We narrowed to 2,368 results for: pcas9

1. pMA-SpCas9-g9

   Plasmid

   #80792

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMA-SpCas9-g8

   Plasmid

   #80791

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9 iFE

   Plasmid

   #190138

   Purpose

   Expresses SpCas9-POLB (iFE)

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9-POLB (HiFi iFE)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGGB-SpCas9

   Plasmid

   #210533

   Purpose

   Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   SpCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

   Plasmid

   #110300

   Purpose

   Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

   Depositor

   Chris Kok-Lung Chan

   Article

   Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

   Insert

   p53-binding protein 1 (TP53BP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pF(UG) hSyn spCas9

   Plasmid

   #179725

   Purpose

   human synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITI

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   spCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-T3-SpCAS9-NG-pA

   Plasmid

   #131465

   Purpose

   Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

   Insert

   Codon optimized SpCas9-NG
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

   Plasmid

   #172225

   Purpose

   Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   sgRNA
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

   Plasmid

   #188541

   Purpose

   Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

   Depositor

   Darrell Kotton

   Article

   Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

   Insert

   gRNA
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Sept. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-nSpCas9-NG

    Plasmid

    #171692

    Purpose

    Expresses nSpCas9-NG in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    nSpCas9-NG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1…

    Promoter

    EF1a

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. KA2601_eSpCas9-G2P

    Plasmid

    #124203

    Purpose

    Expression of eSpCas9(1.1) and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-HA-dSpCas9

    Plasmid

    #92112

    Purpose

    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9
    

    Use

    CRISPR

    Tags

    HA tag and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Cbh

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpCas9 TadCBEb

    Plasmid

    #193832

    Purpose

    Express TadCBEb (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDb-SpCas9 D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9CR4-VQR

    Plasmid

    #120224

    Purpose

    pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015

    Depositor

    Christopher Reisch

    Insert

    SpCas9-VQR
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Mutation

    Mutations D1135V/R1335V/T1337R

    Promoter

    pTet

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMC-6-AtSpCas9-9

    Plasmid

    #197778

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AtSpCas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. 2X_pX458_pSpCas9(BB)-2A-GFP_NANOG_bKO

    Plasmid

    #172224

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the telomeric human NANOG CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. peSpCas9-J23109

    Plasmid

    #113152

    Purpose

    Plasmid constitutively expressing eSpCas9 protein

    Depositor

    Chong Zhang

    Article

    Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

    Insert

    eSpCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K810A & K1003A & R1060A

    Promoter

    Available Since

    Dec. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9 dFE

    Plasmid

    #190137

    Purpose

    Expresses SpCas9-T4pol (dFE)

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9-T4pol (dFE)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Topo_SpCas9.tracr_LbCas12a.DR

    Plasmid

    #155049

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. KA2706_eSpCas9-PGKPacdtkCh

    Plasmid

    #124204

    Purpose

    Expression of eSpCas9(1.1) and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge