8,810 results

1. AAV-CAG-hChR2-H134R-tdTomato

   Plasmid

   #28017

   Purpose

   AAV-mediated expression of hChR2(H134R) for neuronal excitation.

   Depositor

   Karel Svoboda

   Article

   Mao et al Neuron. 2011 Oct 6;72(1):111-23.

   Has Service

   AAV Retrograde

   Insert

   humanized ChR2-H134R-tdTomato
   

   Use

   AAV; Adeno associate viral

   Tags

   tdTomato

   Mutation

   H134R

   Promoter

   CAG

   Available Since

   Nov. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.1(Hygro) myc-hPolQ-Flag

   Plasmid

   #73132

   Purpose

   To express in mammalian cells human PolQ

   Depositor

   Agnel Sfeir

   Article

   Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

   Insert

   Polymerase theta (POLQ Human)
   

   Tags

   Flag and myc

   Expression

   Mammalian

   Promoter

   cmv promoter

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. TLCV2-RB1

   Plasmid

   #87836

   Purpose

   LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Insert

   sgRB1 (RB1 Human)
   

   Use

   CRISPR and Lentiviral; Doxycycline inducible; egf…

   Expression

   Mammalian

   Promoter

   Tight TRE promoter and U6

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-AKAR4-Kras

   Plasmid

   #61621

   Purpose

   Non-raft/plasma membrane-targeted FRET biosensor for monitoring PKA activity

   Depositor

   Jin Zhang

   Article

   Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

   Insert

   AKAR4-Kras
   

   Tags

   C-terminal targeting sequence from Kras, Cerulean…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-ihSyn1-tTA

   Plasmid

   #99120

   Purpose

   An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Has Service

   AAV1

   Insert

   tTA
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   *(see below)

   Promoter

   ihSyn1

   Available Since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUGW-Pol2-ffLuc2-eGFP

   Plasmid

   #71394

   Purpose

   Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoter

   Depositor

   Glenn Merlino

   Article

   Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

   Insert

   Pol2-ffLuc2-eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Pol2 (mouse RNA polymerase II gene promtoer)

   Available Since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. PG13-luc (wt p53 binding sites)

   Plasmid

   #16442

   Purpose

   Luciferase reporter containing 13 copies of the p53-binding consensus sequence

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cell. 1993 Nov 19. 75(4):817-25.

   Insert

   p53-binding site (TP53 )
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCE-hOCT3/4

   Plasmid

   #41813

   Purpose

   Non-integrating (episomal) expression of human OCT3/4

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   POU5F1 (POU5F1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFPC1-huNFATc1EE-WT

   Plasmid

   #24219

   Depositor

   Jerry Crabtree

   Article

   Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

   Insert

   NFATc1 (NFATC1 Human)
   

   Tags

   EE and GFP

   Expression

   Mammalian

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. E1b-GFP-Tol2

    Plasmid

    #37845

    Depositor

    Nadav Ahituv

    Article

    Birnbaum et al Genome Res. 2012 Jun;22(6):1059-68. Epub

    Type

    Empty backbone

    Use

    Zebrafish enhancer assay vector

    Promoter

    E1b

    Available Since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-mGreenLantern

    Plasmid

    #161912

    Purpose

    mGreenLantern fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHM6-alphasynuclein-WT

    Plasmid

    #40824

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Tags

    6x His and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. VAMP2-pHuji

    Plasmid

    #105289

    Purpose

    VAMP2 tagged with pH-sensitive red fluorescent protein pHuji

    Depositor

    Justin Taraska

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAMP2 (Vamp2 Rat)
    

    Tags

    pHuji

    Expression

    Mammalian

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSFFV_sfCherry2_TagBFP

    Plasmid

    #83031

    Purpose

    Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry+full length_TagBFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    3 mutations in sfCherry full length: E118Q, T128I…

    Promoter

    SFFV

    Available Since

    Jan. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. TetO-FUW-NfiB

    Plasmid

    #64900

    Purpose

    Inducible expression of murine CDS encoding for NfiB. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiA allows reprograming into iAstro. cells.

    Depositor

    Vania Broccoli

    Article

    Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

    Insert

    NfiB (Nfib Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    July 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCh-Drp1

    Plasmid

    #49152

    Purpose

    mammalian expression of Drp1 fused to mCherry

    Depositor

    Gia Voeltz

    Article

    Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

    Insert

    Drp1 (DNM1L Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBMN-YFP-Parkin

    Plasmid

    #59416

    Purpose

    to make YFP-Parkin stable cell line with retrovirus

    Depositor

    Richard Youle

    Article

    Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

    Insert

    YFP-Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-AKAR4

    Plasmid

    #61619

    Purpose

    FRET-based biosensor for monitoring PKA activity

    Depositor

    Jin Zhang

    Article

    Depry et al Mol Biosyst. 2011 Jan;7(1):52-8. doi: 10

    Insert

    AKAR4
    

    Tags

    Cerulean and cpVenus[E172]

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-GFP-MDC1 (770-7)

    Plasmid

    #26284

    Depositor

    Eric Campeau

    Insert

    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Entry vector

    Tags

    GFP

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHes1(2.5k)-luc

    Plasmid

    #43806

    Depositor

    Ryoichiro Kageyama

    Article

    Takebayashi et al J Biol Chem. 1994 Feb 18;269(7):5150-6.

    Insert

    Hes1 Promoter (Hes1 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains the murine Hes1 promoter

    Promoter

    Hes1 Promoter

    Available Since

    March 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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