We narrowed to 5,867 results for: nls

1. pSL1315

   Plasmid

   #129527

   Purpose

   pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   dSpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   NLS, 1xHA

   Expression

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p35SZ

   Plasmid

   #51513

   Purpose

   pMDC32 with Zif268:FokI followed by approximately 3kb of filler sequence

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   Zif268:FokI
   3kb filler sequence
   

   Use

   Plant expression t-dna

   Tags

   NLS

   Expression

   Mutation

   Promoter

   2x35S

   Available since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLSLZ.D

   Plasmid

   #51496

   Purpose

   pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   Zif268:FokI
   us:NPTII repair template
   

   Use

   Plant t-dna plasmid

   Tags

   SV40 NLS

   Expression

   Mutation

   Promoter

   virion-sense LIR and 2x35S

   Available since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIVT-Bxb1

   Plasmid

   #232746

   Purpose

   IVT template for making modRNA encoding Bxb1

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Bxb1
   

   Use

   In vitro transcription

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pdCas9-GFP-p65

   Plasmid

   #232630

   Purpose

   p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer

   Depositor

   Karsten Rippe

   Insert

   dCas9-GFP-p65 (RELA Synthetic, Human)
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PX458_3XHA_dFnCas9_KRAB_T2A_eGFP

   Plasmid

   #200389

   Purpose

   3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

   Depositor

   Debojyoti Chakraborty

   Insert

   PX458_3XHA_dFnCas9_KRAB_eGFP
   

   Use

   CRISPR

   Tags

   3xHA-NLS and SV40NLS-KRAB-T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CBH

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS42K_MCP-VP64_D-MS2

   Plasmid

   #177161

   Purpose

   Expresses transactivator (MCP-VP64) and scRNA (D-MS2) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   MCP-VP64
   D-MS2
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter and SNR52 promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS42K_PCP-VP64_A-PP7

   Plasmid

   #177156

   Purpose

   Expresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   PCP-VP64
   A-PP7
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter and SNR52 promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS42K_MCP-VP64_C-MS2

   Plasmid

   #177160

   Purpose

   Expresses transactivator (MCP-VP64) and scRNA (C-MS2) in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Inserts

   MCP-VP64
   C-MS2
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter and SNR52 promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EC0_4_VPR

    Plasmid

    #163716

    Purpose

    Plasmid encoding VPR (VP64 - SV40 NLS - p65 - Rta) as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    VPR
    

    Use

    Synthetic Biology; Moclo vector level 0 (type 4a)

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TC512

    Plasmid

    #164888

    Purpose

    Pmig-13::Zip(-)::QFAD::NLS::SL2::mCherry

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    zip-QFAD
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSL1211

    Plasmid

    #129521

    Purpose

    pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    SpCas9
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    3XFlag-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEPOR0SP0008

    Plasmid

    #117535

    Purpose

    Level0 Golden Gate part: CDS, AsCas12a

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    AsCas12a (with stop codon)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDS2

    Plasmid

    #70135

    Purpose

    CEN/ARS yeast shuttle vector with DS2 selection marker

    Depositor

    Morten Sommer

    Article

    Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

    Insert

    DS2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    AgTEF1

    Available since

    Dec. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL4.23-IL2RA CaRE4 (plus strand)

    Plasmid

    #91850

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0345)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL4.23-IL2RA CaRE4 scramble (plus strand)

    Plasmid

    #91852

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0347)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 scramble (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Sequence scrambled from ch10:6094588-6094934

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4.23-IL2RA CaRE4 (minus strand)

    Plasmid

    #91849

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0626)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL4.23-IL2RA CaRE1 (plus strand)

    Plasmid

    #91837

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0614)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE1 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL4.23-CD69 CaRE3 (plus strand)

    Plasmid

    #91844

    Purpose

    Luciferase reporter for CD69 enhancer (IGI-P0621)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    CD69 CaRE3 (plus strand) (CD69 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Missing 1 base from 13 base polyT tract beginning…

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL4.23-IL2RA CaRE5 (plus strand)

    Plasmid

    #91841

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0618)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE5 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL4.23-IL2RA CaRE3 (minus strand)

    Plasmid

    #91835

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0612)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE3 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    rs7893324 A>G, rs7090530 G>T, rs7090512 A&g…

    Promoter

    Available since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGL4.23-CD69 CaRE3 (minus strand)

    Plasmid

    #91845

    Purpose

    Luciferase reporter for CD69 enhancer (IGI-P0622)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    CD69 CaRE3 (minus strand) (CD69 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Missing 1 base from 13 base polyT tract beginning…

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGL4.23-IL2RA CaRE4 SNP (plus strand)

    Plasmid

    #91851

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0346)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE4 SNP (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    rs61839660 C>T

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL4.23-IL2RA CaRE5 (minus strand)

    Plasmid

    #91836

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0613)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE5 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL4.23-IL2RA CaRE2 (plus strand)

    Plasmid

    #91846

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0623)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE2 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    rs6602425 T>G, T>C at chr10:6117603

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGL4.23-IL2RA CaRE2 (minus strand)

    Plasmid

    #91847

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0624)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE2 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    rs6602425 A>C, G>A mutation at chr10:611706…

    Promoter

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGL4.23-IL2RA CaRE6 (minus strand)

    Plasmid

    #91840

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0617)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE6 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    rs7893467 C>A

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL4.23-IL2RA CaRE6 (plus strand)

    Plasmid

    #91839

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0616)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE6 (plus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    rs7893467 G>T, rs11256448 A>G

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL4.23-IL2RA CaRE1 (minus strand)

    Plasmid

    #91838

    Purpose

    Luciferase reporter for IL2RA enhancer (IGI-P0615)

    Depositor

    Jacob Corn

    Article

    Simeonov et al Nature. 2017 Sep 7;549(7670):111-115. do

    Insert

    IL2RA CaRE1 (minus strand) (IL2RA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Perfect match to reference sequence

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. OpenCRISPR-1

    Plasmid

    #221565

    Purpose

    Express OpenCRISPR-1 nuclease in human cells using a CMV promoter

    Depositor

    Ali Madani

    Article

    Ruffolo et al bioRxiv 2024.04.22.590591

    Insert

    OpenCRISPR-1
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pFC330

    Plasmid

    #87842

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    pyrG
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and native pro…

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMAL-his-LbCpf1-EC

    Plasmid

    #79008

    Purpose

    Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    LbCpf1
    

    Use

    Tags

    MBP-6xHis-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAT15068-evoCBE

    Plasmid

    #163013

    Purpose

    plasmid expressing evoCBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    evoCBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAGM47523

    Plasmid

    #153221

    Purpose

    Level 0 zCas9i vector

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    SpCas9 with introns and 2 NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFC332

    Plasmid

    #87845

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pIVT-eeBxb1

    Plasmid

    #232748

    Purpose

    IVT template for making modRNA encoding eeBxb1

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Bxb1
    

    Use

    In vitro transcription

    Tags

    HA and SV40 NLS

    Expression

    Mutation

    V74A, E229K, V375I

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYPQ150

    Plasmid

    #69301

    Purpose

    Gateway entry vector with pcoCas9 (Plant codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pcoCas9 (plant codon-optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mutation

    NLS fusion

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pWS931

    Plasmid

    #123053

    Purpose

    TetR bacterial repressor

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    TetR-NLS [3a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFC333

    Plasmid

    #87844

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and ble selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CMV-enAsCas12f

    Plasmid

    #204637

    Purpose

    Expression of enAsCas12f in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    enAsCas12f
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    D196K, N199K, G276R, N328G, D364R

    Promoter

    CMV

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge