Addgene

We narrowed to 17,141 results for: puro

Showing: 3481 - 3520 of 17141 results

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pCAGGS puro human-GFP-N581K RLIM-IRES-mCherry

Plasmid

#232244

Purpose

Mammalian expression of N581K RLIM-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

N581K RLIM-GFP-IRES-mCherry (RLIM Human)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

N581K

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro -GFP-I77P I78P REX1-IRES-mCherry

Plasmid

#232245

Purpose

Mammalian expression of I77P I78P REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

I77P I78P REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

I77P I78P

Promoter

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-N1-KH12mut (T236D H237D K299D N300D)

Plasmid

#225454

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (T236D H237D K299D N300D)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

T236D H237D K299D N300D

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCW-HA-hRb-delta-CDK-T373D-S608D-S612D-puro

Plasmid

#212675

Purpose

Express tagged hRb phosphosite mutant

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

Rb (RB1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

15 CDK phospho-sites are mutated to alanines, and…

Promoter

TRE

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro cTnT CCND2-T2A-ChloC-P2A-eGFP

Plasmid

#179840

Purpose

donor plasmid for cardiac-specific CCND2-T2A-Chloc-P2A-eGFP expression in human cells

Depositor

Xiaoping Bao

Article

Joshi et al Cells. 2022 Mar 10;11(6). pii: cells1106

Insert

CCND2 (CCND2 Human)

Use

CRISPR and TALEN; Donor plasmid

Tags

chloride-conducting ChR (ChloC) and eGFP

Expression

Mammalian

Mutation

Promoter

Troponin T promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

Plasmid

#221385

Purpose

Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

Depositor

Junwei Shi

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-h.Rhno1 Plk1mut7A-C-PAMmut-Myc-DDK-P2A-Puro

Plasmid

#211616

Purpose

Rhno1 Plk1mut7A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

S51A, T52A, S141A, S162A, S163A, S164A, S178A

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-G3635A-R16-Q2L7-T2176C-UGI-Puro

Plasmid

#209648

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

R16-Q2L7-T2176C

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-h.Rhno1 delta TOPBP1 (T202A)-C-Myc-P2A-Puro

Plasmid

#211619

Purpose

Rhno1 delta TOPBP1 (T202A)

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Use

Tags

myc-flag

Expression

Mammalian

Mutation

T202A

Promoter

CMV

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

Plasmid

#208399

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Baptiste Pradel et al bioRxiv

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

Plasmid

#208398

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Baptiste Pradel et al bioRxiv

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

E2-Crimson

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

Plasmid

#208400

Purpose

codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

Depositor

Raphael Gaudin

Article

Baptiste Pradel et al bioRxiv

Insert

hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)

Use

CRISPR and Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-G3635A-R16-FZY2-S100N-UGI-Puro

Plasmid

#209644

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

R16-FZY2-S100N

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-G3635A-R16-FZY2-S100C-UGI-Puro

Plasmid

#209643

Purpose

To install m.G3635 mutation on mtDNA

Depositor

Bin Shen

Article

Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

Insert

R16-FZY2-S100C

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND1-W86-R17-G1333N-UGI-Puro

Plasmid

#198851

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND1-W86-R17 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND6-W82-R15-G1333C-UGI-Puro

Plasmid

#198861

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND6-W82-R15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND5-W68-R15-G1333N-UGI-Puro

Plasmid

#198859

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND5-W68-R15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND4-W24-R17-G1333N-UGI-Puro

Plasmid

#198857

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND4-W24-R17 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND3-Q26-R19-G1397N-UGI-Puro

Plasmid

#198855

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND3-Q26-R19 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND2-W30-R18-G1333N-UGI-Puro

Plasmid

#198853

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND2-W30-R18 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-CYTB-W77-R14-G1333N-UGI-Puro

Plasmid

#198849

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

CYTB-W77-R14 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX3-W16-R15-G1333N-UGI-Puro

Plasmid

#198847

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX3-W16-R15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX1-W25-R18-G1333N-UGI-Puro

Plasmid

#198843

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX1-W25-R18 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ATP6-W68-R17-G1333N-UGI-Puro

Plasmid

#198839

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP6-W68-R17 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND1-W86-L14-G1333C-UGI-Puro

Plasmid

#198850

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND1-W86-L14 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND5-W68-L15-G1333C-UGI-Puro

Plasmid

#198858

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND5-W68-L15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX3-W16-L17-G1333C-UGI-Puro

Plasmid

#198846

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX3) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX3-W16-L17 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND3-Q26-L18-G1397C-UGI-Puro

Plasmid

#198854

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND3) by swapping Gln codon CAA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND3-Q26-L18 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ATP6-W68-L16-G1333C-UGI-Puro

Plasmid

#198838

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP6) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ATP6-W68-L16 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND4-W24-L18-G1333C-UGI-Puro

Plasmid

#198856

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND4-W24-L18 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND2-W30-L16-G1333C-UGI-Puro

Plasmid

#198852

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND2) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND2-W30-L16 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-CYTB-W77-L15-G1333C-UGI-Puro

Plasmid

#198848

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

CYTB-W77-L15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-COX1-W25-L15-G1333C-UGI-Puro

Plasmid

#198842

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX1-W25-L15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-MTS-ND6-W82-L15-G1333N-UGI-Puro

Plasmid

#198860

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND6-W82-L15 TALEN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PL752-PTPRO-LA-p2a-tdTomato-hGHpA-PGK-puroR-PTPRO-RA

Plasmid

#191686

Purpose

For knocking p2a-tdTomato into PTPRO locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

PTPRO homology arms and p2a-tdTomato

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-CMV-Flag-PolB(K206A/K244A/T304I)-Puro

Plasmid

#177145

Purpose

Lentiviral vector expressing Flag-PolB(K206A/K244A/T304I) and a puromycin resistance cassette

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCT-CMV-copGFP-Polß(L301R/V303R/V306R)-puro

Plasmid

#128666

Purpose

mammalian expression of copGFP-Polß(L301R/V303R/V306R: TM) protein with puromcyin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Flag-Polß(L301R/V303R/V306R)-IRES-puro

Plasmid

#128657

Purpose

mammalian expression of Flag-Polß(L301R/V303R/V306R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
NFATc4-CRISPR-gRNA#exon5-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124290

Purpose

sgRNA against huiman NFATc4

Depositor

Csaba Vizler, Katalin Josvay

Insert

NFATc4 nuclear factor of activated T cells (NFATC4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
NFATc4-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124289

Purpose

sgRNA against human NFATc4

Depositor

Csaba Vizler, Katalin Josvay

Insert

NFATc4 nuclear factor of activated T cells (NFATC4 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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