8,810 results

1. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

   Plasmid

   #107942

   Purpose

   Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
   

   Tags

   3x HA and NLS (nucleoplasmin)

   Expression

   Mammalian

   Mutation

   E174R, S542R, K548R, and N282A

   Promoter

   CAG

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti_dCas9-KRAB-MeCP2

   Plasmid

   #122205

   Purpose

   Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Lentiviral

   Tags

   KRAB-MeCP2

   Expression

   Mammalian

   Mutation

   Cas9 (D10A, H840A)

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. ppyCAG_RNaseH1_WT

   Plasmid

   #111906

   Purpose

   Express RNASEH1 in mammalian cell.

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEIGW roGFP2-Orp1

   Plasmid

   #64993

   Purpose

   Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Lentiviral

   Tags

   roGFP2

   Expression

   Mammalian

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK036.TRE-Flpe-WPRE (Supernova)

   Plasmid

   #85007

   Purpose

   For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

   Depositor

   Takuji Iwasato

   Article

   Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

   Insert

   NLS-FLPe-WPRE
   

   Expression

   Mammalian

   Available Since

   Nov. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-NbALFA

   Plasmid

   #159987

   Purpose

   For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   mCherry-NbALFA
   

   Tags

   8histidine-HRV3C protease cleavage site

   Expression

   Bacterial

   Mutation

   3GS linker between mCherry and NbALFA

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-GFP

   Plasmid

   #160697

   Purpose

   Expresses GFP in mammalian cells

   Depositor

   Christine Mayr

   Article

   Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

   Insert

   GFP
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. gag-COM-pol

   Plasmid

   #211373

   Purpose

   Packaging plasmid for v3b PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-COM-pol
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVUT-tTR-KRAB

   Plasmid

   #11651

   Purpose

   Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Has Service

   Cloning Grade DNA

   Insert

   hUbiquitin C, GFP, tTR-KRAB, Tet-on
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT3TS-RfxCas13d-HA

    Plasmid

    #141320

    Purpose

    Plasmid to carry out IVT of RfxCas13d (human codon-optimized)

    Depositor

    Miguel Angel Moreno-Mateos, Ariel Bazzini

    Article

    Kushawah et al Dev Cell. 2020 Sep 28;54(6):805-817.e7.

    Insert

    Rfx-Cas13d
    

    Use

    CRISPR

    Tags

    HA

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAGM47523

    Plasmid

    #153221

    Purpose

    Level 0 zCas9i vector

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    SpCas9 with introns and 2 NLS
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti CMV/TO GFP-MDC1 (779-2)

    Plasmid

    #26285

    Depositor

    Eric Campeau

    Insert

    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA HA HP1 gamma

    Plasmid

    #24080

    Depositor

    Naoko Tanese

    Article

    Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

    Insert

    HP1 gamma (CBX3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pgRNA-CKB

    Plasmid

    #73501

    Purpose

    gRNA expression vector (with mKate2)

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    mKate2-T2A-Bsd
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC0046-EF1a-PspCas13b-NES-HIV

    Plasmid

    #103862

    Purpose

    Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    PspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and HIV NES

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAAV-CA

    Plasmid

    #69616

    Purpose

    To express gene strongly with AAV

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti_gRNA-Puro

    Plasmid

    #84752

    Purpose

    Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

    Plasmid

    #196702

    Purpose

    Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 wild type
    

    Expression

    Mammalian

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW57.1-FOXM1c

    Plasmid

    #68810

    Purpose

    Doxycycline inducible lentiviral vector for FOXM1c expression in mammalian cells

    Depositor

    Adam Karpf

    Article

    Barger et al Oncotarget. 2015 Jul 16.

    Insert

    FOXM1c (FOXM1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet ON

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-E-cadherin-RFP

    Plasmid

    #79603

    Purpose

    pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    CDH1 promoter region (CDH1 Human)
    

    Use

    Lentiviral

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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