We narrowed to 7,177 results for: tet-on

1. pBEST-p15a-pBAD-UTR1-TetR-T500

   Plasmid

   #45787

   Depositor

   Richard Murray

   Insert

   Tetracycline repressor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai

   Plasmid

   #202571

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

   Plasmid

   #199346

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHR-CMV-TetO2_3C-mVenus-Twin-Strep

   Plasmid

   #113891

   Purpose

   Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

   Depositor

   A. Radu Aricescu

   Article

   Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-mVenus-Twin-Strep

   Expression

   Mutation

   Promoter

   CMV-MIE-TetO2

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLQ-Pxyl/tet-cas9-Pj23119-sgRNA

   Plasmid

   #92121

   Purpose

   CRISPR-Cas9 based efficient genome editing in S. aureus

   Depositor

   Sheng Yang

   Article

   Liu et al Acta Biochim Biophys Sin (Shanghai). 201

   Insert

   Cas9-Pxyltet-sgRNA-pj23119
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBBR1-kan-Ptet-plu-gamma-ET_rhi145

   Plasmid

   #180427

   Purpose

   Genome engineering of Agrobacterium

   Depositor

   Jun Fu

   Article

   Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

   Insert

   plu-gamma-ET_rhi145
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TetO7-HSP30UTR-LacZ-PKTlinker-nLucPEST-MS2(v4)

   Plasmid

   #199763

   Purpose

   Elongation reporter construct to quantify elongation of LacZ

   Depositor

   Brian Zid

   Article

   Guzikowski et al RNA Biol. 2022;19(1):636-649. doi: 10.10

   Insert

   LacZ-nLucPEST
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TetO7

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

   Plasmid

   #121185

   Purpose

   OsTIR1 introduction

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Tet-OsTIR1-loxP-PURO-loxP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   OsTIR1 codon optimized for human cells

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3

   Plasmid

   #200631

   Purpose

   Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing pad

   Depositor

   Kenneth Matreyek

   Article

   Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

   Insert

   iCasp9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   pTRE3G

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid

    #100596

    Purpose

    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Inserts

    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for human codon usage

    Promoter

    Dox-inducible TetO promoter and Human EEF1A1 prom…

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPB-tetpA(hBCL2-miR9/9*-124)-iRPT

    Plasmid

    #154881

    Purpose

    Doxycycline-dependently expresses human BCL2 and murine miR9/9*-124. Ubiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.

    Depositor

    Hideyuki Okano

    Article

    Nemoto et al Cells. 2020 Dec 22;10(1). pii: cells1001

    Inserts

    iRFP713
    Puromycin resistance gene
    rtTAM2
    BCL2 (BCL2 Human)
    miR9/9*-124
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAV-H1promoter-2xTetO-Tornado-1xALFA-5xSunTag

    Plasmid

    #231597

    Purpose

    Express circular RNA translation reporter with 1xALFATag-5xSunTag under H1 promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-1xALFATag-5xSunTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

    Plasmid

    #199356

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD

    Plasmid

    #196086

    Purpose

    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting lncRNA NORAD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBBR1-kan-Ptet-plu-gamma-ETh_rhi483

    Plasmid

    #180429

    Purpose

    Genome engineering of Agrobacterium

    Depositor

    Jun Fu

    Article

    Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

    Insert

    plu-gamma-ETh_rhi483
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCW-Tet-Cas9-miRFP670nano-PGK-Blast

    Plasmid

    #196722

    Purpose

    Tet-inducible expression of Cas9-T2A-miRFP670nano

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-miRFP670nano
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG-SV40NLS and Nucleoplasmin NLS

    Expression

    Mutation

    Promoter

    TRE, PGK

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

    Plasmid

    #113890

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRRL-5xTetO-pEF-H2B-Citrine-SV40

    Plasmid

    #186966

    Purpose

    Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

    Depositor

    Brian Liau

    Article

    Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

    Insert

    5xTetO-pEF-H2B-Citrine-SV40
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5xTetO-pEF-1alpha

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GB1-His-TET1-848-952(pL91)

    Plasmid

    #124388

    Purpose

    E.Coli produced TET1-848-952 purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-TET1-848-952 wildtype (TET1 Human)
    

    Use

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEST-p15A-Pl-tetO1-deGFP-T500 (122p)

    Plasmid

    #70198

    Purpose

    Promoter panel

    Depositor

    Richard Murray

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    deGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge