Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,645 results for: PEP

Clear keywords
Showing: 4351 - 4400 of 4645 results

  2. D9_Ld_190920

    Plasmid
    #135825
    Purpose
    Expression of Leishmania donovani ectodomain in mammalian cells
    Depositor
    Gavin Wright
    Article
    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.
    Insert
    peptidyl-prolyl cis-trans isomerase, macrophage infectivity potentiator precursor, putative
    Tags
    biotinylation sequence and His tag
    Expression
    Mammalian
    Available Since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    135825-plasmid-map-sequence-id-274525

    Enlarge

  11. p028g13-MBP

    Plasmid
    #249631
    Purpose
    Clone MBP polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3
    Depositor
    Isabel Abreu
    Article
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    N-terminal tag with MBP polypeptide
    Expression
    Bacterial
    Mutation
    Silent mutation on Gly-20 to remove a BsaI recogn…
    Available Since
    April 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    249631-plasmid-map-sequence-id-504970

    Enlarge

  16. pLEX307-RAD54L2-D1-86-SFB

    Plasmid
    #247913
    Purpose
    Mammalian expression construct for C-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged RAD54L2 D1-86
    Depositor
    Junjie Chen, Huimin Zhang
    Article
    Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:
    Insert
    RAD54L2 (RAD54L2 Human)
    Use
    Lentiviral
    Tags
    S protein-Flag-Streptavidin binding peptide (SFB)
    Expression
    Mammalian
    Mutation
    deletion of amino acid 1-86
    Available Since
    Dec. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    247913-plasmid-map-sequence-id-494860

    Enlarge

  21. CD55-Delta3

    Plasmid
    #220872
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-3
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-3, short consensus repeat (SCR) domains 1, …
    Promoter
    CMV
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220872-plasmid-map-sequence-id-443043

    Enlarge

  22. CD55-Delta4

    Plasmid
    #220873
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-4
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-4, short consensus repeat (SCR) domains 1, …
    Promoter
    CMV
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220873-plasmid-map-sequence-id-443042

    Enlarge

  23. CD55-Delta1

    Plasmid
    #220870
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-1
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-1, the first short consensus repeat (SCR) d…
    Promoter
    CMV
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220870-plasmid-map-sequence-id-443041

    Enlarge

  24. CD55-Delta2

    Plasmid
    #220871
    Purpose
    Expresses CD55 variant in mammalian cells
    Depositor
    Ferenc Scheeren
    Insert
    CD55 Delta-2
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Delta-2, short consensus repeat (SCR) domains 1 a…
    Promoter
    CMV
    Available Since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220871-plasmid-map-sequence-id-443038

    Enlarge

  25. pET24a MDHM1_ARATH

    Plasmid
    #204236
    Purpose
    Bacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHM1_ARATH (mMDH1 Mustard Weed)
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does done include the first 52 residues that are …
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204236-plasmid-map-sequence-id-413347

    Enlarge

  26. pHR_SFFV*/Puro2a-PAG1-GFP

    Plasmid
    #198223
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Eric Davis
    Insert
    PAG1 (PAG1 Human)
    Use
    Lentiviral
    Tags
    Followed by EGFP, after a flexible linker and Pre…
    Expression
    Mammalian
    Mutation
    None
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198223-plasmid-map-sequence-id-389384

    Enlarge

  29. pKTop::sptse5-CT (1169-1281)

    Plasmid
    #192950
    Purpose
    For membrane insertion study; Expresses sptse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT (1169-1281)
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1281)
    Available Since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192950-plasmid-map-sequence-id-380607

    Enlarge

  31. pKTop::sptse5-CT (1169-1300)

    Plasmid
    #192951
    Purpose
    For membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT (1169-1300)
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for sptse5 (1169-1300)
    Available Since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192951-plasmid-map-sequence-id-380604

    Enlarge

  32. pDR111_mannose_Klf4Gata-3

    Plasmid
    #188401
    Purpose
    Expresses mouse transcription factors Klf4 and Gata-3 in an operon from D-mannose promoter for Bacillus subtilis. The secretion peptide PhoD is fused to both factors.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Inserts
    Klf4 (Klf4 Mouse)
    Gata-3 (Gata3 Mouse)
    Tags
    PhoD secretion peptide
    Expression
    Bacterial
    Mutation
    See Depositor Comments Below
    Promoter
    D-mannose
    Available Since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188401-plasmid-map-sequence-id-370467

    Enlarge

  37. AP2u2-CMV-S_protein

    Plasmid
    #226456
    Purpose
    Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification
    Depositor
    Urartu Seker
    Article
    Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(
    Insert
    Spike Protein
    Tags
    6xHis Tag, Foldon Domain, and Thrombin Cleavage S…
    Expression
    Mammalian
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    226456-plasmid-map-sequence-id-458388

    Enlarge

  40. pLVUT-spC3GFP

    Plasmid
    #225487
    Purpose
    Tet-on lentiviral vector for controlled expression of a secretable and permeable form of C. botulinum exoenzyme C3 transferase fused with EGFP
    Depositor
    Robert Gross
    Article
    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d
    Insert
    Secretable and permeable exoenzyme C3 transferase
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Amino acids 30-251
    Promoter
    Human ubiquitin C
    Available Since
    Sept. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    225487-plasmid-map-sequence-id-444518

    Enlarge

  41. pcDNA3.1-Tras heavy chain-SpyTag003

    Plasmid
    #216293
    Purpose
    Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    Tras heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216293-plasmid-map-sequence-id-433208

    Enlarge

  42. FPR2-DuET

    Plasmid
    #213240
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    FPR2 (FPR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213240-plasmid-map-sequence-id-430798

    Enlarge

  44. pKB235

    Plasmid
    #170024
    Purpose
    Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    CCL20 (CCL20 Escherichia coli, Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Promoter
    Ptac and PvirK
    Available Since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170024-plasmid-map-sequence-id-336757

    Enlarge

  45. pcDNA-GFP-EBA175-Flag(Y1426S)

    Plasmid
    #155035
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    Y1426S; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155035-plasmid-map-sequence-id-309798

    Enlarge

  47. pcDNA-GFP-EBA175-Flag(P1424C)

    Plasmid
    #154978
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424C variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424C; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154978-plasmid-map-sequence-id-309778

    Enlarge

  48. pcDNA-GFP-EBA175-Flag(P1424M)

    Plasmid
    #154979
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424M variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424M; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154979-plasmid-map-sequence-id-310096

    Enlarge

  49. pcDNA-GFP-EBA175-Flag(P1424V)

    Plasmid
    #154988
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424V; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154988-plasmid-map-sequence-id-309963

    Enlarge

  50. pcDNA-GFP-EBA175-Flag(P1424L)

    Plasmid
    #154989
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424L variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424L; insert codes for EBA175 residues 1284 to …
    Promoter
    CMV
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    154989-plasmid-map-sequence-id-309965

    Enlarge

Showing: 4351 - 4400 of 4645 results