We narrowed to 5,258 results for: pcdna3.1

1. pCDNA-SCW

   Plasmid

   #190633

   Purpose

   Mammalian expression of genetically encoded calcium indicator SCW

   Depositor

   Robert Campbell

   Article

   Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

   Insert

   SCW
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA PRAS40 F129A

   Plasmid

   #15673

   Depositor

   John Lawrence

   Article

   Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

   Insert

   PRAS40 F129A (AKT1S1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F129A

   Promoter

   Available since

   Sept. 24, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pTS2404-Tier1-PhCMV-NeoR

   Plasmid

   #169496

   Purpose

   Tier-1 vector encoding PhCMV-driven neomycin resistance gene NeoR (PhCMV-NeoR-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven aminoglycoside 3'-phosphotransferase, APH 3' II
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-HA-ERK2 L198A

   Plasmid

   #8975

   Depositor

   John Blenis

   Article

   Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

   Insert

   ERK2 (Mapk1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   L198A

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

5. nonphosphorylatable Cenp-B-targted Aurora B FRET sensor

   Plasmid

   #45230

   Depositor

   Michael Lampson

   Article

   Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

   Insert

   nonphos Cenp-B targeted Aurora B FRET sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   t-a mutation in substrate sequence

   Promoter

   Available since

   July 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CMV-2xFLAG-Atoh1ER

   Plasmid

   #101736

   Purpose

   Expresses mouse Atoh1 fused to ER

   Depositor

   Albert Edge

   Article

   Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

   Insert

   Atoh1 (Atoh1 Mouse)
   

   Use

   Tags

   2x FLAG and ER

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SEPLuc-IRES-Antares

   Plasmid

   #141085

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   SEPLuc-EMCV IRES-Antares
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mGFAP R236H

   Plasmid

   #169475

   Purpose

   Single p.Arg236His(R236H) mutation was individually inserted by site-directed mutagenesis into the wild-type mGFAP

   Depositor

   Federico Herrera

   Article

   Letra-Vilela et al Experimental Results (2020), Volume 1, e

   Insert

   HaloTAG mGFAP (R236H) (Gfap Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed R 236 to H

   Promoter

   Available since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmyc-GFP-TNRC6C-(928-1020)-NES-mut

   Plasmid

   #42027

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6C (TNRC6C Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   contain aa928-1020, L943A, L947A, M950A, F952A

   Promoter

   CMV

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBBJ

    Plasmid

    #62672

    Purpose

    Vector for FLPo mRNA synthesis

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    FLPo
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-FLAG-Fos Y345A

    Plasmid

    #8970

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y345A

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

12. Vh-HA-FRB

    Plasmid

    #20225

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    T2098L mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

13. Septin 11 isoform II

    Plasmid

    #120315

    Purpose

    Septin 11 isoform II mammalian expression

    Depositor

    Angel de Blas

    Article

    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

    Insert

    SEPT11 (Sept11 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOVC1_X_VfAU1-LOV

    Plasmid

    #131185

    Purpose

    Expresses VfAU1-LOV domain (Vaucheria frigida) preceded by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    VfAU1-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOVC1_VfAU1-LOV_X

    Plasmid

    #131174

    Purpose

    Expresses VfAU1-LOV domain (Vaucheria frigida) followed by restriction sites

    Depositor

    Harald Janovjak

    Article

    Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

    Insert

    VfAU1-LOV
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNLRP11minor.MYC

    Plasmid

    #131195

    Purpose

    Mammalian expression of human NLRP11 188A

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

    Insert

    NLRP11 188A (NLRP11 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    188A

    Promoter

    pCMV

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Antares-SEPLuc

    Plasmid

    #141086

    Purpose

    precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-EMCV IRES-SEPLuc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 66 to 91 on the Luciferas…

    Promoter

    CMV

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-FLAG-Fos F343A

    Plasmid

    #8969

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F343A

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

19. 3XFLAG-NSarQ27G-VMAn-FKBP

    Plasmid

    #70229

    Purpose

    Expresses FLAG tagged N-terminal portion of alpha sarcin with Q27G mutation along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (N-terminal portion)
    

    Use

    Tags

    3XFLAG, FK506- and rapamycin-binding protein (FKB…

    Expression

    Mammalian

    Mutation

    Changed Gln 27 to Gly

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

20. HA PRAS40 P185A

    Plasmid

    #15675

    Depositor

    John Lawrence

    Article

    Wang et al J Biol Chem. 2007 Jul 6. 282(27):20036-4

    Insert

    PRAS40 P185A (AKT1S1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    P185A

    Promoter

    Available since

    Sept. 24, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA3-FLAG-Fos DD F343A

    Plasmid

    #8972

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S362D, S374D, F343A

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3-FLAG-Fos DD Y345A

    Plasmid

    #8971

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S362D, S374D, Y345A

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

23. CaMK2rep

    Plasmid

    #239622

    Purpose

    CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.

    Depositor

    F Javier Díez Guerra

    Insert

    SpotNES-sGFP2-SynP3-3xmyc (Syn1 Synthetic, Rat)
    

    Use

    Tags

    Spot, myc, and sGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. mproSAAS (27 kDa)

    Plasmid

    #231633

    Purpose

    Mammalian expression of the full-length mouse chaperone protein proSAAS.

    Depositor

    Iris Lindberg

    Article

    Fortenberry et al J Biol Chem. 2002 Feb 15;277(7):5175-86.

    Insert

    Pcsk1N (Pcsk1n Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. eGFP-MYO5C

    Plasmid

    #239278

    Purpose

    Expression of N terminal GFP tagged Myo5C

    Depositor

    David Pellman

    Article

    Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

    Insert

    MYO5C (MYO5C Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. hPDZD11-HA

    Plasmid

    #236537

    Purpose

    For expression of PDZD11 (human) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Guerrera et al J Biol Chem. 2016 May 20;291(21):11016-2

    Insert

    PDZD11 (PDZD11 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. cASIC1 concatemer - fully assembled

    Plasmid

    #233133

    Purpose

    To create cASIC1 channels of defined stoichiometry in mammalian cells

    Depositor

    David MacLean

    Article

    Purohit et al Biophys J. 2024 Oct 15;123(20):3507-3518

    Insert

    ASIC1 (ASIC1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Const.2 EGFP-P2A-dTomato-PS9

    Plasmid

    #231906

    Purpose

    For packaging EGFP and dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs)

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP-P2A-dTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CLIPf-KIF5A(1-573)

    Plasmid

    #219683

    Purpose

    Mammalian expression of CLIPf-KIF5A(1-573)

    Depositor

    Alison Twelvetrees

    Article

    Smith et al bioRxiv 2024.12.20.629623

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    CLIPf

    Expression

    Mammalian

    Mutation

    1-573

    Promoter

    CMV & T7

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSCD-HCVP

    Plasmid

    #226227

    Purpose

    Express HCVP

    Depositor

    Michael Elowitz

    Article

    Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

    Insert

    HCVP (POLY Hepatitis C virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. TRAPa(R150A)-3xFLAG-pCDNA

    Plasmid

    #201966

    Purpose

    Mammalian expression of mutated Translocon-associated protein subunit alpha

    Depositor

    Ville Paavilainen

    Article

    Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

    Insert

    SSR1 (SSR1 Human)
    

    Use

    Tags

    3xFLAG-tag

    Expression

    Mammalian

    Mutation

    R150A

    Promoter

    Available since

    Jan. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFH2.13_GagZip-MCP_Zip-P2A-eGFP

    Plasmid

    #205528

    Purpose

    Express HIV GagZip-MCP_Zip-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    GagZip-MCP_Zip
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Nucleocapsid replaced with leucine zipper

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mc2 blast hPGK

    Plasmid

    #206229

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mc2 hygro hPGK

    Plasmid

    #206228

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. mc2 puro hPGK

    Plasmid

    #206227

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mc2 neo hPGK

    Plasmid

    #206226

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mc2 hygro EF1a

    Plasmid

    #206222

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. mc2 hygro

    Plasmid

    #206216

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pB2AR-miRFP718nano

    Plasmid

    #195751

    Purpose

    Beta-2-adrenergic receptor internally tagged with miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    B2AR-miRFP718nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGas-miRFP718nano

    Plasmid

    #195752

    Purpose

    G protein alpha-subunit internally tagged with miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    G protein alpha-s-miRFP718nano
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge