We narrowed to 9,224 results for: Coli

1. PT7lacO-RRF-His6

   Plasmid

   #224374

   Purpose

   Express RRF protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   RRF (frr E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PT7lacO-His6-IF3

   Plasmid

   #224368

   Purpose

   Express IF3 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   IF3 (infC E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PT7lacO-His6-IF1

   Plasmid

   #224366

   Purpose

   Express IF1 protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   IF1 (infA E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PT7lacO-His6-ThrRS

   Plasmid

   #224365

   Purpose

   Express ThrRS protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   ThrRS (thrS E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PT7lacO-His6-NDK

   Plasmid

   #224376

   Purpose

   Express NDK protein for his-tagged purification

   Depositor

   Richard Murray

   Article

   Zhang et al bioRxiv 2024.06.19.599772

   Insert

   NDK (ndk E. coli)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAC5.1-bgal-V5His

   Plasmid

   #136238

   Purpose

   Protein expression of Beta-galactosidase with C-terminal V5 and His tags

   Depositor

   Steven Reppert, Aziz Sancar

   Article

   Zhu et al Curr Biol. 2005 Dec 6;15(23):R953-4. doi

   Insert

   Beta-galactosidase
   

   Use

   Tags

   His and V5

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPR220.08

   Plasmid

   #78553

   Purpose

   Plasmid constitutively expressing phycocyanobilin (PCB) biosynthesis genes and UirS sensor kinase gene

   Depositor

   Jeffrey Tabor

   Article

   Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

   Insert

   uirS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   J23108

   Available since

   June 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSB1A3-AD011

   Plasmid

   #116852

   Purpose

   Expresses araC constitutively and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD011

   Depositor

   Friedrich Simmel

   Article

   Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

   Inserts

   AraC
   mScarletI
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBbS5c-mCherry-AsLOV2*(543)

   Plasmid

   #202712

   Purpose

   Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

   Depositor

   Mary Dunlop

   Article

   Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

   Insert

   mCherry-AsLOV2*(543)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAN1717

    Plasmid

    #74696

    Purpose

    RPU standard plasmid

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    Insulated J23101-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJLG039

    Plasmid

    #192982

    Purpose

    BHR plasmid (IncP ori) expressing T7 polymerase under control of the lac promoter

    Depositor

    Clay Fuqua

    Article

    Greenwich et al Microbiol Resour Announc. 2023 Feb 16;12

    Insert

    T7 polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPL5071_TEF1*-Cre_URA3

    Plasmid

    #60930

    Purpose

    Constitutive expression vector for Cre-recombinase containing URA3 selection marker.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Cre
    

    Use

    Tags

    Driven from a mutant TEF1 promoter with 0.16x act…

    Expression

    Yeast

    Mutation

    Promoter

    mutant TEF1

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDisplay-AP-CFP-TM

    Plasmid

    #20861

    Depositor

    Alice Ting

    Article

    Howarth et al Proc Natl Acad Sci U S A. 2005 May 24. 1

    Insert

    AP-CFP-TM
    

    Use

    Tags

    CFP, HA, and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pC28-LIC-sfGFP-His

    Plasmid

    #165479

    Purpose

    pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    TEV-GFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTW357bN

    Plasmid

    #227932

    Purpose

    Accessory plasmid for negative selection

    Depositor

    Tina Wang

    Article

    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

    Inserts

    gIII-neg
    T7c
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R632S

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTW357d5

    Plasmid

    #227931

    Purpose

    High-stringency accessory plasmid for selection

    Depositor

    Tina Wang

    Article

    Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

    Inserts

    gIII (recoded)
    T7c
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R632S, Q649S

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDR-GW FLII12Pglu-700μδ6

    Plasmid

    #28002

    Depositor

    Wolf Frommer

    Article

    Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

    Insert

    FLII12Pglu-700μδ6
    

    Use

    Tags

    eCFP and eYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 7, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pPB-R1R2-EM7neoPheS

    Plasmid

    #112923

    Purpose

    piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    neoPheS
    

    Use

    Piggybac transposon gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRF-BetI

    Plasmid

    #49357

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    BetI repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAH69

    Plasmid

    #173782

    Purpose

    The pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.

    Depositor

    Keith Shearwin

    Article

    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

    Insert

    HK022 Integrase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMA3735

    Plasmid

    #184848

    Purpose

    gRNA cloning using blue-white screening for recombinants

    Depositor

    Mikhail Alexeyev

    Article

    Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

    Insert

    LacZalpha
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    alpha-fragment of LacZ

    Promoter

    U6

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBEST-OR2-OR1-Pr-UTR1-deGFP-T500

    Plasmid

    #40019

    Depositor

    Vincent Noireaux

    Article

    Shin et al J Biol Eng. 2010 Jun 24;4:8.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

    Available since

    Oct. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKB231

    Plasmid

    #170018

    Purpose

    Encodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Cecropin P1
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21-Streptavidin-Glutamate_Tag

    Plasmid

    #46367

    Depositor

    Mark Howarth

    Article

    Fairhead et al J Mol Biol. 2013 Sep 19. pii: S0022-2836

    Insert

    Streptavidin-Glutamate_Tag
    

    Use

    Tags

    6 glutamate tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pKL09-GCaMP6f-mScarlet bb118

    Plasmid

    #158979

    Purpose

    Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    GCaMP6f-mScarlet fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#100833

    Promoter

    Bba_J23118 (sigma 70)

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAT112TAG-SepT

    Plasmid

    #34624

    Depositor

    Dieter Söll, Jesse Rinehart

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    tRNA^Sep
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMYO

    Plasmid

    #34626

    Depositor

    Dieter Söll, Jesse Rinehart

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    Myoglobin
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    lpp

    Available since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pADS

    Plasmid

    #19040

    Depositor

    Jay Keasling

    Article

    Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

    Insert

    codon-optimized synthetic amorphadiene synthase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

29. OmpX

    Plasmid

    #213136

    Purpose

    Expresses outer membrane protein X

    Depositor

    Sebastian Hiller

    Article

    Rath et al Biochim Biophys Acta Biomembr. 2020 Jan

    Insert

    OmpX (24-171) (ompX E.coli (K12))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMA2641

    Plasmid

    #25435

    Depositor

    Mikhail Alexeyev

    Article

    Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

    Insert

    reverse tetracycline-regulated transactivator advanced
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge