176,453 results

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pAAV-mTH-GFP

Plasmid

#99128

Purpose

An AAV genome that expresses GFP from the mouse TH promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

mTH

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAnc80L65AAP

Plasmid

#92307

Purpose

Adeno-associated Viral Vector (AAV) capsid Anc80L65 in AAV2Rep expression construct with endogenous AAP

Depositor

Luk Vandenberghe

Publication

Landegger et al Nat Biotechnol. 2017 Mar;35(3):280-284.

Insert

Ancestral AAV Capsid Anc80L65AAP

Use

AAV and Synthetic Biology

Expression

Mammalian

Promoter

Rep2

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pTRIP-CMV-tagRFP-FLAG-cGAS

Plasmid

#86676

Purpose

Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Publication

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

tagRFP-FLAG-cGAS

Use

Lentiviral

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA-RLuc8

Plasmid

#87121

Purpose

Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.

Depositor

Sanjiv Sam Gambhir

Publication

Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Mutation

A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Publication

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CAG and TRE

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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Fuw-dCas9-Dnmt3a_IM

Plasmid

#84478

Purpose

Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

Depositor

Rudolf Jaenisch

Publication

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a_IM

Use

Lentiviral

Expression

Mammalian

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET30-2-GAPDH

Plasmid

#83910

Purpose

stable overexpression

Depositor

David Sabatini

Publication

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pMAL-his-LbCpf1-EC

Plasmid

#79008

Purpose

Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Publication

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

LbCpf1

Tags

MBP-6xHis-NLS

Expression

Bacterial

Promoter

tac promoter

Available since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pQCXIP-BSR-GFP11

Plasmid

#68716

Purpose

Split GFP assay

Depositor

Yutaka Hata

Publication

Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81.

Insert

bsr

Use

Retroviral

Tags

FLAG and GFP11

Expression

Mammalian

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

22 Articles

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pDD287 (mKate2^SEC^3xMyc

Plasmid

#70685

Purpose

mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Publication

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mKate2

Expression

Worm

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pBeloBac11loxP2272

Plasmid

#60342

Purpose

This is a BAC vector that contains both wt and mutant loxP sites.

Depositor

Jon Coren

Publication

Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

Type

Empty backbone

Use

Cre/Lox

Expression

Bacterial

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1_pMagFast2(3x)-iRFP

Plasmid

#67297

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Publication

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

pMagFast2(3x)-iRFP

Expression

Mammalian

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ptf-Galectin3

Plasmid

#64149

Purpose

Expression in mammalian cells

Depositor

Tamotsu Yoshimori

Publication

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin3 (LGALS3 Human)

Tags

EGFP and mRFP

Expression

Mammalian

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCRII_TOPO_hNEAT1

Plasmid

#61518

Purpose

human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

Depositor

Archa Fox

Publication

Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

Insert

NEAT1 (NEAT1 Human)

Use

General cloning vector

Mutation

*see comment below

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV-Ef1a-fDIO EYFP

Plasmid

#55641

Purpose

Flp-Dependent EYFP

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has service

AAV1, AAV2, AAV5, AAV8, AAV9, and AAV Retrograde

Insert

EYFP

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

31 Articles

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pAAV-CaMKIIa-EGFP

Plasmid

#50469

Purpose

EGFP under the control of CaMKIIa promoter

Depositor

Bryan Roth

Has service

AAV1, AAV2, AAV5, AAV8, AAV9, AAV PHP.eB, and AAV Retrograde

Insert

EGFP

Use

AAV

Tags

N/A

Promoter

CaMKIIa

Available since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

74 Articles

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FLAG-Stx17

Plasmid

#45911

Depositor

Noboru Mizushima

Publication

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

Syntaxin 17 (STX17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pcDNA3.1-SC35-cMyc

Plasmid

#44721

Depositor

Kathleen Scotto

Publication

Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

Insert

SC35 (SRSF2 Human)

Tags

his and myc

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pEGFP-N1-RACK1

Plasmid

#41088

Depositor

Anna Huttenlocher

Publication

Cox et al Mol Biol Cell. 2003 Feb;14(2):658-69.

Insert

Receptor for activated C kinase (RACK1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pcDNA-D3cpv

Plasmid

#36323

Depositor

Amy Palmer, Roger Tsien

Publication

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D3cpv second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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LeGO-Cer2

Plasmid

#27338

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, Cerulean

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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LRP6-pCS2

Plasmid

#27242

Depositor

Xi He

Publication

Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Expression

Mammalian

Available since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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AAV-CAG-ChR2-GFP

Plasmid

#26929

Purpose

AAV-mediated expression of ChR2-GFP for neuronal excitation

Depositor

Edward Boyden

Publication

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2

Use

AAV; Adeno-associated virus

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCI-neo-sOVA

Plasmid

#25098

Depositor

Maria Castro

Publication

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

soluble ovalbumin

Expression

Bacterial and Mammalian

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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EdTP

Plasmid

#24311

Depositor

Roel Nusse

Publication

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)

Use

Lentiviral

Tags

FLAG

Mutation

31 first amino acids of hTcf4 have been deleted.

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3.1/hChR2(H134R)-EYFP

Plasmid

#20940

Purpose

Mammalian expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

Depositor

Karl Deisseroth

Publication

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pMSP1

Plasmid

#20060

Depositor

Stephen Sligar

Publication

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

synthetic gene of deletion mutant (1-43) of human…

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pRK5-HA GST RagC wt

Plasmid

#19304

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding C (RRAGC Human)

Tags

GST and HA

Expression

Mammalian

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pMXs-Oct3/4-IP

Plasmid

#15918

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBS hShh (CT#401)

Plasmid

#13996

Depositor

Cliff Tabin

Publication

Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

Insert

Shh (SHH Human)

Expression

Bacterial

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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