We narrowed to 34,413 results for: CaS;

1. pX165-LZ3 Cas9

   Plasmid

   #140561

   Purpose

   Expresses LZ3 Cas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   N690C T769I G915M N980K

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CARPID dCasRx-BASU

   Plasmid

   #153303

   Purpose

   express CARPID dCasRx-BASU fusion protein in mammalian cells

   Depositor

   Jian Yan, Liang Zhang

   Article

   Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

   Insert

   dCasRx-BASU
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   R239A/H244A/R858A/H863A in RfxCas13d

   Promoter

   EF-1a

   Available since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDNR-SpCas9-Cdt1

   Plasmid

   #80426

   Purpose

   Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Cdt1
   

   Use

   CRISPR

   Tags

   Cdt1

   Expression

   Mutation

   codon optimized for human

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. M3D395A-M14-dCas9

   Plasmid

   #134780

   Purpose

   Expression of fusion protein M3D395A-M14-dCas9 in Mammalian cells

   Depositor

   Shu-Bing Qian

   Article

   Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

   Insert

   METTL3/METTL14 methyltransferase domain and inactive dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D395A

   Promoter

   CMV

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCas9-P2A-BFP

   Plasmid

   #149447

   Purpose

   Expresses Cas9 and BFP in a lentiviral vector.

   Depositor

   Jonathan Chernoff

   Article

   Budagyan et al Methods Mol Biol. 2021;2262:323-334. doi

   Insert

   P2A-BFP
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   In frame with Cas9

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Puro-C9orf72

   Plasmid

   #83439

   Purpose

   Expresses Cas9 and a gRNA targeting C9orf72

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   gRNA targeting human C9orf72 (C9orf72 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

   Plasmid

   #86613

   Purpose

   Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   Expression

   Mammalian

   Mutation

   K848A, K1003A, & R1060A

   Promoter

   CBh

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Cas9-NRCH

   Plasmid

   #136926

   Purpose

   Expresses Cas9-NRCH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9-NRCH
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIVT-dCas9-EGFP

   Plasmid

   #174442

   Purpose

   in vitro transcription template for dCas9-EGFP

   Depositor

   Michael Lampson

   Article

   Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

   Insert

   Cas9 Endonuclease Dead
   

   Use

   In vitro transcription template

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   T7 SP6

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. XLone-Cas9-2A-GFP

    Plasmid

    #170159

    Purpose

    Express Cas9 and GFP upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    Cas9
    

    Use

    Synthetic Biology; Piggybac

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G promoter

    Available since

    July 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDmAct5C::Cas9-2A-Neo

    Plasmid

    #176679

    Purpose

    Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    D. melanogaster Act5C

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6xHis-MBP-huBsCas12a

    Plasmid

    #174671

    Purpose

    Expresses 6xHis-MBP tagged BsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. RCASBP(A)-3xFlag-mHoxd13wt

    Plasmid

    #145288

    Purpose

    Avian Expression of murine wildtype Hoxd13 with N-terminal 3xFlag tag

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    Hoxd13(wt) (Hoxd13 Mouse)
    

    Use

    Viral avian expression

    Tags

    3xFlag

    Expression

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAWPSG-Ptet-nCas9-BE

    Plasmid

    #195739

    Purpose

    Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli

    Depositor

    Seung-Goo Lee

    Article

    Jeong et al Chem Eng J, 2023

    Insert

    Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet(Tetracycline inducible protein)

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-spCas9-pNeo

    Plasmid

    #214120

    Purpose

    Gene expression by Piggybac transposase in mammalian cells

    Depositor

    Sho Yoshimatsu

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon-optimized for mammalian cells

    Promoter

    Available since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. GluN1 (px330:2-guideCas9)

    Plasmid

    #169426

    Purpose

    Expression of Cas9 and 2 guides

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCasper4-g-osk-TRICK

    Plasmid

    #71244

    Purpose

    oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

    Depositor

    Anne Ephrussi

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    oskar (osk Fly)
    

    Use

    P element-mediated germline transformation

    Tags

    12xPP7 and 6xMS2

    Expression

    Insect

    Mutation

    Promoter

    osk

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9-CBE6a-V106W

    Plasmid

    #215824

    Purpose

    Express CBE6a-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6xHis-MBP-huErCas12a

    Plasmid

    #174685

    Purpose

    Expresses 6xHis-MBP tagged ErCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huErCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 6xHis

    Expression

    Bacterial

    Mutation

    Q926R mutation is observed as indicated from the …

    Promoter

    T7

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET23b-Casp6-His

    Plasmid

    #11823

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 6 (CASP6 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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