12,610 results

1. pFUW-TetO-Junb

   Plasmid

   #178441

   Purpose

   doxycycline-inducible expression of Mouse Junb in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

   Insert

   Junb (Junb Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBABE-puro-mTwist

   Plasmid

   #1783

   Depositor

   Bob Weinberg

   Article

   Yang et al Cell 2004 Jun 25;117(7):927-39.

   Insert

   Twist (Twist1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBac HT JS-Munc18c

   Plasmid

   #135555

   Purpose

   Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   Munc18c
   

   Use

   Tags

   6xHis tag and TEV site

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFRT-PonA-BI-Gl-WT-24xMS2-WT-18xPP7 (WW)

   Plasmid

   #189791

   Purpose

   Express wild-type beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

   Depositor

   Robert Singer

   Article

   Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

   Insert

   beta-globin (HBB Mouse, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ponasterone A inducible

   Available since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mTagBFP2-MannII-N-10

   Plasmid

   #55309

   Purpose

   Localization: Mannosidase/Golgi, Excitation: 399, Emission: 456

   Depositor

   Michael Davidson

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   MannII
   

   Use

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   aa 1-112 of MannII

   Promoter

   CMV

   Available since

   Oct. 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA-Flag-Kdm4b-pA

   Plasmid

   #104486

   Purpose

   Express mouse Kdm4b in mammalian cells. It has a T7 promoter for in vitro transcription. Since polyA is encoded on the vector, polyA-tailing is unnecessary.

   Depositor

   Yi Zhang

   Article

   Inoue et al Genes Dev. 2017 Oct 1;31(19):1927-1932.

   Insert

   Kdm4b (Kdm4b Mouse)
   

   Use

   Tags

   Flag epitope tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCLXEBR-pTF-cKit

   Plasmid

   #114293

   Purpose

   TET-inducible expression of c-Kit

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   cKit (Kit Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SNX9-pmCherryC1

   Plasmid

   #27678

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   SNX9 (Snx9 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. aMHC-mCherry-Rex-Blasticidin

   Plasmid

   #21228

   Depositor

   Mark Mercola

   Article

   Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

   Insert

   alpha myosin heavy chain promoter
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid

    #159908

    Purpose

    Mutagenesis of Htr2a

    Depositor

    Larry Zweifel

    Insert

    Htr2a (Htr2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO-Tet-On-shRab27a

    Plasmid

    #120930

    Purpose

    conditionally knockdown Rab27a in mouse cell lines

    Depositor

    Mien-Chie Hung

    Article

    Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

    Insert

    shRab27a (Rab27a Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PCDNA3 Flag p38 alpha (agf)

    Plasmid

    #20352

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Replace Thr 180 and Tyr 182 with Ala and Phe resp…

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR iSH-YFP-PIF

    Plasmid

    #50841

    Purpose

    Optogenetic PI3K activation system; cytoplasmic component

    Depositor

    Wendell Lim

    Article

    Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

    Inserts

    p85 iSH2
    PIF6 1-100
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEF1a-mRor2WT

    Plasmid

    #22613

    Depositor

    Roel Nusse

    Article

    Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

    Insert

    Ror2 WT (Ror2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-Akt DN (K179M)

    Plasmid

    #16243

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al J Biol Chem. 2000 Mar 17. 275(11):8027-3

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Dominant negative (K179M)

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-FLAG-Bbs4

    Plasmid

    #128868

    Purpose

    For mammalian expression of mouse Bbs4

    Depositor

    Aimin Liu

    Article

    Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

    Insert

    Bbs4 (Bbs4 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMIH-mRuby2-BAX

    Plasmid

    #111628

    Purpose

    Fluorescent fusion protein used to visualise mouse BAX, with hygromycin selection

    Depositor

    Benjamin Kile

    Article

    McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

    Insert

    Bax (Bax Mouse)
    

    Use

    Retroviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Stat3 Y705F Flag pRc/CMV

    Plasmid

    #8709

    Depositor

    Jim Darnell

    Article

    Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

    Insert

    Stat3 Y705F (Stat3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y705F

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-TET-MKOS

    Plasmid

    #20959

    Purpose

    piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    cMyc, KLF4, Oct4, Sox2 (Myc, Klf4, Pou5f1, Sox2 Mouse)
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

    Plasmid

    #67975

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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