We narrowed to 4,562 results for: TIL
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Plasmid#170896PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT9
Plasmid#170897PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT10
Plasmid#170898PurposeGoldenBraid Double Transcriptional Unit - pCmYLCV911_E-Luc_NtEUt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpCmYLCV911Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT12
Plasmid#170900PurposeGoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…Promoterp35SoTMVAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT13
Plasmid#170901PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT14
Plasmid#170902PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_AtACT2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-4
Plasmid#163142PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the BatLPMO10 signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-3
Plasmid#163141PurposepLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-2
Plasmid#163140PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCVbsd-PPARD-K421R-K422R
Plasmid#130778PurposeVector encoding PPARD cDNA for retroviral transductionDepositorAvailable SinceSept. 16, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVbsd-PPARD-T423D-T427A
Plasmid#130792PurposeVector encoding PPARD cDNA for retroviral transductionDepositorAvailable SinceSept. 16, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
Antares-SEPLuc IRESv24
Plasmid#141087Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRESDepositorInsertAntares-T2A-puromycin-IRESv24-SEPLuc
ExpressionMammalianPromoterCMVAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCVbsd-PPARD-K421A-K422A
Plasmid#130777PurposeVector encoding PPARD cDNA for retroviral transductionDepositorAvailable SinceOct. 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVbsd-PPARD-H413W-T427I
Plasmid#130766PurposeVector encoding PPARD cDNA for retroviral transductionDepositorAvailable SinceOct. 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVbsd-PPARD-C91A-E92A
Plasmid#130726PurposeVector encoding PPARD cDNA for retroviral transductionDepositorAvailable SinceOct. 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVbsd-PPARD-T423D-T427D
Plasmid#130790PurposeVector encoding PPARD cDNA for retroviral transductionDepositorAvailable SinceAug. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVbsd-PPARD-R314A-H413W
Plasmid#130764PurposeVector encoding PPARD cDNA for retroviral transductionDepositorAvailable SinceAug. 28, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVbsd-PPARD-T261R-V279R
Plasmid#130739PurposeVector encoding PPARD cDNA for retroviral transductionDepositorAvailable SinceAug. 27, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVbsd-PPARD-H413W-K421A-K422A
Plasmid#130765PurposeVector encoding PPARD cDNA for retroviral transductionDepositorAvailable SinceAug. 27, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVbsd-PPARD-T423A-T427A
Plasmid#130789PurposeVector encoding PPARD cDNA for retroviral transductionDepositorAvailable SinceAug. 27, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCVbsd-PPARD-T423A-T427D
Plasmid#130791PurposeVector encoding PPARD cDNA for retroviral transductionDepositorAvailable SinceAug. 27, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCCL/IFNB1-d2eGFP-3’UTR
Plasmid#180232PurposeReporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1DepositorInsertd2eGFP
UseLentiviralPromoter1 kb upstream of IFNB1 CDSAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST
Plasmid#125689PurposeC to T base editor for actinomycetesDepositorInsertstreptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
UseCRISPR and Synthetic BiologyPromoterermE*/tipAAvailable SinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
MLNR-DuET
Plasmid#213339PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHalo-MyosinIIB-C25
Plasmid#128574PurposeStudies of cellular contractility.DepositorAvailable SinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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LifeAct-14 (LA-14)
Plasmid#158750PurposeLentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.DepositorInsertLifeAct-14
UseLentiviralTagseGFPExpressionMammalianMutationDeletion mutation for last three amino acids from…PromoterCMV immediate earlyAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-cBEST-v2-PermE
Plasmid#209446Purposeimproved pCRISPR-cBEST plasmid with optimized sgRNA cloning siteDepositorInsertCodon optimized APOBEC1-nCas9-UGI fusion protein
UseCRISPR and Synthetic BiologyPromoterPtipA; sgRNA expression from ermE* promoterAvailable SinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-NAP1
Plasmid#208862PurposeStably express GFP-tagged NAP1 for CID induced mitophagyDepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNG1256
Plasmid#149710PurposeExpresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complexDepositorAvailable SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMI91
Plasmid#74888PurposeShuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more informationDepositorInsertTitin I27 x 9 (TTN Human)
TagsHis-tag and Strep-tag IIExpressionBacterialPromoterT7 promoterAvailable SinceJune 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGL1252_TetOFF(Hyg)
Plasmid#118992PurposeTetOFF (promoter-less) cassette with Hygromycin selectionDepositorInsertstTA+Tnos
Hygromycin cassette
Ttub+PtetO
UseFungal expression (in magnaporthe oryzae)MutationCodon optimized for Magnaporthe oryzaePromoterAspergillus nidulans TrpC promoter, Ustilago mayd…Available SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-12xLexAOp-mCherry-MiniWhite
Plasmid#165917PurposeBlasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable LexAOp Response Vector
UseSynthetic BiologyAvailable SinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
OmEF1aCas9P2ApuroSB
Plasmid#165484PurposeStable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cellsDepositorInsertZebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
UseCRISPR; TransposonPromoterOreochromis mossambicus EF1 alpha promoter (OmEF1…Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
SEP-Nanoluc-cyOFP1
Plasmid#141082Purposeprototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosisDepositorInsertSEP-Nanoluc-cyOFP1
UseLentiviralMutationDeletion of amino acids DISGG from positions 673 …PromoterEF1αAvailable SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-GMRWhite
Plasmid#165907PurposeBlasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
XW17
Plasmid#65835PurposePunc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
TagsmCherryExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…Available SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0-iRFP670-micro
Plasmid#135954PurposeExpresses an iLID micro (SspB R73Q)-iRFP670 fusion in mammalian cells.DepositorInsertiLID micro (SspB R73Q)
UseLentiviralTagsiRFP670ExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-MiniWhite
Plasmid#165910PurposeBlasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
ama1CD4d3+4-blac
Plasmid#36146PurposeEXPRESs plasmid for P. falciuparum merozoite surface proteins encoding P. falciparum ama1 with rat CD4d3+4-blacDepositorInsertama1 (PF3D7_1133400 Plasmodium falciparum)
Tagsbeta-lactamase, pentamerization domain of rat car…ExpressionMammalianPromoterCMVAvailable SinceMay 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSR06
Plasmid#69152Purposeread-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr IIDepositorInsertsmCherry
eGFP
UseCre/Lox; MossciExpressionWormMutationcodon-optimzed index 1.0 and codon-optimzed index…Promoterrps-27Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-mCherry-MiniWhite
Plasmid#165911PurposeBlasticidin resistant 5xUAS mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc
Plasmid#129105PurposeExpresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone productionDepositorInsertBsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
ExpressionBacterialAvailable SinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBlastR-12xLexAOp-EBFP2-MiniWhite
Plasmid#165918PurposeBlasticidin resistant 12xLexAOp EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable LexAOp Response Vector
UseSynthetic BiologyAvailable SinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)
Plasmid#208869PurposeFor mammalian expression of NAP1 (delta-TBK1)DepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-GST-TEV-GFP-NAP1
Plasmid#208870PurposeFor expression in E. coli of GFP-NAP1DepositorAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-EBFP2-GMRWhite
Plasmid#165921PurposeBlasticidin resistant 5xQUAS EBFP2 GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-sfGFP-MiniWhite
Plasmid#165922PurposeBlasticidin resistant 5xQUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xQUAS-EBFP2-MiniWhite
Plasmid#165924PurposeBlasticidin resistant 5xQUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable QUAS Response Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-EBFP2-MiniWhite
Plasmid#165912PurposeBlasticidin resistant 5xUAS EBFP2 Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only