenberg

Article

Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

Insert

SIRT1 (SIRT1 Human)

Tags

Flag

Expression

Mammalian

Mutation

H363Y, deacetylase domain mutation

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only

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