166,169 results

1. mCherry-cGAS-HA

   Plasmid

   #231895

   Purpose

   Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   cGAS (CGAS Human)
   

   Tags

   3xHA and mCherry

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. LMAN2-bio-His

   Plasmid

   #51923

   Purpose

   Expresses full-length Vesicular integral-membrane protein VIP36 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   LMAN2 (LMAN2 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. ppyCAG_RNaseH1_D210N

   Plasmid

   #111904

   Purpose

   Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed D 210 to N

   Available Since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAVS-WT

   Plasmid

   #52135

   Purpose

   Retroviral vector for expression of human MAVS in mammalian cells

   Depositor

   Jonathan Kagan

   Article

   Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

   Insert

   MAVS (MAVS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET23b-15F11-HA-mEGFP-6xHis

   Plasmid

   #129593

   Purpose

   The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.

   Depositor

   Tim Stasevich

   Article

   Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

   Insert

   Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
   

   Tags

   6xHis-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-2xFLAG-SREBP-2

   Plasmid

   #26807

   Depositor

   Timothy Osborne

   Article

   Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

   Insert

   Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)
   

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   L7V, G76S, K395R and I482L

   Promoter

   CMV

   Available Since

   July 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. cytoplasmic cpVenus control

   Plasmid

   #186788

   Purpose

   Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   cytoplasmic cpVenus control
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMK1334

   Plasmid

   #127965

   Purpose

   sgRNA expression vector compatible with CROP-Seq and imaging screens

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Aug. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBR HPV31 9E

   Plasmid

   #221009

   Purpose

   Human papillomavirus 31 isolate 9E, complete genome cloned in pBR322

   Depositor

   Alison McBride

   Insert

   Human papillomavirus type 31 genome 9E isolate
   

   Use

   Cloning vector

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

    Plasmid

    #127289

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

    Inserts

    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and Ascl1 linked via 2A

    Expression

    Mammalian

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTXB1-sfGFP

    Plasmid

    #51562

    Purpose

    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    superfolder GFP
    

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB533-44B12m23-sfGFP

    Plasmid

    #186778

    Purpose

    RNAP2 Ser5ph-mintbody expression vector for mammalian cells

    Depositor

    Hiroshi Kimura

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    44B12m23-scFv
    

    Tags

    sfGFP

    Expression

    Mammalian

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC37A4

    Plasmid

    #131914

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC37A4 (SLC37A4 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. HSD11B1

    Plasmid

    #39155

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSD11B1 (HSD11B1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    T220I; codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pET-32a(+)-TGFB3

    Plasmid

    #120288

    Purpose

    Produce human recombinant TGFB3 in DE3 cells

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    Transforming Growth Factor-β3 (TGFB3 Synthetic, Human)
    

    Tags

    6x His and S-Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-TFR-20

    Plasmid

    #55144

    Purpose

    Localization: Transferrin Receptor, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TFR (TFRC Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMKO.1 GFP

    Plasmid

    #10676

    Purpose

    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

    Depositor

    William Hahn

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. L_NLS 41kDa

    Plasmid

    #201342

    Purpose

    Nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    (NLS)SV40A4-EGFP-2PrA
    

    Expression

    Mammalian

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-mKOR

    Plasmid

    #179285

    Purpose

    Express mouse kappa opioid receptor (GFP tagged) in mammalian cells

    Depositor

    Christian Gruber

    Article

    Muratspahic et al J Med Chem. 2021 Jul 8;64(13):9042-9055.

    Insert

    kappa opioid receptor (Oprk1 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pABE8e-protein

    Plasmid

    #161788

    Purpose

    Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

    Depositor

    David Liu

    Article

    Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

    Insert

    ABE8e base editor
    

    Tags

    8xHIS and BPNLS

    Expression

    Bacterial

    Promoter

    pRhaBAD

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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