Addgene

We narrowed to 6,032 results for: WUS;

Showing: 2371 - 2400 of 6032 results

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AV4-EF1-NMNAT2

Plasmid

#124970

Purpose

Express NMNAT2

Depositor

Kazuhiro Oka

Article

Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

Insert

NMNAT2, GFP (NMNAT2 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

EF1-alpha

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-8x

Plasmid

#172284

Purpose

Expressing EGFP mRNA fused with 8 tandem repeats of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-8x

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-SV40-Galpha-i1 ONE-GO

Plasmid

#204328

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-i1 ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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MTK4_001

Plasmid

#123810

Purpose

Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Bgh pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_032

Plasmid

#123995

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA1 -hAAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

Plasmid

#132409

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRanCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H147A; H1044A

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-SMVP-Cas9N-U6-gRNA TdL

Plasmid

#80938

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pSIN-Ski-gRNA2

Plasmid

#180369

Purpose

targeting mouse Ski gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Ski targeting gRNA (Ski Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-TAK1-K63A

Plasmid

#43933

Depositor

Bryant Darnay

Article

Besse et al J Biol Chem. 2007 Feb 9;282(6):3918-28.

Insert

Transforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

K63A

Promoter

Available since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_ASCL5_P2A_Hygro_Barcode

Plasmid

#120430

Purpose

Barcoded lentiviral vector to express ASCL5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL5 (ASCL5 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_033

Plasmid

#123996

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA2 -hAAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK234_034

Plasmid

#123997

Purpose

Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

sgRNA3 -hAAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCLXSN-Tpl2/Cot

Plasmid

#27558

Depositor

Shao-Cong Sun

Article

Babu et al J Biol Chem. 2006 May 19. 281(20):14041-

Insert

Tpl2/Cot (MAP3K8 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MKK4beta

Plasmid

#15517

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

pCDNA3 Flag MKK4beta (MAP2K4 Human)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pEGFP-C-SLC35B2

Plasmid

#155037

Purpose

Transient expression of EGFP-SLC35B2

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

SLC35B2 (SLC35B2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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MTK0_031

Plasmid

#123970

Purpose

Encodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BxBI-hygro-mRuby2PEST landing

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pKMV-VioC

Plasmid

#65349

Purpose

systhesis of vioC gene in the violacein pathway

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioC

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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POT4-RFP

Plasmid

#65320

Purpose

receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

Depositor

Junbiao Dai

Article

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pLexm-gfp-Pept2

Plasmid

#167988

Purpose

expresses rat PepT2 with a c terminal cleavable GFP-His tag.

Depositor

Simon Newstead

Article

Parker et al Sci Adv. 2021 Aug 25;7(35). pii: 7/35/ea

Insert

PepT2 (Slc15a2 Rat)

Use

Tags

cleavable GFP-his8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_POU1F1_P2A_Hygro_Barcode

Plasmid

#120473

Purpose

Barcoded lentiviral vector to express POU1F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

POU1F1 (POU1F1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac

Plasmid

#129685

Purpose

(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoter

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGex-3x MKK4

Plasmid

#14617

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
MTK4a_020

Plasmid

#123833

Purpose

Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d4PEST tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pQZ210

Plasmid

#106414

Purpose

Co-expresses trans SNARE complex with pDC183 in E.coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Inserts

Rat Synaptobrevin-2 fragment 28-66 (Vamp2 Synthetic)
Syntaxin-1A fragment 191-256 (Stx1a Rat, Synthetic)

Use

Tags

His tag

Expression

Bacterial

Mutation

Fragment 191-256 , Codon optimization and Fragmen…

Promoter

T7

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pTorPE-B-GECO1

Plasmid

#32469

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

B-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pQZ083

Plasmid

#106417

Purpose

Express Complexin-1 fragment (1-83) in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Insert

complexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)

Use

Tags

GST tag

Expression

Bacterial

Mutation

Fragment 1-83, Codon optimization

Promoter

T7

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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MTK4a_019

Plasmid

#123832

Purpose

Encodes the d2 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d2PEST tag

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pXZX352

Plasmid

#169456

Purpose

MATA fragment in pTOPO vector

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

Mata locus

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pXZX353

Plasmid

#169457

Purpose

MATα (alpha) fragment in pTOPO vector

Depositor

Jef Boeke

Article

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

MatA

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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MTK7_013

Plasmid

#123880

Purpose

Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RA_hAAVS1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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