We narrowed to 30,130 results for: REP

1. Ac5-STABLE2-blast

   Plasmid

   #208034

   Purpose

   Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter.

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-ymNeongreen-URA3

   Plasmid

   #168059

   Purpose

   Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeonGreen
   

   Tags

   ymNeonGreen

   Expression

   Yeast

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Tet-on circRNA Expression Vector

   Plasmid

   #92351

   Purpose

   The vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.

   Depositor

   Ling-Ling Chen

   Article

   Zhang et al Cell Rep. 2016 Apr 19;15(3):611-24. doi:

   Insert

   mCherry and split EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   pTet

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. p5154

   Plasmid

   #175390

   Purpose

   High Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)

   Depositor

   Michael Wiles

   Article

   Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting; Cloning vector, to be used to de…

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmCherry-T2A-moxMaple3

   Plasmid

   #120875

   Purpose

   Mammalian expression of mCherry and moxMaple3

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

   Inserts

   mCherry
   moxMaple3
   

   Expression

   Mammalian

   Mutation

   from mMaple3, moxMaple3 was engineered by replaci…

   Available Since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. sReach-mTurquoise2

   Plasmid

   #98840

   Purpose

   Produces a fusion between mTurquoise2 and sReach. It can be used as a positive control for FRET (using FLIM)

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   sReach-mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDB179-HotPETase

   Plasmid

   #218701

   Purpose

   Encodes codon optimized HotPETase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   HotPETase
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5155

   Plasmid

   #175391

   Purpose

   Low Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts >15kb) into dual site mouse strains (Bxb-GT/GA)

   Depositor

   Michael Wiles

   Article

   Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting; Cloning vector, to be used to de…

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Rogi1-CMV-LAMB3-T2A-GFP-bGH

   Plasmid

   #179864

   Purpose

   Targets Rogi1 genomic region with HDR and expresses LAMB3-T2A-GFP

   Depositor

   George Church

   Article

   Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

   Insert

   LAMB3-T2A-GFP
   

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-Renilla

    Plasmid

    #79496

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Renilla Luciferase CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    Renilla Luciferase
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-EGxxFP

    Plasmid

    #50716

    Purpose

    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    split EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPRv2 sgSp1

    Plasmid

    #174907

    Purpose

    Second generation sgRNA against Sp1

    Depositor

    Jane Clifford

    Article

    Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

    Insert

    Specificity Protein 1 (SP1 Human)
    

    Expression

    Mammalian

    Mutation

    Missense mutation to mutate PAM sequence

    Promoter

    EFS-NS

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

    Plasmid

    #188703

    Purpose

    Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

    Depositor

    David Tobin

    Article

    Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AIO-mCherry

    Plasmid

    #74120

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDB179-DuraPETase

    Plasmid

    #218699

    Purpose

    Encodes codon optimized DuraPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    DuraPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmcherry-LCa

    Plasmid

    #53972

    Purpose

    Expresses the light chain of clathrin

    Depositor

    Tom Kirchhausen

    Article

    Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

    Insert

    clathrin light chain
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pBbS8k-RFP_R43W_E93G

    Plasmid

    #109406

    Purpose

    RepA Mutant

    Depositor

    Jay Keasling

    Article

    Thompson et al Sci Rep. 2018 Jan 25;8(1):1590. doi: 10.

    Insert

    repA
    

    Tags

    RFP

    Expression

    Bacterial

    Mutation

    R43W and E93G repA mutations to increase copy num…

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLSSmOrange-mKate2

    Plasmid

    #99868

    Purpose

    Measure FRET between LSSmOrange and mKate2

    Depositor

    Marc Tramier

    Article

    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

    Insert

    LSSmOrange
    

    Tags

    mKate2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-AAV9cap

    Plasmid

    #198016

    Purpose

    For high-level expression of the AAV9 cap gene, suitable for use with pCMV-Rep78/68 SSM library

    Depositor

    George Church

    Article

    Jain et al Elife. 2024 Mar 27;12:RP87730. doi: 10.7

    Insert

    AAV9 cap
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. p.UTA.3.0 Empty

    Plasmid

    #82447

    Purpose

    [Edit] Dual Fluorescent endogenous miRNA sensor. To insert the complementary region of the 3'UTR of RFP.

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Type

    Empty backbone

    Use

    RNAi

    Expression

    Mammalian

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pNCS-mClover3

    Plasmid

    #74236

    Purpose

    Constitutive expression of mClover3 in bacteria

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Has Service

    Cloning Grade DNA

    Insert

    mClover3
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCAG-Apex2-Venus-CAAX

    Plasmid

    #168511

    Purpose

    Expression of Apex2 fused to Venus-CAAX for targeting to plasma membrane. Useful for correlated light-eletron microscopy experiments in neurons.

    Depositor

    Franck Polleux

    Article

    Hirabayashi et al Sci Rep. 2018 Sep 27;8(1):14491. doi: 10

    Insert

    Apex2
    

    Tags

    Venus-CAAX

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pRSETA-qmTQ2-cAMP

    Plasmid

    #225249

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-cAMP
    

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. Influenza-FPV-HA-mEGFP

    Plasmid

    #127810

    Purpose

    Expression vector Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion protein, plasma membrane localization

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

    Insert

    Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion
    

    Expression

    Mammalian

    Available Since

    June 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTXTL-T7max-RLuc

    Plasmid

    #188312

    Purpose

    Expression of Renilla luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Renilla luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Promoter

    T7Max

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDB179-TurboPETase

    Plasmid

    #218693

    Purpose

    Encodes codon optimized TurboPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    TurboPETase
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNCS-mRuby3

    Plasmid

    #74234

    Purpose

    Constitutive expression of mRuby3 in bacteria

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Insert

    mRuby3
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTXTL-T7max-FLuc

    Plasmid

    #188311

    Purpose

    Expression of Firefly luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Firefly luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Promoter

    T7Max

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pCAT001

    Plasmid

    #171061

    Purpose

    Plasmid for expressing HIV-1 envelope glycoprotein

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    HIV-1 Env
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mNeonGreen-2A-mTurquoise2

    Plasmid

    #98885

    Purpose

    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mNeonGreen-2A-mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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