163,606 results

1. pAAV-FRT-EF1a-TVA-2A-N2cG

   Plasmid

   #172361

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. AiP12857 - pAAV-AiE0674m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2857)

   Plasmid

   #224013

   Purpose

   AiE0674m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSV2 neuNT

   Plasmid

   #10918

   Depositor

   Bob Weinberg

   Article

   Bargmann et al Cell. 1986 Jun 6. 45(5):649-57.

   Insert

   neuNT (Erbb2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 19, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-U6/sgClta-sfGFP-Clta

   Plasmid

   #200261

   Purpose

   U6 promoter expressing sgClta. Clta homology arms, sfGFP knock-in.

   Depositor

   Justin Eyquem

   Article

   Nyberg et al Cell. 2023 Jan 19;186(2):446-460.e19. do

   Insert

   sfGFP flanked by homology arms targeting Clta, additional U6 promoter expressing sgClta
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SAP145

   Plasmid

   #11239

   Depositor

   Robin Reed

   Article

   Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

   Insert

   SAP145 (SF3B2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 19, 2006

   Availability

   Academic Institutions and Nonprofits only

6. OpenCRISPR-1 sgRNA-16nt stem

   Plasmid

   #221566

   Purpose

   Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

   Depositor

   Ali Madani

   Article

   Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

   Insert

   OpenCRISPR-1 sgRNA 16nt stem loop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 28, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGEX2T E7

   Plasmid

   #13634

   Depositor

   Karl Munger

   Article

   Wu et al J Virol. 1993 Apr . 67(4):2402-7.

   Insert

   HPV 16 E7 (E7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pHIV-Luc-ZsGreen

   Plasmid

   #39196

   Depositor

   Bryan Welm

   Inserts

   Firefly Luciferase Luc2P
   ZsGreen
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha and EF1-alpha

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. CYP51A1 (3I3K)

   Plasmid

   #25161

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CYP51A1_cw_09 (CYP51A1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acid residues 54-502

   Promoter

   Available Since

   Aug. 24, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

10. p3036 GST-HPV-16 E6

    Plasmid

    #10849

    Depositor

    Peter Howley

    Article

    Huibregtse et al EMBO J. 1991 Dec . 10(13):4129-35.

    Insert

    HPV16 E6 (E6 Human papillomavirus)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pTetOFF SNCA-A53T

    Plasmid

    #215372

    Purpose

    All-in-one lentiviral construct for the dox repressible expression of the A53T mutant of alpha-synuclein.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ala53Thr

    Promoter

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MBP-CRY1-PHR

    Plasmid

    #26035

    Depositor

    Johann Deisenhofer

    Article

    Brautigam et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

    Insert

    CRY1 (CRY1 Mustard Weed)
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pBabe puro-eIF4E

    Plasmid

    #33252

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    eIF4E (EIF4E Human)
    

    Use

    Retroviral

    Tags

    untagged

    Expression

    Mammalian

    Mutation

    unmodified

    Promoter

    SV40

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

14. human beta-catenin pcDNA3

    Plasmid

    #16828

    Purpose

    Mammalian expression of human beta-catenin with Flag tag

    Depositor

    Eric Fearon

    Article

    Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. KE-009

    Plasmid

    #133441

    Purpose

    Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.

    Depositor

    Gerhard Wagner

    Insert

    EIF4H (EIF4H Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRST7-ER beta (short)

    Plasmid

    #11357

    Depositor

    Donald McDonnell

    Article

    Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

    Insert

    ER beta short (ESR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2006

    Availability

    Academic Institutions and Nonprofits only

17. HC078 (3xHA-Atg13)

    Plasmid

    #59544

    Purpose

    Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

    Insert

    ATG13 (ATG13 Budding Yeast)
    

    Use

    Tags

    3xHA

    Expression

    Bacterial and Yeast

    Mutation

    AvrII site created after start codon

    Promoter

    ATG13

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pRK5M-TIMP3

    Plasmid

    #31715

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    TIMP3 (TIMP3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PP7 guide

    Plasmid

    #86168

    Purpose

    Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    PP7 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pErbB2-ECFP

    Plasmid

    #66945

    Purpose

    Mammalian expression of rat ErbB2 tagged with ECFP

    Depositor

    Mads Lerdrup, Bo van_Deurs

    Article

    Lerdrup et al J Cell Sci. 2006 Jan 1;119(Pt 1):85-95.

    Insert

    ErbB2 (Erbb2 Rat)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

21. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CMV::TVA/tRFP

    Plasmid

    #164486

    Purpose

    Dual CMV promoter plasmid to express TVA and TurboRFP

    Depositor

    Hollis Cline

    Article

    Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

    Inserts

    TVA (CD320 Chicken)
    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    sCMV and sCMV IE94

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ATeam1.03-nD/nA/pcDNA3

    Plasmid

    #51958

    Purpose

    mammalian expression of ATeam1.03-nD/nA, a FRET-based ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Kotera et al ACS Chem Biol. 2010 Feb 19;5(2):215-22.

    Insert

    ATeam1.03-nD/nA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ClCry4 Y319D

    Plasmid

    #160649

    Purpose

    Expression of protein for various experiments including finding interaction partners

    Depositor

    Joseph Takahashi

    Article

    Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

    Insert

    Clcry4 PHR Y319D
    

    Use

    Tags

    Hexa his

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-Cas9-GFP

    Plasmid

    #86145

    Purpose

    Lentiviral vector expressing Cas9-GFP

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    Cas9-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLminP_Luc2P_RE59

    Plasmid

    #90404

    Purpose

    STAT3 - Sis-inducible element (SIE) gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    STAT3

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. YIp204-PADH1-AFB2

    Plasmid

    #99531

    Purpose

    S. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, TRP1 marker (for use with the AID degron system)

    Depositor

    Helle Ulrich

    Insert

    AFB2 (AFB2 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA4-ZfCry4

    Plasmid

    #136242

    Purpose

    Protein expression of Zebrafish (Zf) cytochrome 4 with a C terminal FLAG tag

    Depositor

    Aziz Sancar

    Article

    Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

    Insert

    ZfCry4
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D196H- Please see depositor comments below

    Promoter

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSCV-N 18E5

    Plasmid

    #37882

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    HPV 18 E5 (E5 HPV)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available Since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

30. FLT1-KDR

    Plasmid

    #86034

    Purpose

    chimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2

    Depositor

    Christie Thomas

    Article

    Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

    Insert

    human VEGFR1-VEGFR2 hybrid (FLT1, KDR Human)
    

    Use

    Tags

    HA and t-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge