We narrowed to 19,131 results for: Tat

Showing: 1641 - 1660 of 19131 results

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Spectrin-C-cpstFRET

Plasmid

#61111

Purpose

Expression of Spectrin-C-term cpstFRET to measure mechanical stress and report multidimensional stress fields

Depositor

Fred Sachs

Article

Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

Insert

spectrin-cpstFRET

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pRH2502

Plasmid

#84379

Purpose

Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

dcas9

Use

CRISPR

Expression

Bacterial

Mutation

Aspartate at position 10 changed to Alanine, Hist…

Promoter

uv15tetO

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE2_EF1a_TetOn_IRES_mCherry

Plasmid

#204155

Purpose

Lentivirus backbone expressing rtTA linked to mCherry

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Insert

Tet-On 3G

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-sREACh-Actin

Plasmid

#21949

Depositor

Ryohei Yasuda

Article

Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

Insert

betaActin

Expression

Mammalian

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only
YF-Rac1(CA)

Plasmid

#20150

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Rac1(CA) (RAC1 Human)

Expression

Mammalian

Mutation

constitutively active mutation

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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LPCX BMP-RIB Q203D

Plasmid

#12642

Depositor

Rik Derynck

Article

Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

Insert

BMP-RIB (Bmpr1b Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Q203D, activating mutation

Available Since

Oct. 17, 2006

Availability

Academic Institutions and Nonprofits only
pHSN501

Plasmid

#50589

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

zCas9D10A
gRNA scaffold

Use

CRISPR; Plant expression

Tags

3xFLAG and NLS

Mutation

Cas9D10A nickase, was derived from the zCas9 and …

Promoter

2×35Sp and AtU6-26p

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST14-SpyCatcher EQ

Plasmid

#35045

Depositor

Mark Howarth

Article

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyCatcher EQ

Tags

His6

Expression

Bacterial

Mutation

Deletion of C-terminal till I113, and point mutat…

Available Since

April 18, 2012

Availability

Academic Institutions and Nonprofits only
pDuet TDP-43 M337V

Plasmid

#27464

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Expression

Bacterial

Mutation

Methionine 337 mutated to Valine (M337V)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pORANGE GFP-Actb KI #2

Plasmid

#139666

Purpose

Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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TRIPZ-bioGEF-UBnc-PURO

Plasmid

#208044

Purpose

Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

UBnc (UBC Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Mutation

L73P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

Plasmid

#128424

Purpose

Human Codon Optimized dCas9-DNMT3A3L

Depositor

Bradley Bernstein

Article

Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

Insert

dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP

Use

CRISPR and Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

T7 Promoter

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAR-Ec318

Plasmid

#130871

Purpose

A nuclear plasmid encoding the WT TP-DNAP1 for OrthoRep

Depositor

Chang Liu

Article

Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

Insert

TP-DNAP1

Expression

Yeast

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP-HA-hM3D(Gq)-IRES-mCitrine

Plasmid

#50470

Purpose

Gq-coupled hM3D-IRES-mCitrine under the control of GFAP promoter

Depositor

Bryan Roth

Insert

hM3D(Gq)-IRES-mCitrine (CHRM3 Human)

Use

AAV

Tags

HA

Mutation

See supplemental documents for DREADD mutations

Promoter

GFAP

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223_MYC_WT

Plasmid

#82927

Purpose

Gateway Donor vector containing MYC, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

Myc (MYC Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST-V2-ORF

Plasmid

#73636

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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pNMT

Plasmid

#42578

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

N-myristoyl tranferase (NMT1 Budding Yeast)

Expression

Bacterial

Mutation

two silent mutations T690C and T942C

Promoter

T7

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
EF.deltaBHES1.Ubc.GFP

Plasmid

#24982

Depositor

Linzhao Cheng

Article

Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

Insert

HES1 (HES1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

DNA-binding domain disrupted by E43A, K44A, R47A …

Available Since

June 14, 2010

Availability

Academic Institutions and Nonprofits only
pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

Plasmid

#183409

Purpose

piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1

Use

CRISPR and Synthetic Biology; Piggybac

Expression

Mammalian

Promoter

TRE3G

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-YFP-miniTurbo-L2

Plasmid

#127352

Purpose

gateway entry vector for making N-terminal YFP-miniTurbo fusions with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

miniTurbo (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, V5, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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