We narrowed to 29,405 results for: des.2

1. pPGK-T7/2-CD44v6-10 (fl)

   Plasmid

   #137820

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v6-10 (fl) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length

   Promoter

   PGK

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHiFi Cas9-2×sgRNA (empty, donor)

   Plasmid

   #162277

   Purpose

   All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.

   Depositor

   Kazuhisa Nakayama

   Article

   Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi

   Insert

   HiFi Cas9
   

   Use

   CRISPR

   Tags

   BPNLS and FLAG tag

   Expression

   Mammalian

   Mutation

   SpCas9 (R691A)

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

   Plasmid

   #169186

   Purpose

   To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

   Depositor

   John Diffley

   Article

   Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

   Insert

   14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)
   

   Tags

   14His-SUMO (Ulp1-cleavable)

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUFlip-floxed2A-mRFP-; gcry1:BFP -2

   Plasmid

   #173888

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; gcry1: BFP
   

   Use

   Synthetic Biology

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 SARS-CoV-2 S D614

   Plasmid

   #158074

   Purpose

   Expresses SARS-CoV-2 spike protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX307-SARS-CoV-2-3CL-C145A

   Plasmid

   #160279

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   C145A

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. JWW-2 human chimeric monoclonal antibody

   Plasmid

   #66749

   Purpose

   Expresses a murine/human chimeric IgG1 HPV16 L2-specific neutralizing antibody that recognizes HPV16 L2 amino acid region 58-64 . JWW-2 works in ELISA/WB/HPV

   Depositor

   Richard Roden

   Article

   Wang et al Clin Vaccine Immunol. 2015 May 13. pii:

   Inserts

   JWW-2 Heavy Chain
   JWW-2 Light Chain
   

   Expression

   Mammalian

   Promoter

   mEF1 and rEF1

   Available Since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mouse Alpha2 delta-2 HA pMT2

   Plasmid

   #58731

   Purpose

   expression of HA tagged mouse Alpha2 delta-2 in mamalian cells

   Depositor

   Annette Dolphin

   Article

   Davies et al J Neurosci. 2006 Aug 23;26(34):8748-57.

   Insert

   alpha2 delta-2 (Cacna2d2 Mouse)
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. FH1-MGT#2-mCherry_PGK-H2B-CFP

   Plasmid

   #164102

   Purpose

   Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

   Depositor

   Gaetano Gargiulo

   Article

   Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

   Insert

   MGT#2-mCherry
   

   Use

   Lentiviral and Synthetic Biology

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2xFlag CMV2 Lats1-PY1+2 mutant

    Plasmid

    #18975

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

    Insert

    Large tumor suppressor kinase 1 PY1+2 mutant (LATS1 Human)
    

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    PY1 mutant, (Tyrosine 376 changed to Alanine) and…

    Available Since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pBiFC-HA-hCaspase 2 prodomain-VC155

    Plasmid

    #49261

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 prodomain (CASP2 Human)
    

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    delete aa 148-435

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pBIFC-flag-hCaspase 2 prodomain-VN173

    Plasmid

    #49262

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 prodomain (CASP2 Human)
    

    Tags

    flag and split Venus N

    Expression

    Mammalian

    Mutation

    delete aa 148-435

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pBiFC-HA-hCaspase 2 CARD-VC155

    Plasmid

    #49259

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    Caspase-2 CARD (CASP2 Human)
    

    Tags

    HA and split Venus C

    Expression

    Mammalian

    Mutation

    deleted aa 123-435

    Promoter

    CMV

    Available Since

    Dec. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pBiFC-flag-hCaspase 2 CARD-VN173

    Plasmid

    #49260

    Purpose

    Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

    Depositor

    Douglas Green

    Article

    Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

    Insert

    caspase-2 CARD (CASP2 Human)
    

    Tags

    Flag and split Venus N

    Expression

    Mammalian

    Mutation

    delete aa 123-435

    Promoter

    CMV

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

15. TET-pLKO.1 PURO shID2 #2

    Plasmid

    #83087

    Purpose

    Lentiviral shRNA vector for inducible knockdown of human ID2

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shID2 (ID2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

16. pPGK-T7/2-CD44v6-10 (-cyt)

    Plasmid

    #137821

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v6-10 (-cyt) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region

    Promoter

    PGK

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA4TO R99A CoV-2 nsp1 3xFLAG

    Plasmid

    #176061

    Purpose

    Expresses R99A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG fusion to nsp1.

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    CoV-2 nsp1 (ORF1ab SARS-CoV-2)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LSB-hsa-let-7a-2-3p

    Plasmid

    #103144

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-let-7a-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-let-7a-2-3p target (MIRLET7A2 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. Anti-SCG10/Stathmin-2 [L5/1R]

    Plasmid

    #140065

    Purpose

    Mammalian Expression Plasmid of anti-SCG10/Stathmin-2 (Rat). Derived from hybridoma L5/1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-SCG10/Stathmin-2 (Rat) recombinant mouse monoclonal antibody (Stmn2 Mouse)
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. LSB-hsa-miR-26a-2-3p

    Plasmid

    #103378

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-26a-2-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-26a-2-3p target (MIR26A2 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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