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pSI-Check2-hRluc-NFkB-firefly

Plasmid

#106979

Purpose

Luciferase reporter with internal control for NFkB signal.

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Insert

NFKB recognition sites (NFKB1 Human)

Use

Luciferase

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTXB1-pA-M.EcoGII

Plasmid

#192873

Purpose

pA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578

Depositor

Chong Tang

Article

Weng et al Genome Biol. 2023 Mar 29;24(1):61. doi:

Insert

pA-M.EcoGII

Tags

3X Flag Tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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polyQ74-GFP

Plasmid

#231893

Purpose

Forms cytosolic HTT partial exon 1 Q74 aggregates

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

HTT partial exon 1 Q74 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

CAG repeat expansion

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_Ef1a:PE2-P2A-hMLH1dn

Plasmid

#184444

Purpose

Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

Depositor

Bobby Koeleman

Article

Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and Ef1A

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-mcBEST

Plasmid

#209416

Purpose

Plasmid for multiplexed cytosine base editing in streptomycetes

Depositor

Tilmann Weber

Article

Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

Inserts

Codon optimized APOBEC1-nCas9-UGI fusion protein
csy4 from Pseudomonas aeruginosa PAO1

Use

CRISPR and Synthetic Biology

Promoter

base editing cassette: PtipA ; sgRNAs: PkasO* an…

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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tau exon 9–12 splicing minigene

Plasmid

#121165

Purpose

Generates circular RNAs from a Tau minigene

Depositor

Stefan Stamm

Article

Welden et al Biochim Biophys Acta Mol Basis Dis. 2018

Insert

Tau exons 9–12 (MAPT Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPR-aBEST

Plasmid

#131464

Purpose

Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

Inserts

Streptomyces codon optimized spCas9n (D10A)
streptomyces codon optimized tadA

Use

CRISPR and Synthetic Biology

Promoter

tipA

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti_Ef1a:PE2-P2A-GFP

Plasmid

#184445

Purpose

Single EF1a-driven PE2-P2A-GFP (Addgene #132776) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

Depositor

Bobby Koeleman

Article

Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and Ef1A

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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alpha-0 CFP in pcDNA3.1

Plasmid

#36396

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted into the KpnI-SpeI sites engineered …

Promoter

cmv

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pT2KXIG (Tol2)-tp63:ikk1:GFP

Plasmid

#105646

Purpose

Ikk1(alpha) expression in basal keratinocytes

Depositor

Sandra Rieger

Article

Lisse et al J Cell Sci. 2017 Mar 1;130(5):975-988. d

Insert

tp63:ikk1:GFP (chuk Zebrafish)

Tags

GFP

Expression

Mammalian

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pUS2-SH231

Plasmid

#115150

Purpose

Cas9-GFP, dual gRNA expression vector for targeted integration of repair templates into safe harbor 231 locus in chromosome 4

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Dual SH231 gRNAs

Use

CRISPR

Tags

3xFLAG-SV40NLS, GFP, and Nucleoplasmin NLS

Expression

Mammalian

Promoter

U6, CBh

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL Blast Twist ER

Plasmid

#18799

Depositor

Bob Weinberg

Article

Mani et al Cell. 2008 May 16. 133(4):704-15.

Insert

twist (Twist1 Mouse)

Use

Retroviral

Tags

ER hormone binding domain

Expression

Mammalian

Mutation

G525R mutation in the ER. The presence of the G52…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pCAT077

Plasmid

#180511

Purpose

Plasmid expressing mammalian codon optimized wt PlmCasX, sgRNAv2 scaffold, and restriction sites to clone in new spacers

Depositor

Jennifer Doudna

Article

Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

Inserts

CasX sgRNAv2
PlmCasX

Use

CRISPR

Tags

2x FLAG and SV40 NLS

Expression

Mammalian

Promoter

CAG and U6

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

Plasmid

#190153

Purpose

Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp2 (Vamp2 Mouse)

Use

AAV

Tags

P2AT2A-EGFP-caax

Expression

Mammalian

Mutation

Truncation that includes only AA #1-94

Promoter

MBP

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB delta CDK + T373

Plasmid

#58912

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T373 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S612

Plasmid

#58914

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S612 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S612 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S608

Plasmid

#58913

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S608 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S608 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

Plasmid

#105643

Purpose

Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

Depositor

Sandra Rieger

Article

Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

Insert

Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

Expression

Mammalian

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only
mito-yHS1-M7A

Plasmid

#159164

Purpose

Mitochondrial labile heme reporter

Depositor

Amit Reddi

Article

Martinez-Guzman et al J Cell Sci. 2020 May 20;133(10). pii: jc

Insert

mito-yHS1-M7A

Tags

COX4 pre-sequence

Expression

Yeast

Mutation

Mutated Met 7 of the cyt b562 module of mito-yHS1…

Promoter

GPD

Available Since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MTRAP-bio

Plasmid

#68530

Purpose

Expresses full-length P. vivax MTRAP ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

MTRAP

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-LAT Y6F YYYY-LAT YYYY -mCitrine (XSB588)

Plasmid

#78516

Purpose

Lentiviral vector for expressing LAT-Y6F YYYY-LAT-YYYY-mCitrine (3x2 Grb2 binding sites)

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

LAT (LAT Human)

Use

Lentiviral

Tags

mCitrine

Expression

Mammalian

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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MSP1-bio

Plasmid

#68505

Purpose

Expresses full-length P. vivax MSP1 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

MSP1

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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PVX_110945-bio

Plasmid

#68538

Purpose

Expresses full-length P. vivax PVX_110945 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

PVX_110945

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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RIPR-bio

Plasmid

#68526

Purpose

Expresses full-length P. vivax RIPR ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

RIPR

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
DBP-bio

Plasmid

#68528

Purpose

Expresses full-length P. vivax DBP ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

DBP

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + S780

Plasmid

#58915

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S780 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S780 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
MSP5-bio

Plasmid

#68511

Purpose

Expresses full-length P. vivax MSP5 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

MSP5

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV HA hRB delta CDK + S811

Plasmid

#58919

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S811 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S811 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
CyRPA-bio

Plasmid

#68525

Purpose

Expresses full-length P. vivax CyRPA ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence

Depositor

Julian Rayner, Gavin Wright

Article

Hostetler et al PLoS Negl Trop Dis. 2015 Dec 23;9(12):e0

Insert

CyRPA

Tags

rat CD4 d3+4, enzymatic biotinylation sequence

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV HA hRB delta CDK + S807

Plasmid

#58918

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S807 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S807 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only