We narrowed to 2,124 results for: PAM

1. FRB_nSuMMVp

   Plasmid

   #118970

   Purpose

   Expresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domain

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split nSuMMVp in fusion with FRB
   

   Tags

   Myc tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCPP5052

   Plasmid

   #128729

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopAM1
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

   Plasmid

   #137827

   Purpose

   MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
   

   Tags

   3x FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. C3 luc (T1 del)

   Plasmid

   #11358

   Depositor

   Donald McDonnell

   Article

   Fan et al Mol Endocrinol. 1996 Dec . 10(12):1605-1

   Insert

   C3 promoter (C3 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-AU1-mTOR-E2419K

   Plasmid

   #19994

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   mammalian target of rapamycin-E2419K (Mtor Rat)
   

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   Changed Glutamine 2419 to Lysine

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRB_LD 0_CS G_tTA-BFP

   Plasmid

   #58876

   Purpose

   MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
   

   Use

   AAV

   Tags

   EBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pATmin-GG

   Plasmid

   #191352

   Purpose

   Clostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Insert

   Plac-RFP (IPTG-inducible red fluorescent protein)
   

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. N1-mSin1.2-GFP

   Plasmid

   #72908

   Purpose

   GFP-tagged mSin1 (isoform 2)

   Depositor

   Ivan Yudushkin

   Article

   Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

   Insert

   Target of rapamycin complex 2 subunit MAPKAP1, isoform 2 (MAPKAP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-AU1-mTOR-L1460P

   Plasmid

   #19995

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   mammalian target of rapamycin-L1460P (Mtor Rat)
   

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   Changed Leucine 1460 to Proline

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pJK511

    Plasmid

    #155384

    Purpose

    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 S6K2 E388 D3E

    Plasmid

    #17731

    Depositor

    John Blenis

    Article

    Lee-Fruman et al Oncogene. 1999 Sep 9. 18(36):5108-14.

    Insert

    S6K2 (RPS6KB2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T388E, D3E(S410D, S417D, S423D): rapamycin-resist…

    Promoter

    CMV

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. FRB_nSbMVp

    Plasmid

    #118969

    Purpose

    Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nSbMVp in fusion with FRB
    

    Tags

    Myc tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. N1-mSin1.5-GFP

    Plasmid

    #72909

    Purpose

    GFP-tagged mSin1 (isoform 5)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Target of rapamycin complex 2 subunit MAPKAP1, isoform 5 (MAPKAP1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. RapR-Shp2-mVenus-flag

    Plasmid

    #188655

    Purpose

    Rapamycin regulated Shp2 phosphatase with mVenus and flag tag

    Depositor

    Andrei Karginov

    Article

    Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

    Insert

    Shp2
    

    Tags

    Flag and mVenus

    Expression

    Mammalian

    Mutation

    iFKBP insertion at V406 with deletion of residues…

    Promoter

    CMV

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. FRB_nPPVp

    Plasmid

    #118966

    Purpose

    Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split nPPVp in fusion with FRB
    

    Tags

    Myc tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK503

    Plasmid

    #155380

    Purpose

    dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    May 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. gCOTS-pyl

    Plasmid

    #92048

    Purpose

    This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.

    Depositor

    Lital Alfonta

    Article

    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

    Inserts

    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    

    Use

    Cyanobacteria s. elongatus genome recombination i…

    Promoter

    LeuP (native S. elongatus promoter) and PrcbL

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. MTK3_020

    Plasmid

    #123735

    Purpose

    Encodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    ERT2-fkbp-spdCa9(205-1368)
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CyPet-FKBP-E2

    Plasmid

    #175244

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK526

    Plasmid

    #155387

    Purpose

    PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA4 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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