163,584 results

1. pAAV2-CAG-3xNLS-mScarlet

   Plasmid

   #191098

   Purpose

   To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

   Depositor

   Murray Blackmore, Pantelis Tsoulfas

   Article

   Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

   Insert

   3xNLS-mScarlet
   

   Use

   AAV and Synthetic Biology

   Tags

   Three repeats of the nuclear localizzation seque…

   Expression

   Mammalian

   Mutation

   Optimized to human codon usage

   Promoter

   CMV enhancer and CAG

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3/F-tractin=mStayGold

   Plasmid

   #212019

   Purpose

   For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   F-tractin
   

   Use

   Tags

   mStayGold

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA6-N-3XFLAG-Gsk3b

   Plasmid

   #123592

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   glycogen synthase kinase 3 beta (Gsk3b Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1(+)-CMV-bArrestin2-TEV

   Plasmid

   #107245

   Purpose

   Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   ARRB2
   

   Use

   Tags

   TEV(NIA), tobacco etch virus NIA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-beta-HA-BRCA2

   Plasmid

   #133553

   Purpose

   Mammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORF

   Depositor

   David Livingston

   Article

   Chen et al Mol Cell. 1998 Sep;2(3):317-28.

   Insert

   BRCA2 (BRCA2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK419 (GAL-OsTIR1(F74G))

   Plasmid

   #140656

   Purpose

   GAL-OsTIR1(F74G)

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   GAL-OsTIR1(F74G)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TAC-ERP2

   Plasmid

   #80478

   Purpose

   All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)

   Depositor

   Knut Woltjen

   Article

   Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-MMLVgag-3xNES-ABE8e

   Plasmid

   #181751

   Purpose

   MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE8e
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-PGK-Venus-Akaluc (neo)

   Plasmid

   #124701

   Purpose

   Aka-Luciferase reporter

   Depositor

   Roland Friedel

   Article

   Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

   Insert

   Venus-Akaluciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTP955

    Plasmid

    #104164

    Purpose

    Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF Synaptophysin-mRuby

    Plasmid

    #188980

    Purpose

    Synaptic buton marker

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Synaptophysin (Syp Synthetic, Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiCas9-EGFP

    Plasmid

    #63592

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang, Phil Sharp

    Article

    Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-DIO-FLPo

    Plasmid

    #118027

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-DIO-FLPo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-DIO-mCherry

    Plasmid

    #50462

    Purpose

    Double floxed mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. LbCpf1-2NLS

    Plasmid

    #102566

    Purpose

    bacterial expression of LbCpf1-2NLS

    Depositor

    Jennifer Doudna

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    LbCpf1
    

    Use

    Tags

    6xHis-MBP-TEV and HA-2xSV40NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPSU20-40-60-80

    Plasmid

    #173839

    Purpose

    Coexpresses 20, 40, 60 and 80 kD Penn State ladder proteins

    Depositor

    Song Tan

    Article

    Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

    Insert

    STRHSTPABPACCBPv3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTRKH3-slpGFP

    Plasmid

    #27168

    Depositor

    Michela Lizier

    Article

    Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

    Insert

    surface (S)-layer protein promoter region fused with modified green fluorescent protein 5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDMyc-TNPO1

    Plasmid

    #86081

    Purpose

    A mammalian expression plasmid encoding Transportin-1 (TNPO1) with N-terminus double Myc-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Transportin-1 (TNPO1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCXLE-gw

    Plasmid

    #37626

    Purpose

    Non-integrating (episomal) expression vector with Gateway cassette

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. Orai1 YFP

    Plasmid

    #19756

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    Orai1 (ORAI1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge