We narrowed to 5,784 results for: pcas

1. pCAGmRuby2smFPMyc

   Plasmid

   #71816

   Purpose

   encodes multiple copies of an epitope tag Myc ( not detected by anti GFP ab)

   Depositor

   Loren Looger

   Insert

   mRuby2_smFP_Myc
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG:Kaede

   Plasmid

   #39261

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

   Insert

   Kaede
   

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   Nov. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCas_2+5

   Plasmid

   #60594

   Purpose

   encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cas_2+5
   

   Expression

   Bacterial

   Available Since

   April 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGImC_Empty

   Plasmid

   #92015

   Purpose

   Empty backbone, includes IRES-mCherry

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Type

   Empty backbone

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas1_Cas2/3

   Plasmid

   #89240

   Purpose

   Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas1 and Cas2/3
   

   Tags

   Strep II tag on Cas1

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9CR4-NG

   Plasmid

   #120225

   Purpose

   pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.

   Depositor

   Christopher Reisch

   Insert

   SpCas9-NG
   

   Use

   Synthetic Biology

   Tags

   ssrA

   Mutation

   Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

   Promoter

   pTet

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMBPCas2/3

   Plasmid

   #89238

   Purpose

   Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

   Depositor

   Blake Wiedenheft

   Article

   Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

   Insert

   Cas2/3
   

   Tags

   Strep II-MBP

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAD64_pCMV6_Entry_hIrg1

   Plasmid

   #124879

   Purpose

   Expresses hCAD His103Ala mutant in mammalian cells

   Depositor

   Konrad Buessow

   Article

   Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

   Insert

   cis-aconitate decarboxylase (ACOD1 Human)
   

   Tags

   myc and FLAG tags

   Expression

   Mammalian

   Mutation

   mutated His103 to Ala

   Promoter

   CMV

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas3-Sm

   Plasmid

   #208001

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   

   Expression

   Mammalian

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas49

    Plasmid

    #82391

    Purpose

    rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    rbcL-GFP
    

    Promoter

    PccmK2

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCaryP

    Plasmid

    #18945

    Depositor

    Michele Calos

    Article

    Groth et al Genetics. 2004 Apr . 166(4):1775-82.

    Insert

    phiC31 attP
    

    Expression

    Insect

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCas4_1_2_VA

    Plasmid

    #186438

    Purpose

    Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas4, Cas1 and Cas2
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAM12

    Plasmid

    #86471

    Purpose

    Expresses p12 and p9-mCitrine

    Depositor

    Pamela Silver

    Article

    Myhrvold et al ACS Synth Biol. 2016 Aug 3.

    Insert

    p12, p9-mCitrine
    

    Use

    Synthetic Biology

    Tags

    mCitrine

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    April 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG2IG

    Plasmid

    #122292

    Purpose

    Overexpression control

    Depositor

    Yukiko Gotoh

    Article

    Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAT024

    Plasmid

    #171636

    Purpose

    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BFP
    mTagBFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. M-SPcas

    Plasmid

    #48668

    Purpose

    Mammalian S. pyogenes Cas9 expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    NLS

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAM17

    Plasmid

    #86473

    Purpose

    Expresses p12 and p9

    Depositor

    Pamela Silver

    Article

    Myhrvold et al ACS Synth Biol. 2016 Aug 3.

    Insert

    p12, p9
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpCas9_human_ADAR2_ccC

    Plasmid

    #158112

    Purpose

    deletion hADAR2

    Depositor

    Emanuela Felley-Bosco

    Article

    Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

    Insert

    ADAR2 (ADARB1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS_2paLINX

    Plasmid

    #129655

    Purpose

    2P-activatable light-inducible nuclear exporter

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paLINX
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG_TWIST1

    Plasmid

    #215027

    Purpose

    Mammalian expression of human TWIST1

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    twist family bHLH transcription factor 1 (TWIST1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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