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Addgene

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159,134 results

Showing: 1741 - 1760 of 159134 results

  2. Lamp1-mNeonGreen

    Plasmid
    #98882
    Purpose
    In vivo visualization of lysosomes (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    Lamp1
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    98882-plasmid-map-sequence-id-191165

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  6. InterTag-3xL

    Plasmid
    #186913
    Purpose
    Sleeping Beauty transposon system with constitutive bi-directional promoter, one side expressing SpyTag003-sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side expressing BFP and puromycin resistance gene
    Depositor
    Taekjip Ha, Allen Liu
    Article
    Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100
    Insert
    SpyTag003-sfCherry11-(GGGGS)3-PDGFR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    186913-plasmid-map-sequence-id-369408

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  7. pSDMA57

    Plasmid
    #67941
    Purpose
    Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains NEO resistance marker.
    Depositor
    James Fraser
    Article
    Arras et al PLoS One. 2015 Apr 9;10(4):e0122916. doi
    Type
    Empty backbone
    Use
    Cryptococcal complementation vector
    Tags
    Expression
    Mutation
    Promoter
    ACT1 for Cryptococcal marker
    Available since
    Oct. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    67941-plasmid-map-sequence-id-120526

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  13. pLenti PGK Puro GFP-LC3B G120

    Plasmid
    #123241
    Purpose
    Expression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.
    Depositor
    Robin Ketteler
    Article
    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155
    Insert
    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deleted amino acids 121-125. Stop codon after G12…
    Promoter
    PGK
    Available since
    May 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    123241-plasmid-map-sequence-id-245043

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  14. TFORF0846

    Plasmid
    #141732
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    YBX1 (YBX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141732-plasmid-map-sequence-id-404379

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  15. pCSC-LHX8-IRES-GBX1

    Plasmid
    #182235
    Purpose
    To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with ASCL1, Sox11, FGF2 and two small molecules, forskolin and LDN-193189
    Depositor
    Chun-Li Zhang
    Article
    Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.
    Insert
    LHX8-IRES-GBX1 (LHX8, GBX1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    CMV
    Available since
    March 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    182235-plasmid-map-sequence-id-357464

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  16. qTAG-N-Puro-mNeon-ACTB

    Plasmid
    #207752
    Purpose
    Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    207752-plasmid-map-sequence-id-414897

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  17. pET-29b(+)_His-CBD-SpEndoH

    Plasmid
    #218196
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH
    Depositor
    Florence Pojer
    Insert
    EndoH
    Use
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-313
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218196-plasmid-map-sequence-id-431799

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Showing: 1741 - 1760 of 159134 results