We narrowed to 5,878 results for: pcas

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pCAG:Kaede

Plasmid

#39261

Depositor

Anna-Katerina Hadjantonakis

Article

Nowotschin et al BMC Dev Biol. 2009 Sep 9;9:49.

Insert

Kaede

Expression

Mammalian

Promoter

pCAGGS

Available Since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pCA_e-2

Plasmid

#202097

Purpose

Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGImC_Empty

Plasmid

#92015

Purpose

Empty backbone, includes IRES-mCherry

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Tags

V5

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCas1_Cas2/3

Plasmid

#89240

Purpose

Plasmid contains the genes Cas1 and Cas2/3, cloned from Pseudomonas aeruginosa as a polycistronic operon. Cas1 includes an N-terminal Strep II tag and TEV protease cleavage site on Cas1

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

Cas1 and Cas2/3

Tags

Strep II tag on Cas1

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCas_2+5

Plasmid

#60594

Purpose

encodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2

Depositor

Christopher Voigt

Article

Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

Insert

Cas_2+5

Expression

Bacterial

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pCas9CR4-NG

Plasmid

#120225

Purpose

pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.

Depositor

Christopher Reisch

Insert

SpCas9-NG

Use

Synthetic Biology

Tags

ssrA

Mutation

Mutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…

Promoter

pTet

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pCAD64_pCMV6_Entry_hIrg1

Plasmid

#124879

Purpose

Expresses hCAD His103Ala mutant in mammalian cells

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

myc and FLAG tags

Expression

Mammalian

Mutation

mutated His103 to Ala

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMBPCas2/3

Plasmid

#89238

Purpose

Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

Cas2/3

Tags

Strep II-MBP

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCaryP

Plasmid

#18945

Depositor

Michele Calos

Article

Groth et al Genetics. 2004 Apr . 166(4):1775-82.

Insert

phiC31 attP

Expression

Insect

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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M-SPcas

Plasmid

#48668

Purpose

Mammalian S. pyogenes Cas9 expression, human optimized

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Cas9

Use

CRISPR

Tags

NLS

Promoter

CMV

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pCas3-Sm

Plasmid

#208001

Purpose

Expresses all necessary components of Type I-C CRISPR-Cas system

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Inserts

RhaR (rhaR )
RhaS (rhaS )
pRhaBAD
I-C crRNA
Cas3
Cas5
Cas8

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCAM12

Plasmid

#86471

Purpose

Expresses p12 and p9-mCitrine

Depositor

Pamela Silver

Article

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p12, p9-mCitrine

Use

Synthetic Biology

Tags

mCitrine

Expression

Bacterial

Promoter

pBAD

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG2IG

Plasmid

#122292

Purpose

Overexpression control

Depositor

Yukiko Gotoh

Article

Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

Type

Empty backbone

Expression

Mammalian

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCas49

Plasmid

#82391

Purpose

rbcL-sfGFP expressed from PccmK2 promoter with Gmr in glpK

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

rbcL-GFP

Promoter

PccmK2

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCAM17

Plasmid

#86473

Purpose

Expresses p12 and p9

Depositor

Pamela Silver

Article

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p12, p9

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCas4_1_2_VA

Plasmid

#186438

Purpose

Encodes Cas4, Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Promoter

T7

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCAT024

Plasmid

#171636

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-BFP
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSpCas9_human_ADAR2_ccC

Plasmid

#158112

Purpose

deletion hADAR2

Depositor

Emanuela Felley-Bosco

Article

Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

Insert

ADAR2 (ADARB1 Human)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS_2paLINX

Plasmid

#129655

Purpose

2P-activatable light-inducible nuclear exporter

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paLINX

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG_TWIST1

Plasmid

#215027

Purpose

Mammalian expression of human TWIST1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

twist family bHLH transcription factor 1 (TWIST1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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