-
Plasmid#40067DepositorInserthSlp4-a V21A (SYTL4 Human)
UseTagsGSTExpressionBacterialMutationdeleted SHD domain (1-143)PromoterTacAvailable sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
-
pcDNA4-Tet-on-vsv-Mis12-dCEN Incenp
Plasmid#108482Purposeinducible expression of vsv-Mis12-dCEN-INCENPDepositorInsertMis12-dCEN-INCENP (INCENP Human)
UseTagsVSVExpressionMammalianMutationCEN box replaced by Mis12PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-Tet-on-vsv-Mis12-dCEN-Incenp-GFP
Plasmid#108485Purposeinducible expression of vsv-Mis12-dCEN-INCENP-EGFPDepositorInsertMis12-dCEN-INCENP (INCENP Human)
UseTagsEGFP and VSVExpressionMammalianMutationCEN box replaced by Mis12PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRevTRE SGFP2 Inpp5E WT shRNA resistant
Plasmid#161997PurposeTet-responsive retroviral expression vector. Contains INPP5E. Also contains SGFP tag.DepositorInsertINPP5E (INPP5E Human)
UseRetroviralTagsSGFPExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo:β2-365AAQA368 L349M
Plasmid#35610DepositorInsertnAChR beta2 L349M 365AAQA368 (Chrnb2 Mouse, mouse strain 129)
UseTagsExpressionMammalianMutationL349M and 365AAQA368PromoterCMVAvailable sinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-HLA-A1-VN
Plasmid#135502PurposeMammalian expression of VN-fused and myc-tagged HLA-A*01:01DepositorInsertHLA-A*01:01 (HLA-A Human)
UseTagsExtracellular c-myc epitope tag, Signal peptide f…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Frt-V5-hspB9
Plasmid#63110PurposeExpression of HSPB9 (small HSP) tagged with V5 in mammalian cellsDepositorInserthspb9 (HSPB9 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-GEI-17(133-509)
Plasmid#87056PurposeExpresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORFDepositorInsertGEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)
UseTags6xHis-TEV and GAGGGS linkerExpressionBacterialMutationPromoterT7Available sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T-1 hSlp4-a V21A
Plasmid#40064DepositorInserthSlp4-a V21A (SYTL4 Human)
UseTagsGSTExpressionBacterialMutationValine 21 to AlaninePromoterTacAvailable sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Myc-TRFcM
Plasmid#44575DepositorUseRetroviralTagsMycExpressionMammalianMutationTRF2 DNA-binding domain (aa 444-500) is replaced …PromoterAvailable sinceMay 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE Flag HA ITPA
Plasmid#237998PurposeInducible lentiviral expression of ITPADepositorInsertITPA (ITPA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE Flag HA MPG
Plasmid#237804PurposeLentiviral vector expressing WT MPGDepositorInsertMPG (MPG Human)
UseTagsFlag 3HAExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE Flag HA MPG Catalytic Inactive
Plasmid#237807PurposeLentiviral vector expressing Catalytic Inactive MPGDepositorInsertMPG (MPG Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
p5E-Dll4-F2-E1B-B-Globin (JDW 1366)
Plasmid#229843PurposeA gateway compatible 5' entry clone containing the murine Dll4 F2 arterial specific enhancer upstream of a minimal E1b promoter and intron for arterial and endocardial expression.DepositorInsertDll4-F2 (exon3)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)
Plasmid#229811PurposeA Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)DepositorInsertLexA transactivator-LexA-Operon-nls-GFP
UseTol2 based expression vectorTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-nls-mCherry-stop-IRES-myr-EGFP (JDW 1326)
Plasmid#229812PurposeA CAGGS driven expression vector containing an nls-mCherry followed by an IRES and then a myristoylated EGFP for labeling the cell membrane.DepositorInsertnls-mCherry
UseTagsSV40 NLSExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)
Plasmid#229813PurposeA Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.DepositorInsertLexA-mODC-LexAOp-cFos
UseTol2 based expression vectorTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)
Plasmid#229817PurposeA gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.DepositorTypeEmpty backboneUseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Tet-Off-FLEX-DEST (JDW 1186)
Plasmid#229820PurposeAn AAV, tet-off, gateway compatible destination vector with cre dependent expression of the insert. EFS driving destablized rTADepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)
Plasmid#229823PurposeA CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.DepositorInsertLuciferase-P2A-H2A-mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)
Plasmid#229831PurposeA gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.DepositorTypeEmpty backboneUseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)
Plasmid#229838PurposeA Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.DepositorInsertLexA-mODC-LexAOp
UseTol2 based expression vectorTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLViP-LifeAct-TagRFPT
Plasmid#229700PurposeLentiviral expression of LifeAct-TagRFPT in mammalian cellsDepositorInsertSynthetic construct LifeAct-mTurquoise gene
UseLentiviralTagsTagRFPTExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLIX401 APE1 CI
Plasmid#227191PurposeInducible lentiviral expression of CI (Catalytically Inactive) APE1DepositorInsertapurinic/apyrimidinic endonuclease APE1 (APEX1 Human)
UseLentiviralTagsExpressionBacterial and MammalianMutationH309NPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanI-101
Plasmid#225065PurposeExpresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanI-101)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-EFS (EF1a core) (JDW 1319)
Plasmid#224474PurposeGateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.DepositorInsertEFS promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Luciferase-P2A-H2A-mCherry (JDW 926)
Plasmid#224488PurposeGateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)DepositorInsertLuciferase-P2A-H2A-mCherry
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)
Plasmid#224505PurposeGateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expressionDepositorInsertLexA-mODC-LexOP-35SCaMV
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-LexA-mODC-LexOP-c-Fos (JDW 1359)
Plasmid#224506PurposeGateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression constructDepositorInsertLexA-oMDC-LexOP-c-Fos
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)
Plasmid#224512PurposeA Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)DepositorInsert3xHA-BirA-T2A-mCherry-Caax
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)
Plasmid#224522PurposeA Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassetteDepositorInsertlox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroR
Plasmid#218916PurposeLentiviral construct expressing msfGFP and nuclear mCherry proteins.DepositorInsertEF1a-msfGFP-T2A-mCherry-NLS > mPGK-PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5(PrD)
Plasmid#166167PurposeGateway entry vector for N. furzeri DDX5-PrD(535-634) mutantDepositorInsertDDX5-PrD(535-634)
UseGateway entry vectorTagsExpressionMutationPrD only 1606-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5ΔPrD
Plasmid#166164PurposeGateway entry vector for N. furzeri DDX5ΔPrD(1-535) mutantDepositorInsertDDX5ΔPrD(1-535)
UseGateway entry vectorTagsExpressionMutationdeletion of 1606-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5ΔIDR
Plasmid#166165PurposeGateway entry vector for N. furzeri DDX5ΔIDR(1-483) mutantDepositorInsertDDX5ΔIDR(1-483)
UseGateway entry vectorTagsExpressionMutationdeletion of 1450-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-DDX5(IDR)
Plasmid#166166PurposeGateway entry vector for N. furzeri DDX5-IDR(483-634) mutantDepositorInsertDDX5-IDR(483-634)
UseGateway entry vectorTagsExpressionMutationIDR only 1450-1902PromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-mRuby3
Plasmid#166141PurposemRuby3 expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.DepositorInsertmRuby3
UseTagsExpressionYeastMutationPromoterGAL1Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB-SNAP
Plasmid#166146PurposeGateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.DepositorTypeEmpty backboneUseTagsHis10 and SNAPExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-ccdB
Plasmid#166147PurposeGateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli.DepositorTypeEmpty backboneUseTagsHis10-Smt3ExpressionBacterialMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only