166,169 results

1. shCTNNB1 # 2

   Plasmid

   #42544

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAX198

   Plasmid

   #173042

   Purpose

   Custom vector for sgRNA library construction

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. R777-E227 Hs.RASA1

   Plasmid

   #70511

   Purpose

   Gateway ORF clone of human RASA1 [NM_002890.2] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   RASA1 (RASA1 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF0769

   Plasmid

   #141692

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MSX1 (MSX1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-STIM1-Venus-173-C

   Plasmid

   #87619

   Purpose

   Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

   Depositor

   Jin Zhang

   Article

   Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

   Insert

   STIM1-Venus173-C (STIM1 Synthetic, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. shCTNNB1 # 1

   Plasmid

   #42543

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGagMBPHis

   Plasmid

   #42176

   Depositor

   James Hurley

   Article

   Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

   Insert

   Gag protein sequence is from HIV-1 clone NL4-3
   

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Available Since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pLEX307-TMPRSS4-puro

   Plasmid

   #158472

   Purpose

   Constitutive lentiviral expression of human TMPRSS4.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   TMPRSS4 (TMPRSS4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPCTαKN0005

   Plasmid

   #187569

   Purpose

   Transcriptional unit for constitutive expression of nanoluc luciferase driven by the 35s promoter

   Depositor

   Nicola Patron

   Article

   Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

   Insert

   Luciferase
   

   Expression

   Plant

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. plenti-As-Cas12a-2xNLS

    Plasmid

    #155047

    Purpose

    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    AsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS, Myc-tag and SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Venus-173-N-ORAI1

    Plasmid

    #87618

    Purpose

    N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus173-N-ORAI1 (ORAI1 Synthetic, Human)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pF hPGK mc2 IRES blast

    Plasmid

    #206239

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE-Tau-nYFP

    Plasmid

    #92204

    Purpose

    Retroviral overexpression vector for Tau bimolecular fluorescence complementation (BiFC)

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Retroviral

    Tags

    YFP(1-155)

    Expression

    Mammalian

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBT29-splitD

    Plasmid

    #122599

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. LSB-hsa-miR-30d-5p

    Plasmid

    #103417

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-30d-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-30d-5p target (MIR30D Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInducer-FRA1

    Plasmid

    #192267

    Purpose

    Doxycycline-inducible expression of murine FRA1 (Fosl1)

    Depositor

    Günter Schneider

    Article

    Schneeweis et al Cell Mol Life Sci. 2022 Dec 19;80(1):12.

    Insert

    Fosl1 (Fosl1 Mouse)
    

    Use

    Lentiviral; Inducible expression

    Expression

    Mammalian

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD649-HAsp-PILRA-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156577

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    PILRA (PIGR Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT29-splitC

    Plasmid

    #122598

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO-RFP-IKZF1-sh3

    Plasmid

    #69042

    Purpose

    Lentiviral expression of IKZF1 shRNA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(+) HIPK3 Sense

    Plasmid

    #60634

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA

    Depositor

    Jeremy Wilusz

    Article

    Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

    Insert

    Homeodomain-interacting protein kinase 3 (HIPK3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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