We narrowed to 7,572 results for: Lif

1. CVS-N2c-jGCaMP8m

   Plasmid

   #172388

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   GCaMP8m
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-mDLX-TVA-2A-N2cG

   Plasmid

   #172364

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neurons

   Depositor

   Peter Jonas, Yoav Ben-Simon

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mDLX

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJCC_076 AsCas12a R1226A

   Plasmid

   #171669

   Purpose

   For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   AsCas12a R1226A
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed arginine 1226 to alanine

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

   Plasmid

   #172535

   Purpose

   Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

   Depositor

   Richard White

   Article

   Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

   Insert

   -3.5ubb:plin2-tdtomato
   

   Use

   Tags

   Expression

   Mutation

   S80R, A81S, E144D, I148F and R415H mutations obse…

   Promoter

   zebrafish ubb

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMAL_dstβL

   Plasmid

   #73805

   Purpose

   Plasmid for selection for insertion and homodimerization

   Depositor

   Sarel Fleishman

   Article

   Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi:

   Inserts

   CAT Marker
   ToxR
   beta lactamase
   spectomycin resistance
   CLS TM domain
   

   Use

   Tags

   Part Of the dstbl chiemra, Part of the dsTbL chim…

   Expression

   Bacterial

   Mutation

   Promoter

   NaN and ToxR promotor

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pBXNPH3

   Plasmid

   #110098

   Purpose

   E.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag and 3C cleavage site

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Type

   Empty backbone

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-EGFP barcode-5-SV40 polyA

   Plasmid

   #190869

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode5
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH3626

   Plasmid

   #179630

   Purpose

   Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   RFP

   Expression

   Worm

   Mutation

   Promoter

   Pacr-2(s)

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-EF1-IRES-NEO mutant ITR

   Plasmid

   #67905

   Purpose

   PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

   Depositor

   Alex Kentsis

   Article

   Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

   Insert

   NeoR (neoR Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CVS-N2c-nl.mCherry-FlpO

    Plasmid

    #172378

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    nuclear-localized mCherry + FlpO
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac1-Ac-AChBP

    Plasmid

    #63128

    Purpose

    Expression of Aplysia californica AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

12. P447-FBP_19

    Plasmid

    #139710

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsive

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SAC1C389SdeltaTMD-FKBP-mCherry

    Plasmid

    #108124

    Purpose

    Recruitable cytosolic inactive SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(C389S; 1-520):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(C389S; 1-520)

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)

    Plasmid

    #193853

    Purpose

    Promoterless insertion vector for split HygR/mScarlet-I landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' rps-0::HygR + 5' mScarlet-I
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-0p

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. P445-FBP_17

    Plasmid

    #139708

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlled

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. VinTS-tCRMod-GGSGGS5

    Plasmid

    #111763

    Purpose

    Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module

    Depositor

    Brenton Hoffman

    Article

    LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

    Insert

    Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. C204

    Plasmid

    #174358

    Purpose

    For expressing siRNA-resistant human PABPC1 in human cell lines via transfection

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (PABPC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Multiple silent mutations introduced to be resist…

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPI226

    Plasmid

    #113228

    Purpose

    safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-mCherry

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Insert

    LifeAct
    

    Use

    Safe haven knock-in plasmid for the act5 locus, l…

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    act5

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. px552-sg-gria1-HT-SEP

    Plasmid

    #187653

    Purpose

    Contains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.

    Depositor

    Erin O'Shea

    Article

    Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

    Insert

    HaloTag-SEP donor
    

    Use

    AAV and CRISPR

    Tags

    N/A

    Expression

    Mutation

    Promoter

    N/A

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-Pou4f3

    Plasmid

    #158530

    Purpose

    Expresses human Pou4f3 in mammalian cells

    Depositor

    Neil Segil

    Article

    Menendez et al Elife. 2020 Jun 30;9. pii: 55249. doi: 1

    Insert

    POU Class 4 Homeobox 3 (POU4F3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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