We narrowed to 7,580 results for: Ski;

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pPU-Empty-miR30-TRIM5

Plasmid

#132936

Purpose

All-in-one control rescue-TRIM5 shRNA knockdown vector

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

control shRNA

Use

Lentiviral and RNAi

Tags

HA

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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GAL 103Q+ProCFPp303

Plasmid

#15575

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt103Q + Pro (HTT Human)

Tags

CFP

Expression

Yeast

Mutation

Proline-rich region present

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
MAC-SARS-CoV-2 NSP8

Plasmid

#158359

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP8 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 NSP10

Plasmid

#158361

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP10 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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HOXC8-pSG5

Plasmid

#21001

Depositor

Corey Largman

Article

Lawrence et al Cell Growth Differ. 1993 Aug . 4(8):665-

Insert

HOXC8 (HOXC8 Human)

Expression

Mammalian

Mutation

missing first 24 N-terminal amino acids of coding…

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pNAS1b split mCherry 14-3-3σ

Plasmid

#111881

Purpose

Mode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Tags

Split mCherry system: 14-3-3σ fused to C-terminal…

Expression

Bacterial

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-GPR78

Plasmid

#158382

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GPR78 (GPR78 Human)

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX Aps-SH2

Plasmid

#46404

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Aps (SH2B2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 404-523

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-flex-rev-g2-2A-Venus

Plasmid

#71410

Purpose

This plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.

Depositor

William Wisden

Article

Uygun et al J Neurosci. 2016 Nov 2;36(44):11171-1118

Inserts

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

mouse hdc gene promoter, works as a pan neuronal …

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAsCpf1GG

Plasmid

#131011

Purpose

Backbone plasmid for generating CRISPR arrays for AsCas12a. Contains a GFP-dropout cassette and a direct repeat.

Depositor

Chase Beisel

Article

Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

Inserts

direct repeat of AsCas12a
GFP expression cassette

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pUASP dsRed Rab11 CA

Plasmid

#44830

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

Q70L (constitutive active)

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pLenti CRISPR V2 sgMTFMT_1

Plasmid

#106318

Purpose

Express Cas9 and sgRNA targeting MTFMT

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTFMT

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-GW-V5-Hygro

Plasmid

#68265

Purpose

Constitutive selectable expression of C-term V5 tagged proteins

Depositor

Mitchell Guttman

Article

McHugh et al Nature. 2015 May 14;521(7551):232-6. doi

Type

Empty backbone

Tags

V5

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only
pEY68

Plasmid

#191065

Purpose

ceh-14 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ceh-14 (ceh-14 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX Syk(C)-SH2

Plasmid

#46519

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Syk(C) (SYK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 149-263

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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GAL FLAG46QDProCFPp303

Plasmid

#15585

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pET51b-Strep-SsOGT-H5-His

Plasmid

#167277

Purpose

Overexpression of a sulfolobal equivalent of SNAP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

SsOGT-H5

Tags

His x10 and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

Plasmid

#91873

Purpose

Photo-activatable/light sensitive Vav2

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
VAV2 DH/PH/ZF (Vav2 Mouse)

Tags

mCherry

Expression

Bacterial and Mammalian

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX Stat4-SH2

Plasmid

#46514

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat4 (STAT4 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 133-678

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA pDEST40 Cas9 GW STOP

Plasmid

#183196

Purpose

Destination vector

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
GPD 25QDProGFP p416

Plasmid

#15568

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q Delta Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pEY64

Plasmid

#191061

Purpose

aqp-6 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

aqp-6 (aqp-6 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX Slap-SH2

Plasmid

#46500

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slap (SLA Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 61-221

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZLblasti-CTR1M154A

Plasmid

#53158

Purpose

Expresses human CTR1 M154A from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

CTR1 (SLC31A1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

M154A

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pGEX Vav1-SH2

Plasmid

#46528

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Vav1 (VAV1 Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 661-777

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pUASp dsRed Rab5 CA

Plasmid

#53467

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab05 (Rab5 Fly)

Tags

dsRed

Expression

Insect

Mutation

Q88L

Promoter

GAL4

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
GPD 104QDProGFP p416

Plasmid

#15569

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 104Q Delta Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_YFP

Plasmid

#45278

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

YFP

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
GAL FLAG72QDProCFPp303

Plasmid

#15586

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pPN022

Plasmid

#91668

Purpose

Express sgRNA targeting human SHANK3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SHANK3 (SHANK3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
SIN40C.SFFV.dTomato.miR-ctrl

Plasmid

#169303

Purpose

Constitutive overexpression of miR-ctrl with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-ctrl

Use

Lentiviral

Tags

dTomato

Promoter

SFFV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Cas9-HMGB1

Plasmid

#183198

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-HMGB1

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-SERTS585P-myc

Plasmid

#107498

Purpose

Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotype

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Serotonin Transporter (SERT) (SLC6A4 Human)

Expression

Mammalian

Mutation

S585P

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221 Cas9-RFC4

Plasmid

#183199

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-RFC4

Use

CRISPR; Gateway entry clone

Mutation

n/a

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe_puro_DEST_Flag_SAMD7

Plasmid

#45275

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

SAMD7 (SAMD7 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pGEX Eat2-SH2

Plasmid

#46423

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Eat2 (SH2D1B Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-132

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASP dsRed Rab5 DN

Plasmid

#44823

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab5 (Rab5 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

S43N (dominant negative)

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pMS1088

Plasmid

#80159

Purpose

pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

Depositor

Marcelo Sousa

Article

Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

Insert

HisSUMO-Cys-4xNuG2-Ald

Tags

HisSUMO

Promoter

T7

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX PLCg1(N)-SH2

Plasmid

#46470

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(N)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 539-664

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti CRISPR sgSHMT1_2

Plasmid

#106312

Purpose

Express Cas9 and sgRNA targeting SHMT1

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting SHMT1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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