We narrowed to 22,783 results for: Sis

1. pCold I-Hero45

   Plasmid

   #187931

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero45

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero45 (SERBP1 Human)
   

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD miniSOG

   Plasmid

   #91774

   Purpose

   Bacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

   Insert

   mini Singlet Oxygen Generator
   

   Tags

   Hexa-Histidine tag

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pV1393

   Plasmid

   #111430

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBV65

   Plasmid

   #108363

   Purpose

   A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

   Depositor

   Scott Moye-Rowley

   Article

   Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

   Insert

   loxP-natMX6-CgMET3prom-cre-loxP
   

   Use

   Gene disruption marker for candida glabrata

   Promoter

   C. glabrata MET3

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA NIA TLS1

   Plasmid

   #196980

   Purpose

   Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

   Depositor

   Friedrich Kragler

   Article

   Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   AtNIA1 gRNAs fused to TLS1 tags
   

   Expression

   Plant

   Promoter

   pU6-26, pU6-29

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-SaCas9-U6-sgSlc17a7

   Plasmid

   #124846

   Purpose

   Mutagenesis of Slc17a7

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc17a7 (Slc17a7 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMax_mOrange_G299C

   Plasmid

   #177829

   Purpose

   Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange_G299C
   

   Expression

   Mammalian

   Mutation

   G299C

   Promoter

   CMV

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHEE901-A3A

   Plasmid

   #229044

   Purpose

   nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.

   Depositor

   Huawei Zhang

   Article

   Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRelA*

   Plasmid

   #175590

   Purpose

   Tet inducible, KAN Resistant, fluor labelled (YFP) synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Catalytic domain of E.coli relA gene.
   

   Tags

   Fused with a flexible GS linker to mVenus

   Expression

   Bacterial

   Mutation

   Only the catalytic domain of the enzyme is used (…

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. HBT-CPK32-Flag

    Plasmid

    #107432

    Purpose

    For Arabidopsis mesophyll protoplast transient expression assay

    Depositor

    Jen Sheen

    Article

    Liu et al Nature. 2017 May 18;545(7654):311-316. d

    Insert

    CPK32 (CPK32 Mustard Weed)
    

    Tags

    Flag

    Expression

    Bacterial

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIRES-Puro-MGMT

    Plasmid

    #187654

    Purpose

    Expresses hMGMT together with the puromycin resistance gene via an IRES.

    Depositor

    Robert Sobol

    Article

    Li et al Cancers (Basel). 2022 Jul 22;14(15). pii

    Insert

    MGMT (MGMT Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHDE-35S-Cas9-mCherry-UBQ

    Plasmid

    #78932

    Purpose

    For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAs

    Depositor

    Yunde Zhao

    Article

    Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. NPY-td-Orange2

    Plasmid

    #83497

    Purpose

    Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

    Depositor

    Sebastian Barg

    Article

    Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

    Insert

    NPY (NPY Human)
    

    Tags

    tandem mOrange2

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC840 - pAAV 5xATF6 secNLuc

    Plasmid

    #82497

    Purpose

    Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

    Depositor

    Brandon Harvey

    Article

    Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

    Insert

    secNLuc
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    5x(ATF6 binding site) c-Fos minimal promoter

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Htr5b(3.7)-tTA-sv40

    Plasmid

    #184412

    Purpose

    AAV expression of tTA from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Htr5b gene promoter-tTA
    

    Use

    AAV

    Tags

    tTA

    Expression

    Mammalian

    Promoter

    Mouse Htr5b gene

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

    Plasmid

    #71719

    Purpose

    Expressing a GFP-tagged degron in the soma of C. elegans

    Depositor

    Abby Dernburg

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    auxin-responsive protein IAA17 (AXR3 Mustard Weed)
    

    Tags

    EmGFP

    Expression

    Worm

    Mutation

    minimal degron sequence (71-114aa) with start cod…

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7C-DNAJA1

    Plasmid

    #177655

    Purpose

    Recombinant protein synthesis of human DNAJA1

    Depositor

    Jeff Kuret

    Article

    Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

    Insert

    DNAJA1 (DNAJA1 Human)
    

    Tags

    6xHis-Thrombin

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. T/Brachyury-eGFP Rex Neo

    Plasmid

    #21222

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Brachyury promoter
    

    Use

    Lentiviral

    Tags

    eGFP

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. PBE-NG

    Plasmid

    #229045

    Purpose

    Modified nCas9 mediated-base editing with NG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R in Cas9.

    Depositor

    Huawei Zhang

    Article

    Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. CRY2(535, L348F)-mCherry

    Plasmid

    #75372

    Purpose

    Expresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherry

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CRY2 residues 1-535 (CRY2 Mustard Weed)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    L348F

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pTRE-Tight-FLAG-CYLD-WT

    Plasmid

    #60030

    Purpose

    WT CYLD under control of Tet promoter with N-terminal FLAG tag

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Nov. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA5/FRT/TO GFP DNAJB1

    Plasmid

    #19495

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB1 (DNAJB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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23. CRY2PHR(W349R)-mCherry

    Plasmid

    #75370

    Purpose

    Expresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherry

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CRY2 residues 1-498 (CRY2 Mustard Weed)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    W349R

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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24. pGMCK-T38S38-750-luxAB-DAS+4

    Plasmid

    #49518

    Purpose

    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    T38S8-P750-luxAB-DAS+4
    

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Promoter

    P750

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMBEC2

    Plasmid

    #187596

    Purpose

    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistance

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    nCas9-BEC-UGI; cas6f, gfp
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. NPY-pHTomato

    Plasmid

    #83501

    Purpose

    Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

    Depositor

    Sebastian Barg

    Article

    Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

    Insert

    NPY (NPY Human)
    

    Tags

    pHTomato

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMax_mOrange_A215C

    Plasmid

    #177828

    Purpose

    Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange_A215C
    

    Expression

    Mammalian

    Mutation

    A215C

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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28. Gal4BD-CRY2(535)

    Plasmid

    #75369

    Purpose

    Yeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)

    Depositor

    Chandra Tucker

    Article

    Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Tags

    Gal4BD

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. GFP-CYLD-STOP

    Plasmid

    #60077

    Purpose

    N-terminal GFP tagged CYLD with stop codon, driven by CMV promoter

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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30. hHSF1 K80Q

    Plasmid

    #118351

    Purpose

    Plasmid expresses hHSF1 with K80 in DBD mutated to Q, which abolish DNA-binding activity of HSF1.

    Depositor

    Lea Sistonen

    Article

    Westerheide et al Science. 2009 Feb 20. 323(5917):1063-6.

    Insert

    hHSF1 (HSF1 Human)
    

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K80Q

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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31. pHLuc (Antares-SEPLuc C1A4E+6)

    Plasmid

    #141089

    Purpose

    final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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32. pBtPIPLC His

    Plasmid

    #173808

    Purpose

    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Mary F Roberts, Anne Gershenson

    Article

    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

    Insert

    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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33. pMax_GFP_C289T

    Plasmid

    #177826

    Purpose

    Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP_C289T
    

    Expression

    Mammalian

    Mutation

    C289T

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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34. pQE-BR1

    Plasmid

    #160333

    Purpose

    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.

    Depositor

    Sang Taek Jung

    Article

    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

    Insert

    BR1
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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35. pCDFDuet-AtUBC8-S-AtUBA1-S

    Plasmid

    #222714

    Purpose

    Expression AtUBA1-S and AtUBC8-S in in bacterial cells

    Depositor

    Lei Wu

    Article

    Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

    Inserts

    AtUBC8 (UBC8 Mustard Weed)
    ATUBA1 (UBA1 Mustard Weed)
    

    Tags

    S-Tag

    Expression

    Bacterial

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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36. pICE-NLS-mCherry

    Plasmid

    #60364

    Purpose

    Plasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Sébastien Britton, Patrick Calsou

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    NLS-mCherry
    

    Tags

    NLS from SV40 T antigen and mCherry

    Promoter

    CMV-tet

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. mRFP-mem

    Plasmid

    #55622

    Purpose

    This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    mRFP-Mem
    

    Tags

    The N-terminal 20 residues of neuromodulin are fu…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5/FRT/TO DNAJB1

    Plasmid

    #19468

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB1 (DNAJB1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMax_BFP_A191G

    Plasmid

    #177823

    Purpose

    Site-specific mutagenesis of tagBFP for the purposes of creating a site-specific U:A mispair for the purposes of measuring UDG activity via Host Cell Reactivation (HCR).

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    tagBFP_A191G
    

    Expression

    Mammalian

    Mutation

    A191G

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDIV301

    Plasmid

    #204969

    Purpose

    AMA1 plasmid with Aspergillus optimized Mad7 and ble (bleomycin) resistance marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Inserts

    Mad7
    ble (bleomycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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